Recent advances and challenges in the bioconversion of acetate to value-added chemicals

Sakuntala, Mutyala; Kim, Eun Jung

doi:10.1016/j.biortech.2022.128064

Cited by 17 publications

(11 citation statements)

References 126 publications

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“…putida also exhibited a longer lag phase (24 and 84 h at 200 and 300 mM acetate, respectively). The prolonged lag phase can be attributed to the acetate uncoupling theory, , in which the higher concentration of acetate anions disrupts the ionic balance within the cell.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, acetate, which is a nontraditional carbon feedstock for bioconversion, will be highlighted in industrial biotechnology because it is inexpensive and decoupled with the food supply chain. 15 Acetate has the potential to serve as a carbon source for the bioconversion of various compounds, including fatty acids, PHB, 3-hydroxy propionic acid, tyrosine, phloroglucinol, glutamate, mevalonic acid, glycolate, and succinate. 16 other hand, most studies reported that acetate accumulation is frequently toxic to microorganisms at high concentrations because it is a weak organic acid with a relatively small molecule size that can penetrate the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

“…Microbial electrosynthesis (MES) has recently been used to generate acetate from CO/CO 2 using renewable electricity. − Acetate is an important intermediate to implementing biological carbon capture and utilization (CCU) processes in practical applications. Therefore, acetate, which is a nontraditional carbon feedstock for bioconversion, will be highlighted in industrial biotechnology because it is inexpensive and decoupled with the food supply chain …”

Section: Introductionmentioning

confidence: 99%

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Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation

Sakuntala

Khandelwal

et al. 2023

ACS Omega

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Acetate is an end-product of anaerobic biodegradation and one of the major metabolites of microbial fermentation and lingo-cellulosic hydrolysate. Recently, acetate has been highlighted as a feedstock to produce value-added chemicals. This study examined acetate conversion to succinate by citrate synthase (gltA)-overexpressed Pseudomonas putida under microaerobic conditions. The acetate metabolism is initiated with the gltA enzyme, which converts acetyl-CoA to citrate. gltA-overexpressing P. putida (gltA-KT) showed an ∼50% improvement in succinate production compared to the wild type. Under the optimal pH of 7.5, the accumulation of succinate (4.73 ± 0.6 mM in 36 h) was ∼400% higher than that of the wild type. Overall, gltA overexpression alone resulted in 9.5% of the maximum theoretical yield in a minimal medium with acetate as the sole carbon source. This result shows that citrate synthase is important in acetate conversion to succinate by P. putida under microaerobic conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation

Sakuntala

Khandelwal

et al. 2023

ACS Omega

Self Cite

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“…Second, it exists in the liquid form, which has no concern about mass transfer. And last, it can be easily produced and obtained from abundant sources. ,,, These characteristics make acetate an attractive substrate for the biosynthetic production of value-added chemicals. − However, despite this potential, the application of acetate for metabolite production is relatively challenging because of its low assimilation rate and toxicity. − Furthermore, when acetate is used to produce any chemically derived tricarboxylic acid cycle (TCA) metabolite, the acetate-derived flux needs to branch into three points: the target pathway for chemical production, the TCA cycle for energy metabolism, and the glyoxylate shunt for anaplerosis. , These branched fluxes must be well orchestrated for optimal production performance, which requires delicate flux redistribution rather than relying on a simple on-and-off approach to inactivating or amplifying a particular gene.…”

Section: Introductionmentioning

confidence: 99%

Stepwise Flux Optimization for Enhanced GABA Production from Acetate in Escherichia coli

Beck,

Ye,

Hwang

et al. 2024

J. Agric. Food Chem.

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Strategic allocation of metabolic flux is essential for achieving a higher production performance in genetically engineered organisms. Flux optimization between cell growth and chemical production has led to the establishment of cost-effective chemical production methods in microbial cell factories. This effect is amplified when utilizing a low-cost carbon source. γ-Aminobutyric acid (GABA), crucial in pharmaceuticals and biodegradable polymers, can be efficiently produced from acetate, a costeffective substrate. However, a balanced distribution of acetate-derived flux is essential for optimizing the production without hindering growth. In this study, we demonstrated GABA production from acetate using Escherichia coli by focusing on optimizing the metabolic flux at isocitrate and α-ketoglutarate nodes. Through a series of flux optimizations, the final strain produced 2.54 g/L GABA from 5.91 g/L acetate in 24 h (0.43 g/g yield). These findings suggest that delicate flux balancing with the application of a cheap substrate can contribute to cost-effective production of GABA.

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“…However, many types of bacteria can also metabolize acetate; for example, Escherichia coli can convert acetate into a cetyl- C oA via acetate k in a se (AckA) and p hospho t ransacetyl a se (Pta), or through acetyl-CoA synthetase [ 9 ]. Both of these pathways require the consumption of ATP, but in Pseudomonas sp., Klebsiella pneumoniae , and Acetobacter , it has been observed that a cetate: s uccinyl- C oA t ransferase (ASCT) can transfer the CoA from succinyl-CoA to acetate to produce acetyl-CoA without consuming ATP [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Gene Expression of Ethanol and Acetate Metabolic Pathways in the Acinetobacter baumannii EmaSR Regulon

Huang,

Shu,

Lin

2024

Microorganisms

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Background: Previous studies have confirmed the involvement of EmaSR (ethanol metabolism a sensor/regulator) in the regulation of Acinetobacter baumannii ATCC 19606 ethanol and acetate metabolism. RNA-seq analysis further revealed that DJ41_568-571, DJ41_2796, DJ41_3218, and DJ41_3568 regulatory gene clusters potentially participate in ethanol and acetate metabolism under the control of EmaSR. Methods: This study fused the EmaSR regulon promoter segments with reporter genes and used fluorescence expression levels to determine whether EmaSR influences regulon expression in ethanol or acetate salt environments. The enzymatic function and kinetics of significantly regulated regulons were also studied. Results: The EmaSR regulons P2796 and P3218 exhibited > 2-fold increase in fluorescence expression in wild type compared to mutant strains in both ethanol and acetate environments, and PemaR demonstrated a comparable trend. Moreover, increases in DJ41_2796 concentration enhanced the conversion of acetate and succinyl-CoA into acetyl-CoA and succinate, suggesting that DJ41_2796 possesses acetate: succinyl-CoA transferase (ASCT) activity. The kcat/KM values for DJ41_2796 with potassium acetate, sodium acetate, and succinyl-CoA were 0.2131, 0.4547, and 20.4623 mM−1s−1, respectively. Conclusions: In A. baumannii, EmaSR controls genes involved in ethanol and acetate metabolism, and the EmaSR regulon DJ41_2796 was found to possess ASCT activity.

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Recent advances and challenges in the bioconversion of acetate to value-added chemicals

Cited by 17 publications

References 126 publications

Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation

Citrate Synthase Overexpression of Pseudomonas putida Increases Succinate Production from Acetate in Microaerobic Cultivation

Stepwise Flux Optimization for Enhanced GABA Production from Acetate in Escherichia coli

Gene Expression of Ethanol and Acetate Metabolic Pathways in the Acinetobacter baumannii EmaSR Regulon

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