Recent advances in polymer scaffolds for biomedical applications

Sharma, Deepika; Saha, Sampa; Satapathy, Bhabani K.

doi:10.1080/09205063.2021.1989569

Cited by 24 publications

(7 citation statements)

References 338 publications

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“…The fiber samples were incubated in an 80 RPM shaker at 37 °C for 28 days. The supernatants were completely removed and stored at −80 °C, and an equal amount of fresh PBS (pH 7.4) was supplemented each time at 1,2,3,4,5,6,7,8,10,12,14,16,18,20,22,24,26, and 28 days. The accumulated amounts of drug released were quantified using an MCP-1 ELISA kit (Peprotech Nordic, Sweden).…”

Section: In Vitro Release Of Mcp-1 From Plcl/mcp-1 Fibrous Filmsmentioning

confidence: 99%

“…The commercially available synthetic vascular grafts fabricated from non-biodegradable polymers, such as expanded polytetrafluoroethylene (ePTFE) and polyethylene terephthalate (PET), have been clinically used for large-diameter vessels (>6 mm) . However, they are apt to fail in small-diameter vessels due to intimal hyperplasia, thrombosis formation, chronic foreign body reaction (FBR), mechanical mismatch, and a lack of functional endothelial coverage. ,, Small-diameter blood vessels remain a clinical challenge, and there is an urgent need for alternative solutions. , Recently, tissue engineering of small-diameter blood vessels has emerged as a promising solution with suitable mechanical properties and better biocompatibility. ,, As a promising tissue engineering approach, the electrospinning technique can fabricate nanofibrous scaffolds that have the potential to address these issues. ,− …”

Section: Introductionmentioning

confidence: 99%

“…7,14 Recently, tissue engineering of small-diameter blood vessels has emerged as a promising solution with suitable mechanical properties and better biocompatibility. 14,18,19 As a promising tissue engineering approach, the electrospinning technique can fabricate nanofibrous scaffolds that have the potential to address these issues. 14,20−22 Implanted nanofibrous scaffolds will stimulate the innate immune system, which elicits a series of immune responses that ultimately lead to FBR and fibrous encapsulation, leading to graft failure.…”

Section: Introductionmentioning

confidence: 99%

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MCP-1-Loaded Poly(l-lactide-co-caprolactone) Fibrous Films Modulate Macrophage Polarization toward an Anti-inflammatory Phenotype and Improve Angiogenesis

Cheng

et al. 2023

ACS Biomater. Sci. Eng.

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Tissue engineering approaches such as the electrospinning technique can fabricate nanofibrous scaffolds which are widely used for smalldiameter vascular grafting. However, foreign body reaction (FBR) and lack of endothelial coverage are still the main cause of graft failure after the implantation of nanofibrous scaffolds. Macrophage-targeting therapeutic strategies have the potential to address these issues. Here, we fabricate a monocyte chemotactic protein-1 (MCP-1)-loaded coaxial fibrous film with poly(L-lactide-co-ε-caprolactone) (PLCL/MCP-1). The PLCL/MCP-1 fibrous film can polarize macrophages toward anti-inflammatory M2 macrophages through the sustained release of MCP-1. Meanwhile, these specific functional polarization macrophages can mitigate FBR and promote angiogenesis during the remodeling of implanted fibrous films. These studies indicate that MCP-1-loaded PLCL fibers have a higher potential to modulate macrophage polarity, which provides a new strategy for small-diameter vascular graft designing.

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Section: In Vitro Release Of Mcp-1 From Plcl/mcp-1 Fibrous Filmsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MCP-1-Loaded Poly(l-lactide-co-caprolactone) Fibrous Films Modulate Macrophage Polarization toward an Anti-inflammatory Phenotype and Improve Angiogenesis

Cheng

et al. 2023

ACS Biomater. Sci. Eng.

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“…Fabrication techniques for polymer scaffolds include self-assembly, phase separation, and electrospinning [5][6][7][8] . Electrospinning is a highly versatile, cost-effective, and sustainable method for manufacturing scaffolds using a diverse range of polymer materials 9,10 . The process employs electrostatic forces to produce uniform bers, yielding structures that demonstrate a noteworthy degree of precision and uniformity 11 .…”

Section: Introductionmentioning

confidence: 99%

Enhancing Cell Growth with PAN/PVA-Gelatin 3D Scaffold: A Novel Approach using In-situ UV Radiation Electrospinning and Plasma Treatment

Khavari,

Jahanfar,

Anaghizi

et al. 2023

Preprint

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The hydrophobic nature of synthetic polymers poses a substantial barrier since it limits cell-seeding and proliferation scaffold performance. To overcome this challenge, the present research attempts to employ in-situ UV electrospinning and plasma surface modification techniques to fabricate a three-dimensional PAN/PVA-gelatin scaffold. The proposed scaffold holds great potential in mitigating hydrophobicity limitations, thereby facilitating enhanced cell adhesion and proliferation. The SEM results indicated that exposure to UV irradiation resulted in the formation of wavy shapes in the PAN microstructures and crosslinking between fibers within the scaffold. Moreover, plasma treatment induced the formation of pores on the PAN surface, with an average diameter of 43 µm, corresponding to the size range of mouse fibroblast cells. Furthermore, the plasma treatment provided roughness augmentation of the scaffold surface, which played a crucial role in enhancing cell adhesion and elongation on the modified scaffold surface. Comparatively, the plasma-modified scaffolds exhibited a higher proportion of viable cells than the unmodified scaffolds (p < 0.05). Moreover, the implementation of perforations in the PAN layer via plasma treatment reduced the number of necrosis cells in comparison to the other samples. In contrast, the unmodified scaffold showed a higher percentage of apoptosis cells (p < 0.05).

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“…This design approach is well-suited for enzyme immobilization. Currently, there are seven main 3D printing technologies: material extrusion (ME), vat photopolymerization (VP), powder bed fusion (PBF), material jetting (MJ), binder jetting (BJ), sheet lamination (SL), and directed energy deposition (DED) [ 16 , 17 , 18 , 19 ]. Among these technologies, ME and VP are the two most common methods used for enzyme immobilization.…”

Section: Introductionmentioning

confidence: 99%

Advances in 3D Gel Printing for Enzyme Immobilization

Shen

Zhang

Fang

et al. 2022

Gels

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Incorporating enzymes with three-dimensional (3D) printing is an exciting new field of convergence research that holds infinite potential for creating highly customizable components with diverse and efficient biocatalytic properties. Enzymes, nature’s nanoscale protein-based catalysts, perform crucial functions in biological systems and play increasingly important roles in modern chemical processing methods, cascade reactions, and sensor technologies. Immobilizing enzymes on solid carriers facilitates their recovery and reuse, improves stability and longevity, broadens applicability, and reduces overall processing and chemical conversion costs. Three-dimensional printing offers extraordinary flexibility for creating high-resolution complex structures that enable completely new reactor designs with versatile sub-micron functional features in macroscale objects. Immobilizing enzymes on or in 3D printed structures makes it possible to precisely control their spatial location for the optimal catalytic reaction. Combining the rapid advances in these two technologies is leading to completely new levels of control and precision in fabricating immobilized enzyme catalysts. The goal of this review is to promote further research by providing a critical discussion of 3D printed enzyme immobilization methods encompassing both post-printing immobilization and immobilization by physical entrapment during 3D printing. Especially, 3D printed gel matrix techniques offer mild single-step entrapment mechanisms that produce ideal environments for enzymes with high retention of catalytic function and unparalleled fabrication control. Examples from the literature, comparisons of the benefits and challenges of different combinations of the two technologies, novel approaches employed to enhance printed hydrogel physical properties, and an outlook on future directions are included to provide inspiration and insights for pursuing work in this promising field.

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Recent advances in polymer scaffolds for biomedical applications

Cited by 24 publications

References 338 publications

MCP-1-Loaded Poly(l-lactide-co-caprolactone) Fibrous Films Modulate Macrophage Polarization toward an Anti-inflammatory Phenotype and Improve Angiogenesis

MCP-1-Loaded Poly(l-lactide-co-caprolactone) Fibrous Films Modulate Macrophage Polarization toward an Anti-inflammatory Phenotype and Improve Angiogenesis

Enhancing Cell Growth with PAN/PVA-Gelatin 3D Scaffold: A Novel Approach using In-situ UV Radiation Electrospinning and Plasma Treatment

Advances in 3D Gel Printing for Enzyme Immobilization

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