Recent advancement in nanocarriers for oral vaccination

Kour, Preeti; Rath, Goutam; Sharma, Gazal; Goyal, Amit K.

doi:10.1080/21691401.2018.1533842

Cited by 17 publications

(11 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The induction of mucosal immunity by subunit vaccines is a promising prophylactic strategy, which is especially effective against enteric infections (Ghaffari Marandi, Zolfaghari, Kazemi, Motamedi, & Amani, 2019; Qadri, Svennerholm, Faruque, & Sack, 2005; Shojaei Jeshvaghani et al, 2019). However, protein‐based oral vaccines require the protection of the antigen to ensure sufficient stability and transport to the intestine and the underlying immune system (Davitt & Lavelle, 2015; Kour, Rath, Sharma, & Goyal, 2018). Encapsulation of antigenic payload in nanoparticles made of chitosan or synthetic polymers like poly( dl ‐lactic‐co‐glycolic acid) (PLGA) provides such protection and has been used as a successful strategy to induce sIgA antibodies upon oral administration (Edelman et al, 1993; Fattal, Pecquet, Couvreur, & Andremont, 2002).…”

Section: Discussionmentioning

confidence: 99%

Plant‐derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells

Schwestka

Tschofen

Vogt

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

The encapsulation of biopharmaceuticals into micro‐ or nanoparticles is a strategy frequently used to prevent degradation or to achieve the slow release of therapeutics and vaccines. Protein bodies (PBs), which occur naturally as storage organelles in seeds, can be used as such carrier vehicles. The fusion of the N‐terminal sequence of the maize storage protein, γ‐zein, to other proteins is sufficient to induce the formation of PBs, which can be used to bioencapsulate recombinant proteins directly in the plant production host. In addition, the immunostimulatory effects of zein have been reported, which are advantageous for vaccine delivery. However, little is known about the interaction between zein PBs and mammalian cells. To better understand this interaction, fluorescent PBs, resulting from the fusion of the N‐terminal portion of zein to a green fluorescent protein, was produced in Nicotiana benthamiana leaves, recovered by a filtration‐based downstream procedure, and used to investigate their internalization efficiency into mammalian cells. We show that fluorescent PBs were efficiently internalized into intestinal epithelial cells and antigen‐presenting cells (APCs) at a higher rate than polystyrene beads of comparable size. Furthermore, we observed that PBs stimulated cytokine secretion by epithelial cells, a characteristic that may confer vaccine adjuvant activities through the recruitment of APCs. Taken together, these results support the use of zein fusion proteins in developing novel approaches for drug delivery based on controlled protein packaging into plant PBs.

show abstract

Section: Discussionmentioning

confidence: 99%

Plant‐derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells

Schwestka

Tschofen

Vogt

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Living delivery systems Recombinant bacteria [34][35][36][37][38][39][40][41][42][43][44] Viral vectors [45][46][47][48][49][50][51][52][53][54][55][56] Non-living delivery systems Virus-like particles [57] Micro-and nanoparticles [58][59][60] Lipid-based delivery systems [61][62][63][64][65][66][67][68][69] Nanogels [70] Targeted delivery M-cells Dectin-1 [71], GP2 [72], C5aR [73] Enterocytes FcRn [74] Aminopeptidase N [60,[75][76][77][78][79]…”

Section: Delivery Systemsmentioning

confidence: 99%

“…Furthermore, the adjuvant functions of other immunomodulators can be enhanced. Both synthetic and natural materials exist with different physicochemical properties, providing versatile options for oral vaccine design [58,59]. Natural materials include chitosan, starch, alginate, cellulose, β-glucan yeast ghost particles or biosynthesized poly β-hydroxybutyrate.…”

Section: Micro-and Nanoparticlesmentioning

confidence: 99%

“…Synthetic materials include polyurethane, polylactic acid, poly (lactic-co-glycolic acid) (PLGA) and polymethyl methacrylate resins [60,[174][175][176][177][178]. Especially natural materials have been extensively investigated for oral vaccine delivery due to their high biodegradability and compatibility, low to non-existing toxicity, strong adjuvanticity effects and their ability to retain the conformational structure of their loaded antigen [59]. Cellulose acetate phthalate (Eudragit) for example is often used as a polymer film to protect a capsulated vaccine as it is insoluble at the low pH of the stomach, but dissolves readily at higher pH in the gut, depending on the type of Eudragit [179].…”

Section: Micro-and Nanoparticlesmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Oral Subunit Vaccine Design

2020

View full text Add to dashboard Cite

Many pathogens invade the host at the intestinal surface. To protect against these enteropathogens, the induction of intestinal secretory IgA (SIgA) responses is paramount. While systemic vaccination provides strong systemic immune responses, oral vaccination is the most efficient way to trigger protective SIgA responses. However, the development of oral vaccines, especially oral subunit vaccines, is challenging due to mechanisms inherent to the gut. Oral vaccines need to survive the harsh environment in the gastrointestinal tract, characterized by low pH and intestinal proteases and need to reach the gut-associated lymphoid tissues, which are protected by chemical and physical barriers that prevent efficient uptake. Furthermore, they need to surmount default tolerogenic responses present in the gut, resulting in suppression of immunity or tolerance. Several strategies have been developed to tackle these hurdles, such as delivery systems that protect vaccine antigens from degradation, strong mucosal adjuvants that induce robust immune responses and targeting approaches that aim to selectively deliver vaccine antigens towards specific immune cell populations. In this review, we discuss recent advances in oral vaccine design to enable the induction of robust gut immunity and highlight that the development of next generation oral subunit vaccines will require approaches that combines these solutions.

show abstract

“…IgA and IgG, respectively [8]. It is one of the most accepted route for delivery of antigens safely almost without undesired toxicity; while, maintaining the integrity of antigen is very much challenging [9].…”

Section: Introductionmentioning

confidence: 99%

M-cell Targeting Acid-resistant Oral Vaccine Delivery for Immunization Against Hepatitis B Infection Using Cationic Solid Lipid Nanoparticles

Saraf

Sahoo

Jain³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Viral infection caused by Hepatitis B is transmitted by permucosal or parenteral exposure and also one of the prime causes of hepatocellular carcinoma and liver cirrhosis. Objectives: M-cell targeting acid-resistant oral vaccine delivery have been formulated for immunization against Hepatitis B infection. Methods: Cationic solid lipid nanoparticles (cSLNs) were prepared utilizing solvent injection technique. Hepatitis B surface antigen (HBsAg) loaded alginate coated cSLNs were anchored with lipopolysaccharide (LPS). SDS-PAGE was performed to evaluate acid degradation protection of prepared formulation. Induction of immunity produced by prepared nanoparticle for Hepatitis B was determined on female Balb/c mice followed by ELISA assays for assessing anti-HBsAg IgG/IgA antibodies in mucosal fluids. Results: Sustained release of HBsAg (60.66 %) has been exhibited from alginate coated cSLNs in comparison to cSLNs without alginate coating (97.72 %) after 48 h. The production of anti-HBs titer in intestinal, salivary and vaginal secretions was 3.41 IU/ml, 3.1 IU/ml and 2.51 IU/ml respectively in comparison to the control group. Integrity of the M-cells has been maintained after binding with SLN, and oral administration delivered the antigen to the desired site of gut. Conclusion: It was found effective in producing antibodies in mucosal immunization against Hepatitis B virus. So, this formulation could be used as a promising alternative preexisting vaccine to prevent Hepatitis B infection.

show abstract

Recent advancement in nanocarriers for oral vaccination

Cited by 17 publications

References 72 publications

Plant‐derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells

Plant‐derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells

Advances in Oral Subunit Vaccine Design

M-cell Targeting Acid-resistant Oral Vaccine Delivery for Immunization Against Hepatitis B Infection Using Cationic Solid Lipid Nanoparticles

Contact Info

Product

Resources

About