Recent Advances in Cell Electrospining of Natural and Synthetic Nanofibers for Regenerative Medicine

Zamani, Reza; Aval, Sedigheh Fekri; Pilehvar-Soltanahmadi, Younes; Nejati-Koshki, Kazem; Zarghami, Nosratollah

doi:10.1055/s-0043-125314

Cited by 43 publications

(27 citation statements)

References 95 publications

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“…The set-up of a typical electrospinning apparatus mainly consists of a spinneret (e.g., a medical injector with a blunt tip), a boost pump for controlling the extrusion rate of a polymer solution, a direct-current electric field, and a grounded collector [2]. The electrospinning technique applies electrostatic principles to fabricate electrospun nanofibers [3]. Generally, in the electrospinning process, a polymer solution generates a cone-shaped drop beneath the needle under a strong electric field; then, the polymer drops overcome the surface tension to eject polymer nanofibers into the low electric field [4,5].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Nanofibers for Tissue Engineering with Drug Loading and Release

et al. 2019

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Electrospinning technologies have been applied in the field of tissue engineering as materials, with nanoscale-structures and high porosity, can be easily prepared via this method to bio-mimic the natural extracellular matrix (ECM). Tissue engineering aims to fabricate functional biomaterials for the repairment and regeneration of defective tissue. In addition to the structural simulation for accelerating the repair process and achieving a high-quality regeneration, the combination of biomaterials and bioactive molecules is required for an ideal tissue-engineering scaffold. Due to the diversity in materials and method selection for electrospinning, a great flexibility in drug delivery systems can be achieved. Various drugs including antibiotic agents, vitamins, peptides, and proteins can be incorporated into electrospun scaffolds using different electrospinning techniques and drug-loading methods. This is a review of recent research on electrospun nanofibrous scaffolds for tissue-engineering applications, the development of preparation methods, and the delivery of various bioactive molecules. These studies are based on the fabrication of electrospun biomaterials for the repair of blood vessels, nerve tissues, cartilage, bone defects, and the treatment of aneurysms and skin wounds, as well as their applications related to oral mucosa and dental fields. In these studies, due to the optimal selection of drugs and loading methods based on electrospinning, in vitro and in vivo experiments demonstrated that these scaffolds exhibited desirable effects for the repair and treatment of damaged tissue and, thus, have excellent potential for clinical application.

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Section: Introductionmentioning

confidence: 99%

Electrospun Nanofibers for Tissue Engineering with Drug Loading and Release

et al. 2019

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“…As in vivo cellular microenvironments are mainly composed of collagen nanofibrils, 26 several research groups have attempted to fabricate scaffolds composed of nanofibers. 27 Among various nanofiber fabrication techniques, electrospinning is considered a simple and versatile tool for producing nanofiber scaffolds for tissue engineering because of its ability to mimic the structure of the native extracellular matrix (ECM). 26,28,29 In addition, the nanofibers have the potential to increase cell adhesion by providing a wider surface area and improving the cell–material and cell–cell interaction.…”

Section: Introductionmentioning

confidence: 99%

Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation

Park

Lee

et al. 2019

J Tissue Eng

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The endothelialization on the poly (ε-caprolactone) nanofiber has been limited due to its low hydrophilicity. The aim of this study was to immobilize collagen on an ultra-thin poly (ε-caprolactone) nanofiber membrane without altering the nanofiber structure and maintaining the endothelial cell homeostasis on it. We immobilized collagen on the poly (ε-caprolactone) nanofiber using hydrolysis by NaOH treatment and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/sulfo- N-hydroxysulfosuccinimide reaction as a cost-effective and stable approach. NaOH was first applied to render the poly (ε-caprolactone) nanofiber hydrophilic. Subsequently, collagen was immobilized on the surface of the poly (ε-caprolactone) nanofibers using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/sulfo- N-hydroxysulfosuccinimide. Scanning electron microscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and fluorescence microscopy were used to verify stable collagen immobilization on the surface of the poly (ε-caprolactone) nanofibers and the maintenance of the original structure of poly (ε-caprolactone) nanofibers. Furthermore, human endothelial cells were cultured on the collagen-immobilized poly (ε-caprolactone) nanofiber membrane and expressed tight junction proteins with the increase in transendothelial electrical resistance, which demonstrated the maintenance of the endothelial cell homeostasis on the collagen-immobilized-poly (ε-caprolactone) nanofiber membrane. Thus, we expected that this process would be promising for maintaining cell homeostasis on the ultra-thin poly (ε-caprolactone) nanofiber scaffolds.

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“…Nanofiber scaffolds were developed in order to create a system similar to the natural extracellular matrix. Cells that dwell in tissue are inserted inside a 3D matrix composed of ambiguous collagen content [ 67 ].…”

Section: Scaffolds and Spheroidsmentioning

confidence: 99%

Adult Stem Cells Spheroids to Optimize Cell Colonization in Scaffolds for Cartilage and Bone Tissue Engineering

Baptista

Kronemberger

Côrtes

et al. 2018

IJMS

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Top-down tissue engineering aims to produce functional tissues using biomaterials as scaffolds, thus providing cues for cell proliferation and differentiation. Conversely, the bottom-up approach aims to precondition cells to form modular tissues units (building-blocks) represented by spheroids. In spheroid culture, adult stem cells are responsible for their extracellular matrix synthesis, re-creating structures at the tissue level. Spheroids from adult stem cells can be considered as organoids, since stem cells recapitulate differentiation pathways and also represent a promising approach for identifying new molecular targets (biomarkers) for diagnosis and therapy. Currently, spheroids can be used for scaffold-free (developmental engineering) or scaffold-based approaches. The scaffold promotes better spatial organization of individual spheroids and provides a defined geometry for their 3D assembly in larger and complex tissues. Furthermore, spheroids exhibit potent angiogenic and vasculogenic capacity and serve as efficient vascularization units in porous scaffolds for bone tissue engineering. An automated combinatorial approach that integrates spheroids into scaffolds is starting to be investigated for macro-scale tissue biofabrication.

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Recent Advances in Cell Electrospining of Natural and Synthetic Nanofibers for Regenerative Medicine

Cited by 43 publications

References 95 publications

Electrospun Nanofibers for Tissue Engineering with Drug Loading and Release

Electrospun Nanofibers for Tissue Engineering with Drug Loading and Release

Collagen immobilization on ultra-thin nanofiber membrane to promote in vitro endothelial monolayer formation

Adult Stem Cells Spheroids to Optimize Cell Colonization in Scaffolds for Cartilage and Bone Tissue Engineering

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