Recent advances and challenges in food-borne allergen detection

Sena‐Torralba, Amadeo; Pallás-Tamarit, Yeray; Morais, Sergi; Maquieira, Ángel

doi:10.1016/j.trac.2020.116050

Cited by 69 publications

(46 citation statements)

References 125 publications

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“…Analytical methods for the detection of legumes in food in the scientific literature are restricted to lupine, peanut, and soy, belonging to the group of the 14 EU main allergens, and to a much lesser extent to pea. These methods are most commonly based on enzyme-linked immunosorbent assays (ELISA) [ 17 , 18 ], polymerase chain reaction (PCR) [ 17 , 18 ] or high-performance liquid chromatography—tandem mass spectrometry (HPLC-MS/MS) [ 19 , 20 ]. Commercial ELISA and nucleic acid-based kits are available for lupine, peanut, and soy [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…These methods are most commonly based on enzyme-linked immunosorbent assays (ELISA) [ 17 , 18 ], polymerase chain reaction (PCR) [ 17 , 18 ] or high-performance liquid chromatography—tandem mass spectrometry (HPLC-MS/MS) [ 19 , 20 ]. Commercial ELISA and nucleic acid-based kits are available for lupine, peanut, and soy [ 18 ]. In contrast to ELISA and PCR, mass spectrometric methods have the advantage of being high-throughput screening tools for the multiplex detection of target proteins in foods [ 19 , 21 ] and are less vulnerable to interferences in complex food matrices [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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A UHPLC-MS/MS Method for the Detection of Meat Substitution by Nine Legume Species in Emulsion-Type Sausages

Spörl

Speer²,

Jira

2021

Foods

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Meat substitution by legume proteins in various types of meat products is a common practice. A reliable detection and quantification of these additives is required to control food specifications, especially regarding food fraud. Consequently, a UHPLC-MS/MS method for the simultaneous detection of alfalfa (Medicago sativa), broad bean (Vicia faba), chickpea (Cicer arietinum), lentil (Lens culinaris), lupine (Lupinus albus and Lupinus angustifolius), pea (Pisum sativum), peanut (Arachis hypogaea), and soy (Glycine max) proteins in meat products was developed. After protein extraction and tryptic digestion, three marker peptides for each legume species were measured by multiple reaction monitoring (MRM) using an optimized extraction protocol. To the best of our knowledge, the marker peptides for alfalfa, broad bean, chickpea, and lentil have not been reported previously. Emulsion-type sausages with 0.1, 0.4, 0.7, 1.0, 1.3, 1.6, 1.9, 2.2, and 2.5% meat substitution by each legume species, representing the concentration range between inadvertently transferred cross-contaminations and the conscious use for meat substitution, were produced for matrix calibration. No false-positive results were recorded in blank samples. In the quantification of alfalfa, broad bean, chickpea, lentil, pea, peanut, and soy, 673 of 756 measuring data of the recovery rate in unknown sausages were in the accepted range of 80–120%.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A UHPLC-MS/MS Method for the Detection of Meat Substitution by Nine Legume Species in Emulsion-Type Sausages

Spörl

Speer²,

Jira

2021

Foods

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“…Electrochemical biosensors can be highlighted as representative examples of these devices [ 15 , 16 , 17 ]. The analysis of food allergens, adulterants and functional foods by cutting-edge advances at different molecular levels provides new insights in biosensing strategies [ 18 , 19 ]. Furthermore, innovative and user-friendly immunochemical tools have been developed to accomplish food control and identify fraudulent food manufacturing technologies [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels

2021

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Tracking unreported allergens in commercial foods can avoid acute allergic reactions. A 2-step electrochemical immunosensor was developed for the analysis of the peanut allergen Ara h 1 in a 1-h assay (<15 min hands-on time). Bare screen-printed carbon electrodes (SPCE) were used as transducers and monoclonal capture and detection antibodies were applied in a sandwich-type immunoassay. The short assay time was achieved by previously combining the target analyte and the detection antibody. Core/shell CdSe@ZnS Quantum Dots were used as electroactive label for the detection of the immunological interaction by differential pulse anodic stripping voltammetry. A linear range between 25 and 1000 ng·mL−1 (LOD = 3.5 ng·mL−1), an adequate precision of the method (Vx0 ≈ 6%), and a sensitivity of 23.0 nA·mL·ng−1·cm−2 were achieved. The immunosensor was able to detect Ara h 1 in a spiked allergen-free product down to 0.05% (m/m) of peanut. Commercial organic farming cookies and cereal and protein bars were tested to track and quantify Ara h 1. The results were validated by comparison with an ELISA kit.

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“…Finally, Maquieira [ 207 ] reviewed recent approaches, including the electrochemical biosensors, existing kits for foodborne allergen detection and cutting-edge applications by focusing on the sensitivity, selectivity, and applicability of current methods in food samples.…”

Section: Application Of Electrochemical Biosensors In Food Analysismentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Molecules

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Safety and quality are key issues for the food industry. Consequently, there is growing demand to preserve the food chain and products against substances toxic, harmful to human health, such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices, such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long analyses, expensive and complex procedures, and skilled personnel. Therefore, developing performant electrochemical biosensors can significantly support the screening of food chains and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in food screening and their challenges.

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Recent advances and challenges in food-borne allergen detection

Cited by 69 publications

References 125 publications

A UHPLC-MS/MS Method for the Detection of Meat Substitution by Nine Legume Species in Emulsion-Type Sausages

A UHPLC-MS/MS Method for the Detection of Meat Substitution by Nine Legume Species in Emulsion-Type Sausages

Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

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