Recent advances in microcontact printing

Quist, Arjan P.; Pavlovic, Elisabeth; Oscarsson, Sven

doi:10.1007/s00216-004-2847-z

Cited by 267 publications

(196 citation statements)

References 146 publications

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“…We then microstamped the coverslips with 150-to 200-m-diameter circular islands of collagen adsorbed onto a polydimethylsiloxane (PDMS) stamp. The microstamp was fabricated by molding PDMS on thick photoresist patterned using photolithography and microcontact printing techniques (Quist et al, 2005;Falconnet et al, 2006). In brief, SU-8 2015 photoresist (MicroChem, Newton, MA) was dispensed on a silicon wafer and spun into a thin film using a spin coater (WS-400B-6NPP-Lite; Laurell Technologies, North Wales, PA) at 3000 rpm for 1 min to produce a film thickness of ϳ15 m. Prebake of photoresist by heating to 95°C for 3 min on a hotplate was followed by exposure to UV light (US-KVB30D; Computronics Corporation, Bentley, Australia) through a 5080 dpi transparency photomask.…”

Section: Methodsmentioning

confidence: 99%

Vesicle Pool Heterogeneity at Hippocampal Glutamate and GABA Synapses

Moulder

Jiang²,

Taylor³

et al. 2007

J. Neurosci.

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Glutamate and GABA are the major fast excitatory and inhibitory neurotransmitters, respectively, in the CNS. Although glutamate and GABA have clearly distinct postsynaptic actions, we are just beginning to appreciate that presynaptic differences between glutamatergic and GABAergic neurons may contribute to distinct functions of these transmitter systems. We therefore probed possible differences between the functional synaptic vesicle populations of glutamatergic and GABAergic neurons. We examined superecliptic synaptopHluorin (SpH) fluorescence during 20 Hz electrical stimulation in transfected hippocampal neurons and identified the phenotype of SpH-fluorescent synapses with post hoc immunostaining. With 200 stimuli (10 s), individual glutamate synapses displayed considerably more variability in peak SpH fluorescence than GABA synapses, without a strong difference in the mean SpH fluorescence increase. This spatial heterogeneity could not be accounted for by differences in endocytosis, which was nearly constant over these short time periods across glutamate and GABA synapses. Instead, variability in vesicle exocytosis correlated with variability in total vesicle staining and in measures of the total recycling pool size. Differences were also evident using FM1-43 [N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] uptake. These data support the idea that the population of glutamate synapses exhibits more heterogeneity in release properties than the population of GABA synapses, possibly correlated with glutamatergic synaptic malleability.

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Section: Methodsmentioning

confidence: 99%

Vesicle Pool Heterogeneity at Hippocampal Glutamate and GABA Synapses

Moulder

Jiang²,

Taylor³

et al. 2007

J. Neurosci.

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“…1 Self-assembly methods 1,2 are generally limited to creation of uniform 2D films. 3,4 Printing methods, 5,6 which have the potential for organizing nanostructures through creation of chemical templates, can rarely achieve patterning below 50 nm, and then controlling orientations of individual nanostructures is difficult. One approach to achieving higher density of functionality and shorter range order is to use macromolecular complexes -such as virions -as programmable building blocks, either for synthesis of nanoparticles and nanowires or for precise placement of molecular moieties exhibiting optical and electronic functions.…”

Section: Introductionmentioning

confidence: 99%

Physical Controls on Directed Virus Assembly at Nanoscale Chemical Templates

et al. 2006

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Viruses are attractive building blocks for nanoscale heterostructures, but little is understood about the physical principles governing their directed assembly. In-situ force microscopy was used to investigate organization of Cowpea Mosaic Virus engineered to bind specifically and reversibly at nanoscale chemical templates with sub-30nm features. Morphological evolution and assembly kinetics were measured as virus flux and inter-viral potential were varied. The resulting morphologies were similar to those of atomic-scale epitaxial systems, but the underlying thermodynamics was analogous to that of colloidal systems in confined geometries. The 1D templates biased the location of initial cluster formation, introduced asymmetric sticking probabilities, and drove 1D and 2D condensation at subcritical volume fractions. The growth kinetics followed a t 1/2 law controlled by the slow diffusion of viruses. The lateral expansion of virus clusters that initially form on the 1D templates following introduction of polyethylene glycol (PEG) into the solution suggests a significant role for weak

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“…These non-lithographic techniques require minimal monetary investment (clean room not necessary), can be conducted under normal bench top laboratory conditions, and are conceptually simple to fabricate. Some of the diverse fabrication methods known collectively as soft lithography include: replica molding (Xia et al, 1997), micromolding in capillaries-MIMIC (Zhang et al, 2002), microcontact printing-μCP (Quist et al, 2005), and microtransfer molding-μTM (Zhao et al, 1996). Schematic illustrations of some these procedures are depicted in Fig.…”

Section: Soft Lithographymentioning

confidence: 99%