Recent advances in polysaccharides from Lentinus edodes (Berk.): Isolation, structures and bioactivities

Sheng, Kangjia; Wang, Cuiling; Chen, Bitao; Kang, Min-Jae; Wang, Minchang; Liu, Ke; Wang, Ming

doi:10.1016/j.foodchem.2021.129883

Cited by 104 publications

(47 citation statements)

References 109 publications

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“…The above data suggested that acrolein-induced IEC-6 cells led to MMP loss, increased expression of caspase 3 and caspase 9 and decreased expression of the Bcl-2 protein, which indicated that PSG-1 effectively restored acrolein-induced apoptosis through the mitochondrial pathway. It had been established that the biological activity of mushroom polysaccharides were attributed to their chemical structure [ 14 , 37 ]. Thus, we speculated that the regulation of autophagy and apoptosis by PSG-1 was most likely related to the main structure β-(1 → 3, 1 → 6)-glucan, which was elucidated in our previous study [ 18 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

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Protective Effect of Ganoderma atrum Polysaccharide on Acrolein-Induced Apoptosis and Autophagic Flux in IEC-6 Cells

Wang¹,

Chang²,

Zheng³

et al. 2022

Foods

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This study was designed to explore the beneficial effect and mechanism of Ganoderma atrum (G. atrum) polysaccharide (PSG-1) on acrolein-induced IEC-6 cells. Our results indicated that PSG-1 significantly reduced the impairment of acrolein on cell viability, decreased oxidative stress, and enabled normal expression of tight junction (TJ) proteins that were inhibited by acrolein in IEC-6 cells. Furthermore, PSG-1 attenuated the elevation of microtubule-associated proteins light chain 3 (LC3) and Beclin 1-like protein 1 (Beclin 1) and increased the protein levels of phospho-mTOR (p-mTOR) and phospho-akt (p-akt), indicating that PSG-1 activated the mammalian target of rapamycin (mTOR) signaling pathway and alleviated acrolein-induced autophagy in IEC-6 cells. Moreover, PSG-1 markedly attenuated the acrolein-induced apoptosis, as evidenced by the increase in mitochondrial membrane potential (MMP) and B-cell lymphoma 2 (Bcl-2) expression, and the decrease in cysteine aspartate lyase (caspase)-3 and caspase-9. In addition, autophagy the inhibitor inhibited acrolein-induced TJ and apoptosis of IEC-6 cells, while the apoptosis inhibitor also inhibited acrolein-induced TJ and autophagy, suggesting that autophagy and apoptosis were mutually regulated. Taken together, the present study proved that PSG-1 could protect IEC-6 cells from acrolein-induced oxidative stress and could repair TJ by inhibiting apoptosis and autophagic flux, where autophagy and apoptosis were mutually regulated.

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Section: Discussionmentioning

confidence: 99%

“…Numerous studies have revealed that polysaccharides possessed great promises for food and health applications [ 14 , 15 , 16 ]. Ganoderma atrum ( G. atrum ) has been used as a medicine or diet with high nutritional value for thousands of years [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Ganoderma atrum Polysaccharide on Acrolein-Induced Apoptosis and Autophagic Flux in IEC-6 Cells

Wang¹,

Chang²,

Zheng³

et al. 2022

Foods

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“…Dynamic changes in yield and the molecular mass of polysaccharides consisting of fructose, galactose, glucose and mannose were found during the developmental stages of fruiting bodies of L. edodes [ 38 ]. A recent review summarised the work on these polysaccharides including the isolation methods, structural features, bioactivity mechanisms and structure-activity relationships [ 39 ]. Focussing on tumour-suppressing properties (among others lymphocyte proliferation and macrophage activation) in a mouse model, glycans isolated from the mycelia and fruiting body of P. ostreatus showed very similar properties as “immunoceuticals” [ 40 ].…”

Section: Polysaccharidesmentioning

confidence: 99%

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

et al. 2022

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Edible mushrooms are widely appreciated for their appealing flavours, low caloric values and high content of presumably health-protecting metabolites. Their long history of safe use together with the looming worldwide food crisis have revived the idea of generating meat analogues and protein isolates by the controlled fermentation of mycelia of these edible fungi as a dietary option. The occurrence of proteins, polysaccharides, smaller metabolites, metal ions and toxins in mycelia and fruiting bodies is compared among the three most popular species, Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), Lentinus edodes (shiitake) and some closely related species. Large effects of substrate chemistry, strain, developmental stage and ecological interactions result in a wide variation of the concentrations of some metabolites in both mycelial cells and fruiting bodies. This is obviously a result of the high adaptation abilities required to survive in natural habitats. Fungal bioprocesses are decoupled from agricultural production and can be operated anytime, anywhere, and on any scale according to demand. It is concluded that fungal biomass, if produced under food-grade conditions and on an industrial scale, could provide a safe and nutritious meat substitute and protein isolates with a high biological value for future vegan foods.

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“…For example, Inonotus obliquus polysaccharides are developed as functional and health products for reducing blood fat, removing free radicals, and regulating immune function [ 4 ]. Lentinus edodes polysaccharides are incorporated in various food/beverage products to obtain fortified functional foods/beverages for enhancing immune function, lowering serum lipid, lowering blood glucose, and regulating gut microbiota [ 5 ]. In addition, polysaccharides extracted from Ganoderma lucidum , Grifola frondosa , and Trametes versicolor have been developed as dietary supplements for promoting immune function [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques

Zhao

Guo

et al. 2021

Polymers

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In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared. Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%. D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (Mw), apparent viscosities, and molar ratios of constituent monosaccharides. In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest Mw and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest Mw and apparent viscosity. In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others. Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U. These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.

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Recent advances in polysaccharides from Lentinus edodes (Berk.): Isolation, structures and bioactivities

Cited by 104 publications

References 109 publications

Protective Effect of Ganoderma atrum Polysaccharide on Acrolein-Induced Apoptosis and Autophagic Flux in IEC-6 Cells

Protective Effect of Ganoderma atrum Polysaccharide on Acrolein-Induced Apoptosis and Autophagic Flux in IEC-6 Cells

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques

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