Recent Advances in Detection of Plasmodium ovale: Implications of Separation into the Two Species Plasmodium ovale wallikeri and Plasmodium ovale curtisi

Fuehrer, Hans‐Peter; Noedl, Harald

doi:10.1128/jcm.02760-13

Cited by 50 publications

(48 citation statements)

References 27 publications

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“…Whereas nested PCR can detect the presence of the Plasmodium in level 6 parasites/ml of blood when the blood sample uses dried blood drops. This allows the PCR method to detect the presence of malaria parasites in a low level of parasitaemia and can also detect the presence of mixed infections which is often not detected by microscopic examination [6,8,9]. Although PCR has a good sensitivity and specificity, the routine use of nested PCR for the diagnosis of malaria cannot be done because of the complexity of the method, the high cost and it requires trained technicians and experts to conduct nested PCR method.…”

Section: Pcr Resultsmentioning

confidence: 99%

Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia

Arifin

Fitri

Sujuti

et al. 2018

J.Trop.Life.Science

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Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.

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Section: Pcr Resultsmentioning

confidence: 99%

Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia

Arifin

Fitri

Sujuti

et al. 2018

J.Trop.Life.Science

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show abstract

“…Research has shown that the target genes have highly conserved regions and their copy numbers range from 4 to 8. This feature makes these genes suitable for phylogenetic studies and molecular detection of different species of Plasmodium parasites [13]. Molecular technique is a very sensitive method of diagnosis of malaria parasite that can detect very low parasitemia level which the microscopy and the Rapid Diagnostic Tests cannot detect [14].…”

Section: Introductionmentioning

confidence: 99%

The effectiveness of microscopy: Rapid diagnostic test and molecular assay in diagnosing malaria.

Et¹,

Ia²,

Os³

2018

J Parasitic Diseases

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“…Recently, the world health organization (WHO) sets a mission on malaria elimination in West Asia and Southeast Asia in order to control this threat (2). Malaria is caused by eukaryotic microorganism from genus Plasmodium with 6 human-specific species including Plasmodium falciparum, P. vivax, P. malariae, P. knowlesi, P. ovale wallikeri, and P. ovale curtisi (3,4). Most of malaria cases in Iran are reported from Sistan and Baluchistan, Kerman, and Hormozgan provinces (5).…”

Section: Introductionmentioning

confidence: 99%

The Incidence of Current Infection with Different Human Malaria Species by Polymerase Chain Reaction for Diagnosis of Suspicious Malaria Patients on Elimination Region Sistan and Baluchistan Province, Southeast of Iran

Ebrahimzadeh

Dalir

Mehravaran

et al. 2017

Jundishapur J Microbiol

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Background: Malaria, as a parasitic disease, is one of the most important public health problems in Iran. Malaria is mainly diagnosed by peripheral blood smear, stained by Giemsa; in Iran it is also diagnosed by blood smear that is highly depends on technician's skills and laboratory properties. Objectives: Correct diagnosis of malaria and identification of human malaria species in spite of the measures taken to eliminate the disease in Iran, have made the complete understanding of malaria epidemiology critical. Therefore, the current study aimed at investigating the epidemiology of 2 species of human Plasmodium in Sistan and Baluchistan province using polymerase chain reaction (PCR) method. Methods: The present descriptive study was conducted on 100 patients suspected to malaria infection who referred to health centers of Chabahar, Iranshahr, Nikshahr, and Sarbaz districts. DNAs were extracted from blood samples using the specific kit, and nested-PCR reaction was performed to identify the Plasmodium species according to NP-2013 protocol. Results: Molecular analysis was performed on 100 samples suspected of malaria; 84 negative and 16 positive samples were detected including 8 Plasmodium vivax, 2 P. falciparum, and 6 mixed infections (P. vivax and P. falciparum). No P. ovale or P. malariae was observed. Conclusions:The results showed that malaria had a decreasing trend in Sistan and Baluchistan province. Therefore, the malaria elimination program is applicable and attainable in this region as a goal.

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Recent Advances in Detection of Plasmodium ovale: Implications of Separation into the Two Species Plasmodium ovale wallikeri and Plasmodium ovale curtisi

Cited by 50 publications

References 27 publications

Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia

Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia

The effectiveness of microscopy: Rapid diagnostic test and molecular assay in diagnosing malaria.

The Incidence of Current Infection with Different Human Malaria Species by Polymerase Chain Reaction for Diagnosis of Suspicious Malaria Patients on Elimination Region Sistan and Baluchistan Province, Southeast of Iran

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