Recent advances in microfluidic devices for single-cell cultivation: methods and applications

Anggraini, Dian; Ota, Noriyasu; Shen, Yingjia; Tang, Tao; Tanaka, Yo; Hosokawa, Yoichiroh; Li, Ming; Yalikun, Yaxiaer

doi:10.1039/d1lc01030a

Cited by 32 publications

(29 citation statements)

References 233 publications

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“…[ 114 , 156 ] While in microchamber arrays, the reagents are transported to each microchamber through a single pipeline spanning. [ 157 ] Accurate quantification of digital microfluidics ensured that each target nucleic acid had the same probability of participating in the reaction system. Additionally, when integrated with CRISPR biosensing, this universal, quantifiable, and straightforward microfluidic device has captured increasing attention for infectious diseases diagnosis.…”

Section: Clustered Regularly Interspaced Short Palindromic Repeats‐ba...mentioning

confidence: 99%

Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Xie

Chen

et al. 2022

Advanced Science

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Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. Conventional diagnostic techniques for infectious diseases typically require sophisticated equipment and are time consuming. Emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) detection systems have shown remarkable potential as next-generation diagnostic tools to achieve rapid, sensitive, specific, and field-deployable diagnoses of infectious diseases, based on state-of-the-art microfluidic platforms. Therefore, a review of recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis is urgently required. This review highlights the mechanisms of CRISPR/Cas biosensing and cutting-edge microfluidic devices including paper, digital, and integrated wearable platforms. Strategies to simplify sample pretreatment, improve diagnostic performance, and achieve integrated detection are discussed. Current challenges and future perspectives contributing to the development of more effective CRISPR-based microfluidic diagnostic systems are also proposed.

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Section: Clustered Regularly Interspaced Short Palindromic Repeats‐ba...mentioning

confidence: 99%

Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Xie

Chen

et al. 2022

Advanced Science

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“…Unlike the microarray electrofusion chip, the microdroplet electrofusion chip utilizes two immiscible fluids, such as oil and water: one as a continuous phase and the other as a dispersed phase. By controlling the flow of the two phases using microfluidic technology, microdroplet generation, transport, fusion, and sorting can be achieved [23,24] . With the properties of monodispersion and picoliter-sized volume, the microdroplet provides a powerful platform for precise cell fusion.…”

Section: Microdroplet Electrofusion Chipmentioning

confidence: 99%

Advances in hybridoma preparation using electrofusion technology

Kou

Shen

wang

et al. 2022

Preprint

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As a rapidly developing cell engineering technique, cell electrofusion has been increasingly applied in the field of hybridoma preparation in recent years. However, electrofusion is a certain degree of difficulty to completely replace the polyethylene glycol-mediated cell fusion. The key elements limiting electrofusion in the field of hybridoma preparation are practical complicated. This review summarizes the state of art of cell electrofusion in hybridoma preparation based on recent published literatures, mainly focusing on electrofusion instruments and their components, process control, cell treatment, and process characterization. The review provides new information and insightful commentary, critically important to the promotion of further electrofusion development in the field of hybridoma preparation.

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“…Flow cytometry enables the improved understanding of cell functions, the measurement of biophysical information on individual cells, and the characterization of cell heterogeneity in a large population, − which provides important tools for fundamental biological research and clinical diagnosis . As a novel method for precise cell manipulation and detection, microfluidics provides new insights for realizing the high-throughput cell focusing, counting, and identification functions of conventional flow cytometers due to the offered advantages of low device cost, simple operation, low sample consumption, and parallel processing capacity. , In a microfluidic flow cytometer, the fluorescent signals, optical images, , and impedance changes , of cells could be measured in a high-throughput and continuous-flow manner when the cells flow through the detection region.…”

Section: Introductionmentioning

confidence: 99%

Controllable Size-Independent Three-Dimensional Inertial Focusing in High-Aspect-Ratio Asymmetric Serpentine Microchannels

Zhou

Zhu

et al. 2022

Anal. Chem.

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High-throughput three-dimensional (3D) focusing of cells is the key prerequisite for enabling accurate microfluidic cell detection and analysis. In this work, we develop a high-aspectratio asymmetric serpentine (HARAS) microchannel for single-line inertial focusing of particles and cells at the 3D center of the channel. The mechanism of 3D focusing is explored by numerical simulation, and the focusing performances of differently sized particles are characterized experimentally at different flow rates. The results demonstrate the outstanding 3D single-line focusing capability of our HARAS microchannel. In addition, the phenomena of size-independent and position-controllable focusing over wide flow rates are observed. Finally, the applicability of our HARAS microchannel for processing real biological cells is validated by the 3D single-line focusing of A549 cells and MCF-7 cells. Our work overcomes the issue of off-centered focusing of most previous works and provides new insights into the 3D focusing in inertial microfluidics. The proposed HARAS microchannel is extremely easy for mass production and may provide a stable, high-throughput, and position-controllable scheme for subsequent single-cell detection and analysis.

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Recent advances in microfluidic devices for single-cell cultivation: methods and applications

Abstract: Single-cell cultivation is essential to investigate the actual cellular and subcellular information of individual cells and enhance single-cell colonies and cell-derived products for further analysis at a single-cell level.

Cited by 32 publications

References 233 publications

Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Advances in hybridoma preparation using electrofusion technology

Controllable Size-Independent Three-Dimensional Inertial Focusing in High-Aspect-Ratio Asymmetric Serpentine Microchannels

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