Recent advancements on use of CRISPR /Cas9 in maize yield and quality improvement

Abstract

CRISPR/Cas is a genome editing technique, permits accurate improvement of fiscally significant yield species by transgenic and non-transgenic strategies. We have reviewed CRISPR/Cas9 with or without DNA solution design in both maize as samples to redesign tolerance against dry season obstruction, improving seed’s oil contents production, and a gift of herbicide strength. Fundamentally, by exploiting the technologies of CRISPR/Cas9, development with late advances in plant tissue culture can be brought directly into monetarily significant genotypes. The various crop species are major agricultural products and play an indispensable role in sustaining human life. Over a long period, breeders strove to increase crop yield and improve quality through traditional breeding strategies. Today, many breeders have achieved remarkable results using modern molecular technologies. Recently, a new gene-editing system named the clustered regularly interspaced short palindromic repeats CRISPR/Cas9 technology has also improved crop quality. It has become the most popular tool for crop improvement due to its versatility. It has accelerated crop breeding progress by its precision in specific gene editing. This review summarizes the current application of CRISPR/Cas9 technology in crop quality improvement. It includes the modulation in appearance, palatability, nutritional components, and other preferred traits of various crops. Assortment created through such CRISPR/Cas9 engaged advanced raising procedures can be muddled from the regularly happening assortment and appropriately should be quickly open for commercialization.

Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize

“…The 2020 Nobel Prize in Chemistry was awarded to the inventors of CRISPR/Cas9 gene editing technology, which is easier, faster, cheaper, more precise, and safer than other technologies. The number of maize varieties that have undergone genetic modification using the CRISPR/Cas system [173] has been increasing in recent years [174,175]. It is precisely because of this that simultaneous efforts are needed to make the best use of the excellent new varieties of GM maize and to improve the regulatory means after their entry into the market to ensure the application of the required standards.…”

Impact of Global Climate Change on Maize (Zea Mays): Physiological Responses and Modern Breeding Techniques

“…Plant molecular biology has facilitated rapid advancements in agriculture over the past few decades. Various strategies, for example, marker-helped choice, map-based quality cloning, genome altering, and grouped routinely interspaced short palindromic rehash (CRISPR)/CRISPR-related 9, Cas9, have been utilized for the determination and improvement of plant characteristics in different harvests (Gillani et al, 2021).…”

Breeding Drought-Tolerant Maize (Zea mays) Using Molecular Breeding Tools: Recent Advancements and Future Prospective

“…CRISPR/Cas9, a novel gene editing tool [134], is valuable in accelerating molecular breeding for abiotic stress tolerance in crops (Figure 3). The genome editing tools, transcription activator-like effector nucleases (TALENs), meganucleases, zinc-finger nucleases (ZFN), and CRISPR/Cas9 have ensured the targeted gene editing in plants [135][136][137].…”