Recent Advances in Electrochemical Biosensors Based on Enzyme Inhibition for Clinical and Pharmaceutical Applications

Harrad, Loubna El; Bourais, Ilhame; Mohammadi, Hasna; Amine, Aziz

doi:10.3390/s18010164

Cited by 114 publications

(49 citation statements)

References 122 publications

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“…Biosensors are commonly classified in electrochemical, optical, piezoelectric, or magnetic, based on the signal transduction mechanism. These technologies have broad applications in health (Zhang et al, 2017 ; El Harrad et al, 2018 ), food (Law et al, 2014 ; Vasilescu and Marty, 2016 ), and environmental sciences (Rapini and Marrazza, 2017 ; Kumar et al, 2018 ). Over the past years, the critical role of inflammation in disease has led researchers to develop biosensors for the specific detection of inflammatory mediators in clinically relevant body fluids.…”

Section: Sensing Circulating Biomarkers Of Neurodegeneration and Neurmentioning

confidence: 99%

Emerging Biosensing Technologies for Neuroinflammatory and Neurodegenerative Disease Diagnostics

Abreu

Soares-dos-Reis

Melo

et al. 2018

Front. Mol. Neurosci.

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Neuroinflammation plays a critical role in the onset and progression of many neurological disorders, including Multiple Sclerosis, Alzheimer's and Parkinson's diseases. In these clinical conditions the underlying neuroinflammatory processes are significantly heterogeneous. Nevertheless, a common link is the chronic activation of innate immune responses and imbalanced secretion of pro and anti-inflammatory mediators. In light of this, the discovery of robust biomarkers is crucial for screening, early diagnosis, and monitoring of neurological diseases. However, the difficulty to investigate biochemical processes directly in the central nervous system (CNS) is challenging. In recent years, biomarkers of CNS inflammatory responses have been identified in different body fluids, such as blood, cerebrospinal fluid, and tears. In addition, progress in micro and nanotechnology has enabled the development of biosensing platforms capable of detecting in real-time, multiple biomarkers in clinically relevant samples. Biosensing technologies are approaching maturity where they will become deployed in community settings, at which point screening programs and personalized medicine will become a reality. In this multidisciplinary review, our goal is to highlight both clinical and recent technological advances toward the development of multiplex-based solutions for effective neuroinflammatory and neurodegenerative disease diagnostics and monitoring.

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Section: Sensing Circulating Biomarkers Of Neurodegeneration and Neurmentioning

confidence: 99%

Emerging Biosensing Technologies for Neuroinflammatory and Neurodegenerative Disease Diagnostics

Abreu

Soares-dos-Reis

Melo

et al. 2018

Front. Mol. Neurosci.

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“…Nanomaterials are a popular carrier because of their large surface area [8,9]. At present, the application of enzyme electrodes based on nanomaterials has been widely explored [10]. The most common nanomaterials are noble metal nanoparticles, carbon materials (carbon nanotubes, graphene, carbon felt, etc.)…”

Section: Introductionmentioning

confidence: 99%

3D Hydrogen Titanate Nanotubes on Ti Foil: A Carrier for Enzymatic Glucose Biosensor

Yue

Huo

et al. 2020

Sensors

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Glucose oxidase (GOx) based biosensors are commercialized and marketed for the high selectivity of GOx. Incorporation nanomaterials with GOx can increase the sensitivity performance. In this work, an enzyme glucose biosensor based on nanotubes was fabricated. By using Ti foil as a carrier, hydrogen titanate nanotubes (HTNTs), which present fine 3D structure with vast pores, were fabricated in-situ by the hydrothermal treatment. The multilayer nanotubes are open-ended with a diameter of 10 nm. Then glucose oxidase (GOx) was loaded on the nanotubes by cross-linking to form an electrode of the amperometric glucose biosensor (GOx/HTNTs/Ti electrode). The fabricated GOx/HTNTs/Ti electrode had a linear response to 1–10 mM glucose, and the response time was 1.5 s. The sensitivity of the biosensor was 1.541 μA·mM-1·cm-2, and the detection limit (S/N = 3) was 59 μM. Obtained results indicate that the in-situ fabrication and unique 3D structure of GOx/HTNTs/Ti electrode are beneficial for its sensitivity.

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“…The use of AChE biosensors for the accurate identification and quantification of AChE inhibitors has been increased because they are sensitive, fast, cheap and portable they also allowed longer duration of enzyme activity, and multiple assays can be realized with eventual enzyme reactivation [8]. Furthermore, this biosensor can be used even in the detection of drugs for neurodegenerative diseases, particularly, Alzheimer's disease [9,10]. Electrochemical biosensors are powerful tools for the detection of OPs in real time, especially amperometric biosensors [11,12].…”

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

Khaldi¹,

Sam²,

Gabouze³

2020

I2M

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An amperometric method to measure the total amount of active and inhibited immobilized Acetylcholinesterase enzyme (AChE) and the quantification of AChE inhibitors was realized. In this approach porous silicon (PSi) surface was used as a matrix for AChE immobilization and Boron doped polycrystalline diamant (BDPD) was used as working electrode for the electrochemical measurements. The covalent immobilization of AChE from electric eel was achieved on amine functionalized PSi surface previously decorated with Au particles. This surface is suitable for a stable attachment of AChE enzyme. The amperometric detection of AChE activity at Boron doped polycrystalline diamond electrode is based on the oxidation of thiocholine, the enzymatic reaction product of immobilized AChE in the presence of the substrate acetylthiocholine chlorid (ATCl) and this without the need to further modify the BDPD surface or the use of other reagents. The concentration of immobilized active AChE enzymes was estimated to Γ≈ 1.8 10 12 AChE cm -2 by means of a calibration curve. Michaelis constant was assessed with a Km of 4.3 10 -4 M. Finally, the electrochemical quantification and detection of Malathion shows a good linear calibration curve where the concentration of Malathion range is from 2 to 6 nM within 6 minutes after inhibitor addition.

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Recent Advances in Electrochemical Biosensors Based on Enzyme Inhibition for Clinical and Pharmaceutical Applications

Cited by 114 publications

References 122 publications

Emerging Biosensing Technologies for Neuroinflammatory and Neurodegenerative Disease Diagnostics

Emerging Biosensing Technologies for Neuroinflammatory and Neurodegenerative Disease Diagnostics

3D Hydrogen Titanate Nanotubes on Ti Foil: A Carrier for Enzymatic Glucose Biosensor

Acetylcholinesterase Modified Porous Silicon for Electrochemical Measurement of Total Active Immobilized Enzyme Amount and Effective Malathion Detection

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