Recent advances in breeding citrus for drought and saline stress tolerance

Ben–Hayyim, Gozal; Moore, Gloria A.

doi:10.1007/978-1-4020-5578-2_25

Cited by 17 publications

(15 citation statements)

References 66 publications

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“…Other characters of interest have been tagged to QTLs, such as salinity tolerance (Ben-Hayyim and Moore, 2000) and nematode resistance (Ling et al, 2000). However, for citrus scion breeding programs, only the Alternaria resistance presented in this paper, along with the anthocyanin content, are currently subjected to MAS.…”

Section: Discussionmentioning

confidence: 99%

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

et al. 2016

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Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance genes and develops new molecular markers for ABS-resistance effective marker-assisted selection (MAS). Markers closely linked to ABSr locus were used for fine mapping using a 268-segregating diploid progeny derived from a heterozygous susceptible × resistant cross. Fine mapping limited the genomic region containing the ABSr resistance gene to 366 kb, flanked by markers at 0.4 and 0.7 cM. This region contains nine genes related to pathogen resistance. Among them, eight are resistance (R) gene homologs, with two of them harboring a serine/threonine protein kinase domain. These two genes along with a gene encoding a S-adenosyl-L-methionine-dependent-methyltransferase protein, should be considered as strong candidates for ABS-resistance. Moreover, the closest SNP was genotyped in 40 citrus varieties, revealing very high association with the resistant/susceptible phenotype. This new marker is currently used in our citrus breeding program for ABS-resistant parent and cultivar selection, at diploid, triploid and tetraploid level.

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Section: Discussionmentioning

confidence: 99%

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

et al. 2016

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“…Citrus is the most economically important fruit crop in the world. However, the productivity and fruit quality are adversely affected by drought and salinity stress 21 . Thus, improvement of tolerance to these two stresses can reduce economic loss to citrus growers.…”

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confidence: 99%

Combined analysis of mRNA and miRNA identifies dehydration and salinity responsive key molecular players in citrus roots

Xie

Zhang

et al. 2017

Sci Rep

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Citrus is one of the most economically important fruit crops around world. Drought and salinity stresses adversely affected its productivity and fruit quality. However, the genetic regulatory networks and signaling pathways involved in drought and salinity remain to be elucidated. With RNA-seq and sRNA-seq, an integrative analysis of miRNA and mRNA expression profiling and their regulatory networks were conducted using citrus roots subjected to dehydration and salt treatment. Differentially expressed (DE) mRNA and miRNA profiles were obtained according to fold change analysis and the relationships between miRNAs and target mRNAs were found to be coherent and incoherent in the regulatory networks. GO enrichment analysis revealed that some crucial biological processes related to signal transduction (e.g. ‘MAPK cascade’), hormone-mediated signaling pathways (e.g. abscisic acid- activated signaling pathway’), reactive oxygen species (ROS) metabolic process (e.g. ‘hydrogen peroxide catabolic process’) and transcription factors (e.g., ‘MYB, ZFP and bZIP’) were involved in dehydration and/or salt treatment. The molecular players in response to dehydration and salt treatment were partially overlapping. Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis further confirmed the results from RNA-seq and sRNA-seq analysis. This study provides new insights into the molecular mechanisms how citrus roots respond to dehydration and salt treatment.

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“…Reduction of tree size without affecting production or plant health is a desirable characteristic (Soost and Roose, 1996). Citrus are considered sensitive to salinity, though some species show same tolerance to salt concentration (Storey and Walker, 1999;Ben-Hayyim and Moore, 2007). Rootstock's benefits except salinity resistance, are higher-yield and better growth, higher photosynthesis, water content and elevated concentrations of antioxidants and abscisic acid and lower contents of sodium or chloride, compared to ungrafted plants Penella et al, 2016).…”

Section: Micropropagationmentioning

confidence: 99%

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

Salis

Papadakis

Kintzios

et al. 2017

Not Bot Horti Agrobo

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The behavior of six citrus rootstocks, Volkameriana, Citrumelo 'Swingle', Citrange 'Carrizo', Poncirus trifoliata 'Serra', Poncirus trifoliata 'Rubidoux' and Poncirus trifoliata 'Flying Dragon', in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock 'Swingle' showed a higher number of roots per explant (4.2) followed by 'Flying Dragon' (3.93) and 'Carrizo' (3.23) rootstocks. The rootstocks 'Swingle' (140.8 mm), Volkameriana (148 mm) and 'Flying Dragon' (131.12 mm) had significantly higher root length per explant compared to 'Carrizo' (31 mm) and 'Rubidoux' (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata 'Serra' and Citrumelo 'Swingle') and 0.60 (Volkameriana and Citrumelo 'Swingle'). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

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Recent advances in breeding citrus for drought and saline stress tolerance

Cited by 17 publications

References 66 publications

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Combined analysis of mRNA and miRNA identifies dehydration and salinity responsive key molecular players in citrus roots

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

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