Recent advances in biosensor based endotoxin detection

Das, Aradhana; Kumar, Pradeep; Swain, Shasank S.

doi:10.1016/j.bios.2013.07.020

Cited by 135 publications

(69 citation statements)

References 97 publications

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“…Endotoxins, chemically lipopolysaccharides usually found in food, environment, and clinical products of bacterial origin, are unavoidable ubiquitous microbiological contaminants [45]. An interesting review describes the application of different sensing principles including SPR and QCM for the detection of endotoxins.…”

Section: Endotoxinsmentioning

confidence: 99%

Label free Affinity sensing: application to food analysis

Scarano¹,

Mariani²,

Minunni³

2016

ACTA IMEKO

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In this paper real-time, label free sensing principle based on Surface Plasmon Resonance (SPR) and gravimetric, i.e. Quartz Crystal Microbalance (QCM) are described and some applications to food analysis problems are reported. Affinity receptors are introduced and examples using antibodies, nucleic acid probes and biomimetic receptors i.e. molecular imprinted polymers (MIP) reported. In particualr pesticide, endotoxins, pathogens, Genetically Modified Organism (GMOs) detection and food origin analysis are reviewed.

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Section: Endotoxinsmentioning

confidence: 99%

Label free Affinity sensing: application to food analysis

Scarano¹,

Mariani²,

Minunni³

2016

ACTA IMEKO

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“…If a loss of emitted energy occurs, the fluorescent emission wavelength is longer than the excitation wavelength and this is called the Stokes shift [49].…”

Section: Fluorescent Biosensorsmentioning

confidence: 99%

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

Kłos-Witkowska¹

2015

Pol. J. Environ. Stud.

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The last two decades has seen a significant increase in interest in biosensors. According to the Web of Science database, the number of scientific publications over the past 20 years increased from 309 in 1993 up to 3,467 in 2013 (Fig. 1).The demand for developing new detection methods, increasing interest in visualization techniques [1][2][3], emphasis placed on new drug discovery programs [4], diagnostic test development [5][6][7], and attempts to explain cellular action mechanisms and to trace a cell's metabolism pathway [7,8], and the possibility of using biosensors in numerous fields of application, (for example: medicine [9-11], environmental protection [12-14, 16-17], food industry [18], and defense industry [19]) has results in an increasing number of research projects targeted at biosensor design and fabrication. Biosensors used in environmental monitoring measure the toxicity effect based on the detection of a chemical compound or compound group by selective recognition of a biomolecule in the receptor layer and then by detection of a signal after passing through the transducer layer [16].The application of fluorescence biosensors in environmental protection applications [20][21][22][23][24][25], medical diagnostics [26][27][28][29][30][31][32][33][34][35], and industries [36][37][38][39][40][41][42][43][44][45] also is growing.Pol. J. Environ. Stud. Vol. 24, No. 1 (2015) AbstractEnzyme-based fluorescence biosensors and their applications in environmental protection, medicine, and industry are described. Biosensors used in environmental protection measure toxicity effects. A chemical compound or group of compounds is detected by the recognition of molecules in the receptor layer and then by detecting a signal passing through the transducer layer. Biosensors are classified according to the transduction method. Special emphasis is placed on optical biosensors, especially fluorescent biosensors, and such measurement techniques as FRET (Fröster resonance energy transfer), FLIM (fluorescence lifetime imaging), FCS (fluorescence correlation spectroscopy), and changes in fluorescence intensity. The phenomenon of fluorescence in biosensors and the selection of appropriate methods are described. The use of enzymes in the receptor layer and enzyme classification according to its category and functions used for analyte detection are presented. The fluorescence properties of enzymes resulting from possessing such cofactors as flavin or heme (prosthetic) groups are discussed. Several methods for enzyme immobilization, namely entrapment, adsorption, covalent immobilization, cross linking, and affinity interaction are described, and the use of enzymatic fluorescence biosensors in the detection of analytes is presented.

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“…These reviews are either presented through the prism of the technology involved or through the prism of the application domains addressed. Technologically speaking, one can mention biosensors based on electrochemical methods [1], Surface Plasmon Resonance (SPR), fluorescence, electro or chemiluminescence [2]. Ion Sensitive Field Effect Transistors (ISFET) or Enzyme Field Effect Transistors (EnFET) are the subject of a large amount of publications and several ISFET/ EnFET configurations are described [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Medical Devices Development: The Bottom-Up or The Top-Down Approach?

Wacogne¹

2018

IJBSBE

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Biomedical sensors are often developed under a bottom-up approach, i.e. the researcher point of view or laboratory approach. This is usually the case for microfluidic based integrated systems. The small number of such devices actually translated for use in clinical situations is mostly due to the need of sample pretreatment by specially trained people and to the fact that industrial transfer had not been taken into account since the very beginning of the development. Conversely, the top-down approach places the end-user at the center of development discussion. In this end-user point of view approach, the participation of industrial, medical and end-users partners more often leads to the design of fully integrated and automated devices which, furthermore, can be manufactured using conventional industrial capabilities. In this opinion communication, we briefly summarize the most common biosensors technologies and we explain how immuno-combined devices may help addressing constraints related to their use in clinical situations in terms of usability by non-trained people, automation and integration.

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Recent advances in biosensor based endotoxin detection

Cited by 135 publications

References 97 publications

Label free Affinity sensing: application to food analysis

Label free Affinity sensing: application to food analysis

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

Medical Devices Development: The Bottom-Up or The Top-Down Approach?

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