Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering

Zhou, Jian; Li, Qi; Tian, Zhuang; Yao, Qi; Zhang, Mingzhu

doi:10.1016/j.mtbio.2023.100870

Cited by 9 publications

(3 citation statements)

References 195 publications

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“…17 As a result, the development of microtissue arrays using 3D biological printing technology has attracted significant attention. 18 According to the ASTM standard, 3D bio-printing technology can be divided into jetting-based, extrusion-based 19 and vat photopolymerization-based bioprinting. 20 For different applications, the appropriate printing method should be selected.…”

Section: Introductionmentioning

confidence: 99%

Digital light processing-based bioprinting of microtissue hydrogel arrays using dextran-induced aqueous emulsion ink

Xu,

Liao,

Liu

et al. 2024

Journal of Bioactive and Compatible Polymers

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As a microscale three-dimensional bionic model, a microtissue array is a promising method for disease modeling and drug screening. Digital light processing (DLP) 3D bio-printing technology with the capabilities of excellent printing speed and resolution shows great potential for fabricating microtissue arrays. However, the outcomes of the microtissue arrays using DLP are limited by the shortage of biomaterials. In this work, we prepare hydrogel microtissues by combining DLP printing technology with biphasic bio-ink consisting of gelatin methacrylate (GelMA) and dextran. GelMA/ dextran is mixed to form bio-inks with different volume ratios, which can achieve high-precision printing of various patterns. Due to the presence of dextran, the bio-inks form the internal porous structure of the hydrogel in the printed patterns. The pore size is related to the proportion of dextran in bio-inks, and the pore size of hydrogel becomes smaller with the increase of dextran ratio. In addition, the adipose-derived stem cells (ADSCs) cells encapsulated in the porous hydrogels can maintain a high survival rate of 98.01% after 3 days of culture, and the larger pore size in the hydrogels promotes cell migration. Combining the DLP printing system with the porous hydrogel allows multiple micro-size geometric pattern hydrogels to be printed simultaneously to form microtissue arrays. In this study, a new bio-ink suitable for microtissue arrays is proposed, and a simple, accurate, and high-throughput biological manufacturing method for microtissue arrays is developed, thus providing a valuable tool for drug screening and tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Digital light processing-based bioprinting of microtissue hydrogel arrays using dextran-induced aqueous emulsion ink

Xu,

Liao,

Liu

et al. 2024

Journal of Bioactive and Compatible Polymers

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“…[ 22–24 ] However, most reported 3D‐printed cartilage scaffolds require in vitro co‐cultivation with bone marrow mesenchymal stem cells (BMSCs) to facilitate articular cartilage defect repair. [ 25,26 ] Some researchers have developed a 3D scaffold construct consisting of a BMSC internal repository that mimics the bone marrow lumen by encapsulating stem cells, functionalized with peptide modifications to promote cell migration into externally differentiation‐induced 3D‐printed units, and facilitated regeneration of osteochondral units in a rabbit model. [ 27 ] But this approach adds the step of in vitro culture, making the repair process cumbersome.…”

Section: Introductionmentioning

confidence: 99%

“…In other studies, this cumbersome approach also prolonged the repair time. [ 25,26 ] Furthermore, in vitro cultivation of BMSCs can compromise their stemness and homing ability, often leading to uncontrolled contamination. [ 28–30 ] Consequently, this hampers the rapid personalized customization of cartilage tissue engineering and its implementation in clinical settings.…”

Section: Introductionmentioning

confidence: 99%

Rapid Customization of Biomimetic Cartilage Scaffold with Stem Cell Capturing and Homing Capabilities for In Situ Inductive Regeneration of Osteochondral Defects

Zeng,

Chen,

Wei

et al. 2024

Adv Funct Materials

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Abstract3D printing of articular cartilage tissue faces challenges like replicating its complex structure, time‐consuming in vitro stem cell culture, and a lack of robust in situ regeneration methods for osteochondral defects (OC). In response, an innovative approach utilizing pre‐designed bioink modular units for one‐step printing and immediate implantation is proposed, circumventing the need for prior in vitro stem cell cultivation. The resulting printed scaffold not only accurately reproduces the three‐layer structure and material gradient of articular cartilage tissue but also attains impressive compressive strength (6.3 MPa) through the reinforcement of hydroxyapatite nanofibers and the establishment of chemical bonds with hydrogels. Moreover, the scaffold integrates stem cell capturing and homing layers on its bottom and top layers via chemical crosslinking aptamer and loading poly (lactic‐co‐glycolic acid) (PLGA) nanospheres encapsulated with stromal cell‐derived factor‐1α (SDF‐1α), respectively. This design enables the specific capture of bone marrow mesenchymal stem cells (BMSCs) in vivo through aptamer interaction, followed by their mobilization to home in on the hyaline cartilage layer via the chemotaxis of SDF‐1α concentration gradient. Within the scaffold's microenvironment, these BMSCs undergo differentiation into distinct cells for each layer, effectively contributing to the repair of OC defects in rabbits.

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Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering.

Rodrigues,

Oliveira,

Sá

et al. 2024

Macromolecular Bioscience

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Significant progress has been made in tissue engineering (TE), aiming at providing personalized solutions and overcoming the current limitations of traditional tissue and organ transplantation. 3D bioprinting has emerged as a transformative technology in the field, able to mimic key properties of the natural architecture of the native tissues. However, most successes in the area are still limited to avascular or thin tissues due to the difficulties in controlling the vascularization of the engineered tissues. To address this issue, several molecules, biomaterials, and cells with pro‐ and anti‐angiogenic potential have been intensively investigated. Furthermore, different bioreactors capable to provide a dynamic environment for in vitro vascularization control have been also explored. The present review summarizes the main molecules and TE strategies used to promote and inhibit vascularization in TE, as well as the techniques used to deliver them. Additionally, it also discusses the current challenges in 3D bioprinting and in tissue maturation to control in vitro/in vivo vascularization. Currently, this field of investigation is of utmost importance and may open doors for the design and development of more precise and controlled vascularization strategies in TE.

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Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering

Cited by 9 publications

References 195 publications

Digital light processing-based bioprinting of microtissue hydrogel arrays using dextran-induced aqueous emulsion ink

Digital light processing-based bioprinting of microtissue hydrogel arrays using dextran-induced aqueous emulsion ink

Rapid Customization of Biomimetic Cartilage Scaffold with Stem Cell Capturing and Homing Capabilities for In Situ Inductive Regeneration of Osteochondral Defects

Molecules in Motion: Unravelling the Dynamics of Vascularization Control in Tissue Engineering.

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