Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Zhao, Yan; Su, Zhuoqun; Zhang, Xue; Wu, Di; Wu, Yongning; Li, Guoliang

doi:10.1039/d3ay02040a

Cited by 4 publications

(1 citation statement)

References 143 publications

(164 reference statements)

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“…Nanopore glycan analysis.-Nanopore glycan analysis has had a far more measured pace of development than was seen for nanopore DNA sequencing, 35,58 as has recently been critically reviewed. 60 A small collection of experiments over more than two decades has used different nanopore types with and without surface-bound carbohydrate capture agents (e.g. lectins and boronic acids) and chemical labelling of analytes to characterize a range of glycan samples and characteristics.…”

Section: Current Statusmentioning

confidence: 99%

Editors’ Choice—Perspective—Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science

Kizer,

R. Dwyer

2024

ECS Sens. Plus

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Glycans, or complex carbohydrates, are information-rich biopolymers critical to many biological processes and with considerable importance in pharmaceutical therapeutics. Our understanding, though, is limited compared to other biomolecules such as DNA and proteins. The greater complexity of glycan structure and the limitations of conventional chemical analysis methods hinder glycan studies. Auspiciously, nanopore single-molecule sensors—commercially available for DNA sequencing—hold great promise as a tool for enabling and advancing glycan analysis. We focus on two key areas to advance nanopore glycan characterization: molecular surface coatings to enhance nanopore performance including by molecular recognition, and high-quality glycan chemical standards for training.

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Section: Current Statusmentioning

confidence: 99%

Editors’ Choice—Perspective—Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science

Kizer,

R. Dwyer

2024

ECS Sens. Plus

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Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting

Jangid,

Kim

2024

Advances in Colloid and Interface Science

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Nanopore-Based Biomimetic ssDNA Reporters for Multiplexed Detection of Meat Adulteration

Su,

Zhang,

Wang

et al. 2024

J. Agric. Food Chem.

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Meat adulteration detection is crucial for ensuring food safety, protecting consumer rights, and maintaining market integrity. However, the current methods face challenges in achieving multiplexed detection through efficient signal conversion and output. This study introduces a nanopore-based approach for the simultaneous detection of multiple meat adulteration. Leveraging the interaction between D A D A dipeptide and vancomycin, we designed a series of biomimetic ssDNA reporters with D A D A-Van tags for multiplexed signal output. These ssDNA reporters can generate highly distinctive current blockage signals, which can be discriminated simultaneously by the current waveform. Combined with PCR-based strand displacement, ssDNA reporters can be released from magnetic beads in the presence of the target gene and are analyzed using α-hemolysin (α-HL) nanopores for multiplexed signal output. The signal frequency for each target gene has a linear relationship with the specific concentration range (for duck: 1 × 10 −3 ∼ 10 nmol/L, for pork: 1 × 10 −2 ∼ 10 nmol/L, and for chicken: 1 × 10 −3 ∼ 10 nmol/L). The limits of detection are as follows: 0.418 pmol/L for duck, 4.473 pmol/L for pork, and 0.531 pmol/L for chicken. This method effectively enables the simultaneous detection of adulterated chicken, pork, and duck meat in lamb samples and holds potential for broad applications in ensuring the authenticity and safety of meat products.

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Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Abstract: Saccharides are not only the basic constituents and nutrients of living organisms, but also participate in various life activities, and play important roles in cell recognition, immune regulation, development, cancer...

Cited by 4 publications

References 143 publications

Editors’ Choice—Perspective—Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science

Editors’ Choice—Perspective—Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science

Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting

Nanopore-Based Biomimetic ssDNA Reporters for Multiplexed Detection of Meat Adulteration

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