Recent Advances in Antibiotic-Free Markers; Novel Technologies to Enhance Safe Human Food Production in the World

Mmbando, Gideon Sadikiel

doi:10.1007/s12033-022-00609-7

Cited by 3 publications

(3 citation statements)

References 114 publications

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“…Additionally, the majority of documented UVB-resistant transgenic plants or mutants have been developed primarily in controlled laboratory environments; further research is required to evaluate their performance in the field before commercialization. By using recently proposed antibiotic-free marker techniques, UVB-resistant crops can be produced without encountering public opposition or biosafety concerns [ 97 ].…”

Section: Discussionmentioning

confidence: 99%

“…UVB hyper-resistant varieties may be produced by breeding plants that can both avoid and repair DNA damage. Several recently proposed antibiotic-free marker-free techniques must be used in order to reduce public resistance to genetically modified UVB-resistant crops and biosafety worries [ 97 ]. To create super-hyper tolerant varieties, future research should investigate combining MAP kinase and ATR with DNA repair enzymes.…”

Section: Potential Strategies For Breeding Uvb-resistant Plantsmentioning

confidence: 99%

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The recent possible strategies for breeding ultraviolet-B-resistant crops

Mmbando

2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Section: Potential Strategies For Breeding Uvb-resistant Plantsmentioning

confidence: 99%

The recent possible strategies for breeding ultraviolet-B-resistant crops

Mmbando

2024

Heliyon

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“…Silver Oxynitrate [Ag(Ag 3 O 4 ) 2 NO 3 or Ag 7 NO 11 ] Silver oxynitrate is a silver oxysalt, containing stable higher oxidation state ion species of silver, and it is now being explored because of excellent effects observed in various types of research. It is not only effective against antibiotic-resistant and multi-drug-resistant mutants but has also been seen to target biofilms.…”

Section: Silver Ions and Silver Nanoparticlesmentioning

confidence: 99%

Combating Microbial Infections Using Metal-Based Nanoparticles as Potential Therapeutic Alternatives

Kaur

Kaur²,

Alyami

et al. 2023

Antibiotics

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The nature of microorganisms and the efficiency of antimicrobials have witnessed a huge co-dependent change in their dynamics over the last few decades. On the other side, metals and metallic compounds have gained popularity owing to their effectiveness against various microbial strains. A structured search of both research and review papers was conducted via different electronic databases, such as PubMed, Bentham, Springer, and Science Direct, among others, for the present review. Along with these, marketed products, patents, and Clinicaltrials.gov were also referred to for our review. Different microbes such as bacteria, fungi, etc., and their diverse species and strains have been reviewed and found to be sensitive to metal-carrying formulations. The products are observed to restrict growth, multiplication, and biofilm formation effectively and adequately. Silver has an apt use in this area of treatment and recovery, and other metals like copper, gold, iron, and gallium have also been observed to generate antimicrobial activity. The present review identified membrane disruption, oxidative stress, and interaction with proteins and enzymes to be the primary microbicidal processes. Elaborating the action, nanoparticles and nanosystems are shown to work in our favor in well excelled and rational ways.

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Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering

Súnico,

Piunti,

Bhattacharjee

et al. 2024

IJMS

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Selectable marker genes are useful for recognizing which cells have integrated specific sequences in their genome after genetic transformation processes. They are especially important for fruit trees genetic transformation to individuate putatively genetically modified events, because most of the protocols used to genetic engineer these species are often unsuccessful or with low efficiency. Traditional selectable marker genes, mainly of bacterial origin, confer antibiotics/herbicides-resistance or metabolic advantages to transformed cells. Genes that allow the visual recognition of engineered tissues without using any selective agent, such as morphogenic regulators and reporter genes, are also used as selection tools to in vitro identify genetically modified regenerated lines. As final step, genetic engineered plants should be tested in field conditions, where selectable marker genes are no longer necessary, and strongly unpopular especially for the commercial development of the new products. Thus, different approaches, mainly based on the use of site-specific recombinases and/or editing nucleases, are being now used to recover marker-free fruit crops. This review describes and comments the most used and suitable selection tools of interest, particularly for fruit tree genetic engineering. Lastly, a spotlight highlights the biosafety aspects related to the use of selectable marker genes exploited for fruit species genetic engineering.

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Recent Advances in Antibiotic-Free Markers; Novel Technologies to Enhance Safe Human Food Production in the World

Cited by 3 publications

References 114 publications

The recent possible strategies for breeding ultraviolet-B-resistant crops

The recent possible strategies for breeding ultraviolet-B-resistant crops

Combating Microbial Infections Using Metal-Based Nanoparticles as Potential Therapeutic Alternatives

Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering

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