Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay

Jin, Longsheng; Liu, Weishuai; Yang, Haijian; Yu, Huihui; Dong, Chunyan; Wu, Mei-Sheng

doi:10.3390/bios13060653

Cited by 6 publications

(1 citation statement)

References 104 publications

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“…Particularly, those with electrochemical detection provide excellent sensitivity, selectivity, and rapid analysis without complex pretreatment procedures, making them advantageous for fast and straightforward determination methods. Additionally, these systems enable real-time and portable analysis, making them well suited for precise mycotoxin quantification in remote locations or field analysis scenarios [25,26].…”

Section: Introductionmentioning

confidence: 99%

Origami Paper-Based Electrochemical Immunosensor with Carbon Nanohorns-Decorated Nanoporous Gold for Zearalenone Detection

Laza,

Pereira,

Messina

et al. 2024

Chemosensors

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Nowadays, mycotoxin contamination in cereals and wastewater exposes a safety hazard to consumer health. This work describes the design of a simple, low-cost, and sensitive origami microfluidic paper-based device using electrochemical detection for zearalenone determination. The microfluidic immunosensor was designed on a paper platform by a wax printing process. The graphitized carbon working electrode modified with carbon nanohorns-decorated nanoporous gold showed a higher surface area, sensitivity, and adequate analytical performance. Electrodes were characterized by scanning electron microscopy, energy-dispersive spectroscopy, and cyclic voltammetry. The determination of zearalenone was carried out through a competitive immunoassay using specific antibodies immobilized by a covalent bond on the electrode surface. In the presence of HRP-labeled enzyme conjugate, substrate, and catechol, zearalenone was detected employing the developed immunosensor by applying −0.1 V to the working electrode vs silver as a pseudo-reference electrode. A calibration curve with a linear range between 10 and 1000 µg Kg−1 (R2 = 0.998) was obtained, and the limit of detection and quantification for the electrochemical immunosensor were 4.40 and 14.90 µg Kg−1, respectively. The coefficient of variation for intra- and inter-day assays was less than 5%. The selectivity and specificity of the sensor were evaluated, comparing the response against zearalenone metabolites and other mycotoxins that could affect the corn samples. Therefore, origami is a promising approach for paper-based electrochemical microfluidic sensors coupled to smartphones as a rapid and portable tool for in situ mycotoxins detection in real samples.

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Section: Introductionmentioning

confidence: 99%