Recent Advances in Bacteriophage Based Biosensors for  Food-Borne Pathogen Detection

Singh, Amit; Poshtiban, Somayyeh; Evoy, Stéphane

doi:10.3390/s130201763

Cited by 310 publications

(205 citation statements)

References 82 publications

Supporting

Mentioning

201

Contrasting

Unclassified

Order By: Relevance

“…The phage S16 LTF-MBs were unaffected by excess background flora or variable buffer pH and salt concentrations (pH 4 to 9 and 0 to 1 M NaCl). This validates their suitability for in situ detection of Salmonella and seems superior to the more pH-sensitive and cross-reactivity-prone immunosorbent-based tests (17,61).…”

Section: Discussionmentioning

confidence: 85%

“…A negligible amount of TMB conversion was observed for the buffer-only control, with an A 450 value of 0.24 Ϯ 0.05, suggesting that a residual amount of HRP-LTF remained after washing; this was set as the background value of the assay. ELLTA was sensitive and the results statistically significant down to 10 2 CFU · ml Ϫ1 (A 450 of 0.77 Ϯ 0.18; t test with buffer-only control, P Ͻ 0.001), similar to other phage-and phage-encoded protein-based assays with detection limits ranging between 10 and 10 4 CFU · ml Ϫ1 (17). While the value for the lowest Salmonella concentration tested, 10 CFU · ml Ϫ1 (A 450 of 0.36 Ϯ 0.10), was higher than the value for the blank-only control (A 450 of 0.24), there was no statistical difference between the two values (P Ͼ 0.05).…”

mentioning

confidence: 76%

See 1 more Smart Citation

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Denyes

Dunne

Steiner

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

Bacteriophage-based assays and biosensors rival traditional antibodybased immunoassays for detection of low-level Salmonella contaminations. In this study, we harnessed the binding specificity of the long tail fiber (LTF) from bacteriophage S16 as an affinity molecule for the immobilization, enrichment, and detection of Salmonella. We demonstrate that paramagnetic beads (MBs) coated with recombinant gp37-gp38 LTF complexes (LTF-MBs) are highly effective tools for rapid affinity magnetic separation and enrichment of Salmonella. Within 45 min, the LTF-MBs consistently captured over 95% of Salmonella enterica serovar Typhimurium cells from suspensions containing from 10 to 10 5 CFU · ml Ϫ1 , and they yielded equivalent recovery rates (93% Ϯ 5%, n ϭ 10) for other Salmonella strains tested. LTF-MBs also captured Salmonella cells from various food sample preenrichments, allowing the detection of initial contaminations of 1 to 10 CFU per 25 g or ml. While plating of bead-captured cells allowed ultrasensitive but time-consuming detection, the integration of LTF-based enrichment into a sandwich assay with horseradish peroxidaseconjugated LTF (HRP-LTF) as a detection probe produced a rapid and easy-to-use Salmonella detection assay. The novel enzyme-linked LTF assay (ELLTA) uses HRP-LTF to label bead-captured Salmonella cells for subsequent identification by HRP-catalyzed conversion of chromogenic 3,3=,5,5=-tetramethylbenzidine substrate. The color development was proportional for Salmonella concentrations between 10 2 and 10 7 CFU · ml Ϫ1 as determined by spectrophotometric quantification. The ELLTA assay took 2 h to complete and detected as few as 10 2 CFU · ml Ϫ1 S. Typhimurium cells. It positively identified 21 different Salmonella strains, with no cross-reactivity for other bacteria. In conclusion, the phage-based ELLTA represents a rapid, sensitive, and specific diagnostic assay that appears to be superior to other currently available tests. IMPORTANCEThe incidence of foodborne diseases has increased over the years, resulting in major global public health issues. Conventional methods for pathogen detection can be laborious and expensive, and they require lengthy preenrichment steps. Rapid enrichment-based diagnostic assays, such as immunomagnetic separation, can reduce detection times while also remaining sensitive and specific. A critical component in these tests is implementing affinity molecules that retain the ability to specifically capture target pathogens over a wide range of in situ applications. The protein complex that forms the distal tip of the bacteriophage S16 long tail fiber is shown here to represent a highly sensitive affinity molecule for the specific enrichment and detection of Salmonella. Phage-encoded long tail fibers have huge potential for development as novel affinity molecules for robust and specific diagnostics of a vast spectrum of bacteria.

show abstract

Section: Discussionmentioning

confidence: 85%

mentioning

confidence: 76%

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Denyes

Dunne

Steiner

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…For biosensor purposes, bacteriophages have found utility as biorecognition agents for bacteria given the high degree of specificity and stability relative to antibodies (A. Singh, Poshtiban, & Evoy, 2013). Bacteriophages have also been used as biorecognition elements for a broader spectrum of targets through advances in phage display (Leeet al, 2013).…”

Section: Alternative Biorecognition Elements Bacteriophages and Recepmentioning

confidence: 99%

Developments in nanoparticles for use in biosensors to assess food safety and quality

Warriner

Reddy

Namvar

et al. 2014

Trends in Food Science & Technology

View full text Add to dashboard Cite

Highlights•Background to the evolution of biosensor based on nanoparticles.•Application of nanoparticles in biosensor devices.•Overview of biosensor formats applied in food testing.•Examples of integrated biosensor systems for food sampling.•Prospects and challenges in nanosensors in pathogen testing.The following will provide an overview on how advances in nanoparticle technology have contributed towards developing biosensors to screen for safety and quality markers associated with foods. The novel properties of nanoparticles will be described and how such characteristics have been exploited in sensor design will be provided. All the biosensor formats were initially developed for the health care sector to meet the demand for point-of-care diagnostics. As a consequence, research has been directed towards miniaturization thereby reducing the sample volume to nanolitres. However, the needs of the food sector are very different which may ultimately limit commercial application of nanoparticle based nanosensors. IntroductionThrough evolution we have developed senses to enable us to differentiate good foods from bad. The art of cheese making, brewing and viticulture has relied on the producer using their senses to assess quality. Establishing if a food was safe was more problematic and typically relied on tasters to ensure dishes provided to their masters were sound. Clearly, analytical testing is a more reliable and objective means of assessing the quality and safety of foods. Laboratory testing provided a basis for developing protocols and limits that could be applied to foods to define quality or safety. Yet, the expense and delay in acquiring results remain critical limitations. Consequently, there is an on-going demand for biosensors that can be used outside the laboratory environment to assess the safety and quality of foods on-site.In many instances the needs of the food industry mirrored that of the health sector where rapid diagnostics would enhance treatments and prognosis. The health sector provides a more lucrative market for diagnostic companies so it is not unexpected to find that the majority of research to date has been towards developing biosensors to meet the market demands. Consequently, those biosensors encountered in the food sector are derived from those devices initially fabricated to meet the needs of the health care sector. Within this context, the following review provides a brief history on how the field of biosensors developed and the contribution made by advances in nanoparticle technology. The efforts in transferring biosensor technologies to the food sector will be provided with a focus on safety, and to a degree, quality assessment. The review does not attempt to provide an exhaustive list of advances given the numerous quality reviews are already published in this area (Arugula and Simonian,

show abstract

“…Particular consideration should be given to the recognition type of the bioreceptor and the immobilization method. The selection of bioreceptors should be based on their ability to bind specific target materials and should consider their capacity for immobilization (Singh et al, 2013). Kisukuri and Andrade (2015).…”

Section: Biosensorsmentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

View full text Add to dashboard Cite

Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

show abstract

Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection

Cited by 310 publications

References 82 publications

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Developments in nanoparticles for use in biosensors to assess food safety and quality

Biosensors and their Applications in Food Safety: A Review

Contact Info

Product

Resources

About