Recent Advances in Engineering Carriers for siRNA Delivery

Yang, Chengbin; Lin, Zheng‐Ian; Zhang, Xinmeng; Xu, Zhourui; Xu, Gaixia; Wang, Yu‐Min; Tsai, Tzu‐Hsien; Cheng, Pei‐Wen; Law, Wing‐Cheung; Yong, Ken‐Tye; Chen, Chih‐Kuang

doi:10.1002/mabi.202300362

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2024

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Cited by 3 publications

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Molecular level design of conjugated polymers based on a synergistic adsorption-photocatalytic strategy for efficient uranium capture

Liang,

Li,

et al. 2024

Chemical Engineering Journal

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Molecular level design of conjugated polymers based on a synergistic adsorption-photocatalytic strategy for efficient uranium capture

Liang,

Li,

et al. 2024

Chemical Engineering Journal

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RNA-binding peptide and endosomal escape-assisting peptide (L2) improved siRNA delivery by the hexahistidine–metal assembly

Zhang,

Qin,

Feng

et al. 2024

J. Mater. Chem. B

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Role of size, surface charge, and PEGylated lipids of lipid nanoparticles (LNPs) on intramuscular delivery of mRNA

Kong,

Wei,

Dong

et al. 2024

Preprint

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Background Lipid nanoparticles (LNPs) are currently the most commonly used non-viral gene delivery system. Their physiochemical attributes, encompassing size, charge and surface modifications, significantly affect their behaviors both in vivo and in vitro. Nevertheless, the effects of these properties on the transfection and distribution of LNPs after intramuscular injection remain elusive. In this study, LNPs with varying sizes, lipid-based charges and PEGylated lipids were formulated to study their transfection and in vivo distribution. Luciferase mRNA (mLuc) was loaded in LNPs as a model nucleic acid. Results In vivo and in vitro results indicated that smaller-sized LNPs and those with neutral potential presented superior transfection efficiency after intramuscular injection. Surprisingly, the sizes and charges did not exert a notable influence on the in vivo distribution of the LNPs. Furthermore, PEGylated lipids with shorter acyl chains contributed to enhanced transfection efficiency due to their superior cellular uptake and lysosomal escape capabilities. Notably, the mechanisms underlying cellular uptake differed among LNPs containing various types of PEGylated lipids, which was primarily attributed to the length of their acyl chain. Conclusions Together, these insights underscore the pivotal role of nanoparticle characteristics and PEGylated lipids in the intramuscular route. This study not only fills crucial knowledge gaps but also provides invaluable directions for the effective delivery of mRNA via LNPs.

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Recent Advances in Engineering Carriers for siRNA Delivery

Cited by 3 publications

References 408 publications

Molecular level design of conjugated polymers based on a synergistic adsorption-photocatalytic strategy for efficient uranium capture

Molecular level design of conjugated polymers based on a synergistic adsorption-photocatalytic strategy for efficient uranium capture

RNA-binding peptide and endosomal escape-assisting peptide (L2) improved siRNA delivery by the hexahistidine–metal assembly

Role of size, surface charge, and PEGylated lipids of lipid nanoparticles (LNPs) on intramuscular delivery of mRNA

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