Recent advances in live cell imaging of hepatoma cells

Salipalli, Sandeep; Singh, Prafull; Borlak, Jürgen

doi:10.1186/1471-2121-15-26

Cited by 11 publications

(6 citation statements)

References 110 publications

(130 reference statements)

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“…Fluorescence imaging has emerged as one of the most powerful techniques to monitor targets and biological processes in the context of a living system with high temporal and spatial resolution. − Luminescence imaging and cytotoxicity studies of all the compounds were carried out in HeLa cells. Cytotoxicity studies on these compounds showed that 3 and 4 were less cytotoxic as compared with 1 and 2 (Figure S66).…”

Section: Results and Discussionmentioning

confidence: 99%

Delayed Fluorescence, Room Temperature Phosphorescence, and Mechanofluorochromic Naphthalimides: Differential Imaging of Normoxia and Hypoxia Live Cancer Cells

et al. 2020

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We study the effect of molecular conformation on the electronic coupling between the donor amines and acceptor 1,8-naphthalimide (NPI) in a series of D–A systems 1–4 (A = NPI; D = phenothiazine, phenoxazine, carbazole, diphenylamine, respectively, for 1, 2, 3, and 4). Weakly coupled systems show dual emission in the solution state, while strongly coupled systems show single emission bands. The energy of transitions and photoluminescence (PL) quantum yield are sensitive to the molecular conformation and donor strength. These compounds show delayed emission in the solutions and aggregated state and phosphorescence in the solid state. Compounds 3 and 4 with weak donors exhibit intermolecular slipped π···π interactions in the solid state and consequently exhibit dual (intra- and inter-) phosphorescence at low temperature. Steady state and time-resolved PL studies at variable temperature together with computational and crystal structure analysis were used to rationalize the optical properties of these compounds. The delayed emission of these compounds is sensitive to molecular oxygen; accordingly, these molecules are utilized for differential imaging of normoxia and hypoxia cancer cells.

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Section: Results and Discussionmentioning

confidence: 99%

Delayed Fluorescence, Room Temperature Phosphorescence, and Mechanofluorochromic Naphthalimides: Differential Imaging of Normoxia and Hypoxia Live Cancer Cells

et al. 2020

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“…Therefore, optical imaging, such as fluorescence and bioluminescence imaging, offers the promise of accurate tumor diagnosis through noninvasive, real-time, and high-resolution imaging. Fluorescence bioimaging as a technique to visualize specific organelles in live cells [5][6][7][8][9][10] and whole animals 11,12 has become a powerful supporting tool for biological research, [13][14][15][16] and even for clinical utilization such as in the emerging field of fluorescence-guided surgery. [17][18][19][20][21] Additionally, fluorescence bioimaging can capture specific molecular information on tumor structure and tumor metabolism.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy

et al. 2017

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Surgical resection of solid tumors is currently the gold standard and preferred therapeutic strategy for cancer. Chemotherapy drugs also make a significant contribution by inhibiting the rapid growth of tumor cells and these two approaches are often combined to enhance treatment efficacy. However, surgery and chemotherapy inevitably lead to severe side effects and high systemic toxicity, which in turn results in poor prognosis. Precision medicine has promoted the development of treatment modalities that are developed to specifically target and kill tumor cells. Advances in in vivo medical imaging for visualizing tumor lesions can aid diagnosis, facilitate surgical resection, investigate therapeutic efficacy, and improve prognosis. In particular, the modality of fluorescence imaging has high specificity and sensitivity and has been utilized for medical imaging. Therefore, there are great opportunities for chemists and physicians to conceive, synthesize, and exploit new chemical probes that can image tumors and release chemotherapy drugs in vivo. This review focuses on small molecular ligand-targeted fluorescent imaging probes and fluorescent theranostics, including their design strategies and applications in clinical tumor treatment. The progress in chemical probes described here suggests that fluorescence imaging is a vital and rapidly developing field for interventional surgical imaging, as well as tumor diagnosis and therapy.

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“…Ehrlich's use of synthetic dyes as a means of staining biological samples can be viewed as one of the foundation stones of modern scientific research. A century later, the use of fluorescence imaging as a technique to visualize specific regions of live cellular 1 2 3 4 or whole organisms 5 6 is often central to research programmes, with clinical applications such as fluorescence-guided surgery now emerging 7 9 10 11 .…”

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confidence: 99%

Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time

et al. 2016

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Bioresponsive NIR-fluorophores offer the possibility for continual visualization of dynamic cellular processes with added potential for direct translation to in vivo imaging. Here we show the design, synthesis and lysosome-responsive emission properties of a new NIR fluorophore. The NIR fluorescent probe design differs from typical amine functionalized lysosomotropic stains with off/on fluorescence switching controlled by a reversible phenol/phenolate interconversion. Emission from the probe is shown to be highly selective for the lysosomes in co-imaging experiments using a HeLa cell line expressing the lysosomal-associated membrane protein 1 fused to green fluorescent protein. The responsive probe is capable of real-time continuous imaging of fundamental cellular processes such as endocytosis, lysosomal trafficking and efflux in 3D and 4D. The advantage of the NIR emission allows for direct translation to in vivo tumour imaging, which is successfully demonstrated using an MDA-MB-231 subcutaneous tumour model. This bioresponsive NIR fluorophore offers significant potential for use in live cellular and in vivo imaging, for which currently there is a deficit of suitable molecular fluorescent tools.

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Recent advances in live cell imaging of hepatoma cells

Cited by 11 publications

References 110 publications

Delayed Fluorescence, Room Temperature Phosphorescence, and Mechanofluorochromic Naphthalimides: Differential Imaging of Normoxia and Hypoxia Live Cancer Cells

Delayed Fluorescence, Room Temperature Phosphorescence, and Mechanofluorochromic Naphthalimides: Differential Imaging of Normoxia and Hypoxia Live Cancer Cells

Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy

Lysosome triggered near-infrared fluorescence imaging of cellular trafficking processes in real time

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