Recent advance of in vitro models in natural phytochemicals absorption and metabolism

Abstract

Natural phytochemicals absorption and metabolic process are mainly in the human gut. Simulating the absorption and metabolism of natural phytochemicals in vitro to predict the rate and degree of absorption of natural phytochemicals provides convenience for many researchers. However, in this process, many physiological factors <i>in vitro</i> are affected, such as stomach and intestinal juice composition, pH, intestinal transmission rate and so on. In recent years, the research methods have gradually improved to make these models more suitable for the natural phytochemicals absorption process, <i>in vitro</i> simulation models have become an essential means to study natural phytochemicals absorption. Therefore, this paper introduces the advantages and disadvantages of commonly used <i>in vitro</i> simulation models of natural phytochemicals absorption and metabolism, as well as briefly introduces the working principle of each model. To provide a theoretical basis for simulating natural phytochemicals absorption <i>in vitro</i> and development and utilization of natural phytochemicals.

Regulatory mechanisms of the green alga Ulva lactuca oligosaccharide via the metabolomics and gut microbiome in diabetic mice

“…Metabolites represent both the genome's downstream outputs and the environment's upstream inputs, enabling scientists to explore the nexus of gene and environmental interactions. As an emerging systems biology approach, metabolomics is now widely used in clinical and biomedical research to understand and construct blueprints of pathophysiological processes and search for diagnostic and prognostic biomarkers for various organisms ( Huang et al, 2021 ). Metabolic profiling and metabolic phenotyping can predict ongoing pathological processes, identify disease biomarkers as endogenous and exogenous metabolites, and provide unique insights into disease etiology ( Serag et al, 2021 ).…”

A 3D co-culture intestinal organoid system for exploring glucose metabolism

“…The effects of diet and nutrients on intestinal growth and development, ion and nutrient transport, secretory and absorption processes, the intestinal barrier, and the location-specific functions of the colon have all been studied using intestinal organoids as models ( Yin et al, 2019a ; Yin et al, 2019b ). Intestinal organoids can simulate the digestion and absorption of natural phytochemicals in the body, with the characteristics of substitution, simplification, and accuracy ( Huang et al, 2021 ).…”

Application of Caenorhabditis elegans in the evaluation of food nutrition: A review

“…At present, natural products were rich in active substances (such as polysaccharides, polyphenols and peptides), and the physiological functions of these active substances (such as antioxidation, antiaging, regulating lipid metabolism, improving immunity and anti‐inflammation) have attracted extensive attention (Huang et al, 2021; Q. Li, Li, Cai et al, 2022; M. Zhang et al, 2022; Zhao, Wu, et al, 2020). At the same time, it was necessary to use other biological models to study the nutritional activity of active substances from natural products before conducting human experiments, so a series of biological models such as flies, mice, rats, chickens, monkeys, and pigs have been developed (Wen et al, 2022).…”

“…At present, natural products were rich in active substances (such as polysaccharides, polyphenols and peptides), and the physiological functions of these active substances (such as antioxidation, antiaging, regulating lipid metabolism, improving immunity and antiinflammation) have attracted extensive attention (Huang et al, 2021;M. Zhang et al, 2022;Zhao, Wu, et al, 2020).…”