Recent advances in cucumber (<i>Cucumis sativus</i>L.)

Kaur, Manpreet; Sharma, Parul

doi:10.1080/14620316.2021.1945956

Cited by 17 publications

(9 citation statements)

References 195 publications

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“…GM papaya and alfalfa are also widely used in some countries [15]. Due to the substantial advantages of GM technology, it has also been successfully applied in vegetable and fruit research [16,17]. Bananas, being important fruits and food crops, have faced significant challenges due to the devastating impact of wilt disease, which has limited their yield and quality.…”

Section: Discussionmentioning

confidence: 99%

The Identification of the Banana Endogenous Reference Gene MaSPS1 and the Construction of Qualitative and Quantitative PCR Detection Methods

Zhu,

Lin,

Yang

et al. 2023

Genes

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Endogenous reference genes play a crucial role in the qualitative and quantitative PCR detection of genetically modified crops. Currently, there are no systematic studies on the banana endogenous reference gene. In this study, the MaSPS1 gene was identified as a candidate gene through bioinformatics analysis. The conservation of this gene in different genotypes of banana was tested using PCR, and its specificity in various crops and fruits was also examined. Southern blot analysis showed that there is only one copy of MaSPS1 in banana. The limit of detection (LOD) test showed that the LOD of the conventional PCR method is approximately 20 copies. The real-time fluorescence quantitative PCR (qPCR) method also exhibited high specificity, with a LOD of approximately 10 copies. The standard curve of the qPCR method met the quantitative requirements, with a limit of quantification (LOQ) of 1.14 × 10−2 ng—about 20 copies. Also, the qPCR method demonstrated good repeatability and stability. Hence, the above results indicate that the detection method established in this study has strong specificity, a low detection limit, and good stability. It provides a reliable qualitative and quantitative detection system for banana.

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Section: Discussionmentioning

confidence: 99%

The Identification of the Banana Endogenous Reference Gene MaSPS1 and the Construction of Qualitative and Quantitative PCR Detection Methods

Zhu,

Lin,

Yang

et al. 2023

Genes

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“…The success of these approaches in rapidly developing new cultivars to meet the demands of a growing population is yet to be fully realized. The improvement of various traits such as sex expression, disease resistance, and quality through these novel approaches is still an ongoing effort (Kaur and Sharma, 2021).…”

Section: Challenges and Limitationsmentioning

confidence: 99%

Genomic Advances in Cucurbitaceae: Implications for Crop Improvement and Breeding

Xu,

Yang,

Chen

2024

ijh

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The Cucurbitaceae family, encompassing a wide array of economically and nutritionally significant crops, has been the focus of extensive genomic research aimed at enhancing breeding and crop improvement. Recent advancements in sequencing technologies and bioinformatics have led to the sequencing of genomes from various Cucurbitaceae species, providing valuable insights into gene identification, genome evolution, and genetic variation. This has opened new avenues for molecular breeding, leveraging genetic transformation and gene editing technologies, including CRISPR/Cas9, to overcome the limitations of conventional breeding methods. The integration of next-generation sequencing (NGS) and omics approaches has furthered our understanding of complex traits, such as disease resistance and fruit quality, and has facilitated the development of high-density genetic maps and the identification of functional genes. Additionally, the construction of genetic and cytogenetic maps has been instrumental in revealing the genomic structure of cucurbit crops, aiding in the alignment of linkage groups with chromosomes and enhancing marker-assisted selection. The exploration of genetic diversity through the analysis of wild Cucurbitaceae species using cytogenetic mapping has also contributed to the phylogenetic understanding and breeding resource development. With the accumulation of genomic resources and the advent of high-throughput genotyping methods, new strategies such as genome-wide association studies (GWAS) and the use of multi-parent populations have emerged, leading to the discovery of quantitative trait loci (QTL) for key agronomic traits. The synergy of these genomic tools and their implications for breeding is poised to revolutionize the improvement of Cucurbitaceae crops, ensuring food security and meeting the demands of a growing population.

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“…Both biotic and abiotic stressful conditions cause severe yield loss and reduce quality of fruits (Das et al, 2022). Cucumber plant is sensitive to diverse biotic stresses like powdery mildew, downy mildew, different viruses and soil-borne disease, which lead to huge economic losses (Kaur and Sharma, 2022). Cucumber is reported as salt sensitive plant, which can tolerate salinity level about 2.5 dS m −1 , but its productivity reduces by 13% as a result for each increased unit of electrical conductivity over the threshold value (Chen et al, 2018; Al-Momany and Abu-Romman, 2023).…”

Section: Introductionmentioning

confidence: 99%

Utilizing of Different Cucurbit Rootstocks as a Sustainable Alternative to Enhance Growth, Productivity and Fruit Quality of Cucumber

Mohamed,

El-Tawashy

2024

Zagazig Journal of Agricultural Research

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Recent advances in cucumber (Cucumis sativusL.)

Cited by 17 publications

References 195 publications

The Identification of the Banana Endogenous Reference Gene MaSPS1 and the Construction of Qualitative and Quantitative PCR Detection Methods

The Identification of the Banana Endogenous Reference Gene MaSPS1 and the Construction of Qualitative and Quantitative PCR Detection Methods

Genomic Advances in Cucurbitaceae: Implications for Crop Improvement and Breeding

Utilizing of Different Cucurbit Rootstocks as a Sustainable Alternative to Enhance Growth, Productivity and Fruit Quality of Cucumber

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