Recent advances and remaining barriers to producing novel formulations of fungicides for safe and sustainable agriculture

Tleuova, Aiym; Wielogórska, Ewa; Talluri, V.S.S.L. Prasad; Štěpánek, František; Elliott, Christopher T.; Grigoriev, D. O.

doi:10.1016/j.jconrel.2020.07.035

Cited by 112 publications

(60 citation statements)

References 81 publications

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“…The sustainable use of pesticides is currently requested by the Directive 2009/128/EC, which addresses the reduction of the risks and the impacts of pesticide on human health and the environment through integrated pest management and the use of alternative approaches (e.g., reduction, substitution or non-chemical alternatives to pesticides). In Italy, on the basis of the Directive, different agronomical protocols to treat and protect vineyards from seasonal infections of fungi and molds, which are also frequently associated with climate changes, are used [1]. Even though agrochemicals should be extremely specific to their relative target organisms due to the mode of action, they are not always completely selective.…”

Section: Introductionmentioning

confidence: 99%

Toxicological Comparison of Mancozeb and Zoxamide Fungicides at Environmentally Relevant Concentrations by an In Vitro Approach

Lori

Tassinari

Narciso

et al. 2021

IJERPH

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Mancozeb (MZ) and zoxamide (ZOX) are fungicides commonly used in pest control programs to protect vineyards. Their toxic and genotoxic potential were investigated in vitro on HepG2 and A549 cell lines at environmentally relevant concentrations. Cytotoxicity, apoptosis, necrosis and intracellular reactive oxygen species (ROS), comet assay and a micronucleus test with CREST immunofluorescence were used. The expression of a panel of genes involved in apoptosis/necrosis (BAX/BCL2), oxidative stress (NRF2), drug metabolism (CYP1A1) and DNA repair (ERCC1/OGG1) was evaluated by real-time PCR. Both fungicides were cytotoxic at the highest tested concentrations (295.7 and 463.4 µM, respectively); MZ induced necrosis, ZOX did not increase apoptosis but modulated BAX and BCL2 expression, suggesting a different mechanism. Both compounds did not increase ROS, but the induction of CYP1A1 and NRF2 expression supported a pro-oxidant mechanism. The comet assay evidenced MZ genotoxicity, whereas no DNA damage due to ZOX treatment was observed. Positive micronuclei were increased in both cell lines treated with MZ and ZOX, supporting their aneugenic potential. ERCC1 and OGG1 were differently modulated, indicating the efficient activation of the nucleotide excision repair system by both fungicides and the inhibition of the base excision repair system by MZ. Overall, MZ confirmed its toxicity and new ZOX-relevant effects were highlighted.

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Section: Introductionmentioning

confidence: 99%

Toxicological Comparison of Mancozeb and Zoxamide Fungicides at Environmentally Relevant Concentrations by an In Vitro Approach

Lori

Tassinari

Narciso

et al. 2021

IJERPH

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“…Treatment using synthetic fungicide is not entirely effective because Foc can last a long time in the soil thanks to its chlamydosporic structure (Fig. 1.D) which is resistant to extreme environmental stress (Gupta et al, 2020).This is also considered uneconomical and a source of environmental pollution, so that alternative pathogen control with antagonistic microbes (biocontrol) is more promising and sustainable (Abbey et al, 2018;Fournier et al, 2020;Tleuova et al, 2020). In this study, a number of indigenous microbes showed antagonistic activity against Foc growth in vitro (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In practice, the use of synthetic fungicides by shallot farmers has not been fully effective because of the residue left on crops, environmental pollution, and killing other organisms that are not targeted. Moreover, the continuous use of synthetic fungicides can lead to the emergence of resistant pathogenic populations (Mehnaz et al, 2013;Fournier et al, 2020;Tleuova et al, 2020). Biological control using microbes that are antagonistic to pathogenic fungi is the right alternative because it does not have a negative impact on the environment (Lecomte et al, 2016;Jamil et al, 2020;Kalman et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Antagonistic Activity and Characterization of Indigenous Soil Isolates of Bacteria and Fungi Against Onion Wilt Incited by Fusarium sp

Asmawati

Jumadi

2021

Preprint

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Tuber rot disease due to phytopathogen Fusarium oxysporum f. sp. cepae (Foc) infection is one of the main factors causing the decreasing amount of global shallot production. This study aims to find bacteria and fungi candidates which have Foc antagonistic activity through in vitro tests using dual culture techniques. A total of five bacterial isolates and three fungal isolates isolated from the rhizosphere of healthy onion plants showed the ability to inhibit Foc growth. B1 and B4 bacterial isolates had an average inhibitory capability of 65.93% and 72.27% respectively. Whereas C1 and C2 fungal isolates have the ability to inhibit the growth of Foc by as much as 74.82% and 67.76% respectively. The four tested microbial isolates were able to significantly inhibit Foc activity in vitro based on the ANOVA test, with values α = 0.05, and n = 3. Molecular analysis based on 16S-rRNA markers showed bacterial isolates B1 and B4 have an evolutionary relationship with B. subtilis. Whereas fungi C1 and C2 have evolutionary relationships with Aspergillus tubingensis and Trichoderma asperellum respectively, based on internal transcribed spacer (ITS) gene markers. The results of this study can be used to develop indigenous microbial consortiums as biological control agents for phytopathogenic fungi Fusarium oxysporum f. sp. cepae (Foc) on shallots.

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“…This has prompted intensive research on developing biopesticides, including botanical fungicides [ 7 ], as a strategy for developing safer formulations to champion sustainable [ 6 ] and eco-friendly agriculture. Such developments are of great significance, especially in the face of a growing global population, climate change, and the increasing resistance of fungi to existing compounds [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Pyruvate Dehydrogenase E1 as a Potential Target against Magnaporthe oryzae through Experimental and Theoretical Investigation

Wang

et al. 2021

IJMS

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Magnaporthe oryzae (M. oryzae) is a typical cause of rice blast in agricultural production. Isobavachalcone (IBC), an active ingredient of Psoralea corylifolia L. extract, is an effective fungicide against rice blast. To determine the mechanism of IBC against M. oryzae, the effect of IBC on the metabolic pathway of M. oryzae was explored by transcriptome profiling. In M. oryzae, the expression of pyruvate dehydrogenase E1 (PDHE1), part of the tricarboxylic acid (TCA cycle), was significantly decreased in response to treatment with IBC, which was verified by qPCR and testing of enzyme activity. To further elucidate the interactions between IBC and PDHE1, the 3D structure model of the PDHE1 from M. oryzae was established based on homology modeling. The model was utilized to analyze the molecular interactions through molecular docking and molecular dynamics simulation, revealing that IBC has π-π stacking interactions with residue TYR139 and undergoes hydrogen bonding with residue ASP217 of PDHE1. Additionally, the nonpolar residues PHE111, MET174, ILE 187, VAL188, and MET250 form strong hydrophobic interactions with IBC. The above results reveal that PDHE1 is a potential target for antifungal agents, which will be of great significance for guiding the design of new fungicides. This research clarified the mechanism of IBC against M. oryzae at the molecular level, which will underpin further studies of the inhibitory mechanism of flavonoids and the discovery of new targets. It also provides theoretical guidance for the field application of IBC.

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Recent advances and remaining barriers to producing novel formulations of fungicides for safe and sustainable agriculture

Cited by 112 publications

References 81 publications

Toxicological Comparison of Mancozeb and Zoxamide Fungicides at Environmentally Relevant Concentrations by an In Vitro Approach

Toxicological Comparison of Mancozeb and Zoxamide Fungicides at Environmentally Relevant Concentrations by an In Vitro Approach

Antagonistic Activity and Characterization of Indigenous Soil Isolates of Bacteria and Fungi Against Onion Wilt Incited by Fusarium sp

Identification of Pyruvate Dehydrogenase E1 as a Potential Target against Magnaporthe oryzae through Experimental and Theoretical Investigation

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