Recent Advances in Bacterial Polyamine Transport Systems

Kurihara, Shin; Suzuki, Hideyuki

doi:10.1007/978-4-431-55212-3_14

Cited by 4 publications

(5 citation statements)

References 27 publications

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“…gracilis. The high degree of affinity of Hg for polyamines, the multitude of microbial uptake systems for polyamines − , and the fact that these systems have been reported to facilitate cation transport suggest that polyamine transport represents an underappreciated uptake pathway for Hg. Indeed, in addition to being part of biogenic algal DOM, polyamine compounds such as cadaverine and putrescine are typically produced during the anaerobic fermentative breakdown of amino acids from dead organisms possibly offering a novel route for Hg uptake in anoxic soils and sediments.…”

Section: Resultsmentioning

confidence: 99%

Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight

Mangal

Stenzler

Poulain

et al. 2018

Environ. Sci. Technol.

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The biological mobilization of mercury (Hg) into microbes capable of Hg methylation is one of the limiting steps in the formation of the neurotoxin methylmercury (MeHg). Although algal dissolved organic matter (DOM) has been associated with increased MeHg production, the relationship between bacterial Hg uptake and algal DOM remains unexplored. In this study, we aimed to address how the quantity and quality of DOM, freshly harvested from several algae, affected the bacterial uptake of Hg with the use of a biosensor capable of functioning both aerobically and anaerobically. We combined biosensor measurements with high-resolution mass spectrometry and field-flow fractionation to elucidate how DOM composition and molecular weight influenced microbial Hg uptake. We showed that freshly harvested DOM from Chlorophyte and Euglena mutabilis strongly inhibited aerobic and anaerobic Hg uptake, whereas DOM harvested from Euglena gracilis did not exhibit this same pronounced effect. Once fractionated, we found that amino acids and polyamines, most abundant in Euglena gracilis DOM, were positively correlated to increase Hg uptake, suggesting that these molecules are potentially underappreciated ligands affecting Hg bioavailability. As water quality is affected by eutrophication, algal community assemblages will change, leading to variations in the nature of autochthonous DOM released in aquatic systems. Our results highlight that variations in the emergent properties of DOM originating from varying algal species can have a profound effect on bacterial Hg uptake and thus methylation.

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Section: Resultsmentioning

confidence: 99%

Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight

Mangal

Stenzler

Poulain

et al. 2018

Environ. Sci. Technol.

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“…Similarly, methyl-accepting chemotaxis protein-encoding genes, the majority of the two-component system CheA family (chemotaxis-like) genes, the potABCD spermidine/putrescine transporter components, and KOs within the tight adherence export apparatus system were all more abundant in the plastic-associated metagenomes. These genes have been implicated in chemotaxis, signaling of swarming activity, surface motility, colonization, and biofilm formation ( 64 – 68 ) ( Fig. 9 ; see Data Set S1 in the supplemental material).…”

Section: Discussionmentioning

confidence: 99%

Diversity and Activity of Communities Inhabiting Plastic Debris in the North Pacific Gyre

et al. 2016

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Marine plastic debris is a growing concern that has captured the general public’s attention. While the negative impacts of plastic debris on oceanic macrobiota, including mammals and birds, are well documented, little is known about its influence on smaller marine residents, including microbes that have key roles in ocean biogeochemistry. Our work provides a new perspective on microbial communities inhabiting microplastics that includes its effect on microbial biogeochemical activities and a description of the cross-domain communities inhabiting plastic particles. This study is among the first molecular ecology, plastic debris biota surveys in the North Pacific Subtropical Gyre. It has identified fundamental differences in the functional potential and taxonomic composition of plastic-associated microbes versus planktonic microbes found in the surrounding open-ocean habitat.

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“…This confirms the presence of taxa harboring the spermidine‐preferential uptake system in the plankton of Lake Zurich, which corresponds with findings from marine metatranscriptomes (Mou et al ). Genes for the two major transport proteins were found in many phyla such as for example Proteobacteria, Cyanobacteria, or Actinobacteria (Mou et al ; Ghylin et al ; Kurihara and Suzuki ). This suggests that numerous microorganisms harbor multiple parallel transport systems to regulate the uptake of different polyamines according to their actual availability, as reflected by our kinetic measurements (Fig.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal distribution and microbial assimilation of polyamines in a mesotrophic lake

Krempaska

Horňák

2017

Limnology & Oceanography

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We examined seasonal and spatial variations of dissolved free polyamines (DFPA) in the large mesotrophic prealpine Lake Zurich (Switzerland). An ion‐pairing liquid chromatography method with mass spectrometric detection was optimized for the quantification of DFPA without prior concentration or derivatization. Total DFPA concentrations varied between 0.4 nM and 11 nM in winter and spring of 2015, respectively. Polyamine concentrations were highest in the epilimnion during the phytoplankton growth phase, and reflected occurrence patterns of diatoms. Putrescine consistently dominated the DFPA pool throughout the season whereas norspermidine was detected only once in spring. Incubations with 13C‐labeled putrescine revealed concentration‐dependent uptake rates (3.2–5 nM h−1) and short turnover times (2.4–4.4 h). In contrast, incorporation rates into biomass measured with 14C‐labeled putrescine were substantially lower (0.9 nM h−1), indicating that this compound was predominantly respired. Uptake kinetics moreover suggested that putrescine in situ uptake rates were limited by low ambient concentrations. Competition assays revealed that putrescine uptake could only be inhibited by excess concentrations of another polyamine (spermidine), but not by amino acids. Bacteria affiliated with Limnohabitans and the acI lineage of Actinobacteria were identified as the taxa with the highest fraction (25–32%) of putrescine‐incorporating cells. This compound was also utilized by Alphaproteobacteria, Cyclobacteriaceae, diatoms, and cyanobacteria. Overall, our data point to fast turnover of putrescine by different microorganisms, implying the importance of this substrate as an attractive energy source.

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Recent Advances in Bacterial Polyamine Transport Systems

Cited by 4 publications

References 27 publications

Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight

Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight

Diversity and Activity of Communities Inhabiting Plastic Debris in the North Pacific Gyre

Spatiotemporal distribution and microbial assimilation of polyamines in a mesotrophic lake

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