Recent advancements in polymeric nanofibers for ophthalmic drug delivery and ophthalmic tissue engineering

Sakpal, Darshana; Gharat, Sankalp; Momin, Munira

doi:10.1016/j.bioadv.2022.213124

Cited by 17 publications

(11 citation statements)

References 119 publications

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“…Nanofibers (NFs) composed of various polymers that are electrospun such as, blended hyaluronic acid/PVA inserts or bioactive self‐assembled NFs, for example, composed of laminin and fibronectin have been successfully used to prolong drug delivery for ophthalmic bioengineering of therapeutics. 50 , 51 , 52 , 53 NFs, nanoparticles, and chemical implants 52 can be engineered to be transparent, have optimal local release characteristics, and incorporate bioactive therapeutic agents of diverse functions including antibiotics and steroids. 54 Recently, we electrospun CALR into PCL/Collagen 1 NFs with full retention of cutaneous wound healing‐related biological activities by human dermal keratinocytes and fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

Calreticulin accelerates corneal wound closure and mitigates fibrosis: Potential therapeutic applications

Mishra,

Manzanares,

Prater

et al. 2023

J Cellular Molecular Medi

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The processes involved in regeneration of cutaneous compared to corneal tissues involve different intrinsic mechanisms. Importantly, cutaneous wounds involve healing by angiogenesis but vascularization of the cornea obscures vision. Previous studies showed that topically applied calreticulin (CALR) healed full‐thickness excisional animal wounds by a tissue regenerative process markedly enhancing repair without evoking angiogenesis. In the current study, the application of CALR in a rabbit corneal injury model: (1) accelerated full wound closure by 3 days (2) accelerated delayed healing caused by corticosteroids, routinely used to prevent post‐injury inflammation, by 6 days and (3) healed wounds without vascularization or fibrosis/hazing. In vitro, CALR stimulated proliferation of human corneal epithelial cells (CE) and corneal stromal cells (keratocytes) by 1.5‐fold and 1.4‐fold, respectively and induced migration of CE cells and keratocytes, by 72% and 85% compared to controls of 44% and 59%, respectively. As a marker of decreased fibrosis, CALR treated corneal wounds showed decreased immunostaining for α‐smooth muscle actin (α‐SMA) by keratocytes and following CALR treatment in vitro, decreased the levels of TGF‐β2 in human CE cells and α‐SMA in keratocytes. CALR has the potential to be a novel therapeutic both, to accelerate corneal healing from various injuries and in conjunction with corticosteroids.

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Section: Discussionmentioning

confidence: 99%

Calreticulin accelerates corneal wound closure and mitigates fibrosis: Potential therapeutic applications

Mishra,

Manzanares,

Prater

et al. 2023

J Cellular Molecular Medi

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“…51 The method described in this study has been used to synthesize core/sheath structures of polysulfide/poly(ethylene oxide) (PEO) and poly(dodecylthiophene)/PEO nanofibers. 44,52 The electrospinning process occurred rapidly, resulting in insufficient time for the two fluids to thoroughly mix before the fibers solidified. 35…”

Section: Covolatilizationmentioning

confidence: 99%

“…47,63 Nevertheless, they are unable to enhance cell adhesion, proliferation, and differentiation, whereas natural polymers typically exhibit superior bioactivities despite having inferior mechanical strength. 52,68 Researchers have shown a tendency to combine synthetic and natural polymers using cospinning, coaxial electrospinning, and surface coating modification. 35,50 CS, GEL, and COL are incorporated to markedly decrease the hydrophobic nature of nanofibers and enhance cell adhesion.…”

Section: Materials Of Electrospun Nanofiber Scaffoldsmentioning

confidence: 99%

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

Wu,

Yu,

Shao

et al. 2024

ACS Appl. Bio Mater.

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Skin tissue engineering (STE) is widely regarded as an effective approach for skin regeneration. Several synthetic biomaterials utilized for STE have demonstrated favorable fibrillar characteristics, facilitating the regeneration of skin tissue at the site of injury, yet they have exhibited a lack of in situ degradation. Various types of skin regenerative materials, such as hydrogels, nanofiber scaffolds, and 3D-printing composite scaffolds, have recently emerged for use in STE. Electrospun nanofiber scaffolds possess distinct advantages, such as their wide availability, similarity to natural structures, and notable tissue regenerative capabilities, which have garnered the attention of researchers. Hence, electrospun nanofiber scaffolds may serve as innovative biological materials possessing the necessary characteristics and potential for use in tissue engineering. Recent research has demonstrated the potential of electrospun nanofiber scaffolds to facilitate regeneration of skin tissues. Nevertheless, there is a need to enhance the rapid degradation and limited mechanical properties of electrospun nanofiber scaffolds in order to strengthen their effectiveness in soft tissue engineering applications in clinical settings. This Review centers on advanced research into electrospun nanofiber scaffolds, encompassing preparation methods, materials, fundamental research, and preclinical applications in the field of science, technology, and engineering. The existing challenges and prospects of electrospun nanofiber scaffolds in STE are also addressed.

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“…for more extensive information on tissue engineering applications for retinal regeneration. 42,43 3 | SKIN/WOUND HEALING…”

Section: Retinamentioning

confidence: 99%

“…We direct the readers to recent reviews by Sakpal et. al and Rizzolo et al for more extensive information on tissue engineering applications for retinal regeneration 42,43 …”

Section: Ophthalmologymentioning

confidence: 99%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

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Recent advancements in polymeric nanofibers for ophthalmic drug delivery and ophthalmic tissue engineering

Cited by 17 publications

References 119 publications

Calreticulin accelerates corneal wound closure and mitigates fibrosis: Potential therapeutic applications

Calreticulin accelerates corneal wound closure and mitigates fibrosis: Potential therapeutic applications

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

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