Recent advances in re-engineered liver: de-cellularization and re-cellularization techniques

Wang, Liren; Ying, Lin; Wang, Jiangtao; Pan, Liangliang; Huang, Kate; Wan, Li; Zhu, Gui‐Qi; Liu, Wen-Yue; Braddock, Martin; Zheng, Ming‐Hua

doi:10.1016/j.jcyt.2015.04.003

Cited by 7 publications

(10 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Eight studies evaluated and quantified the preservation of growth factors in a decellularized bioscaffold [12, 13, 15, 17, 18, 22, 24, 27]. Four studies did not perform the recellularization step [5, 12, 23, 24]. Regarding the cell types used in the recellularization step, seven studies used primary hepatocytes [8, 10, 13–15, 18, 27], three studies used liver progenitor cells [9, 11, 19], and six studies used immortalized hepatocytes and/or nonparenchymal cell lines [16, 17, 21, 22, 26, 27].…”

Section: Resultsmentioning

confidence: 99%

“…This technique consists of removing liver cells by perfusion with enzymes and/or detergent solutions, or by physical methods, to generate extracellular matrix- (ECM-) derived scaffolds while preserving vascular integrity. This is followed by the introduction of new cells with the appropriate characteristics and repopulation potential [5]. The efficiency and functionality of the bioengineered liver tissue can be tested by evaluating specific biomarkers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold

Rossi

Quintanilha

Nonaka

et al. 2019

Stem Cells International

View full text Add to dashboard Cite

The burden of liver diseases continues to grow worldwide, and liver transplantation is the only option for patients with end-stage liver disease. This procedure is limited by critical issues, including the low availability of donor organs; thus, novel therapeutic strategies are greatly needed. Recently, bioengineering approaches using decellularized liver scaffolds have been proposed as a novel strategy to overcome these challenges. The aim of this systematic literature review was to identify the major advances in the field of bioengineering using decellularized liver scaffolds and to identify obstacles and challenges for clinical application. The main findings of the articles and each contribution for technique optimization were highlighted, including the protocols of perfusion and decellularization, duration, demonstration of quality control—scaffold acellularity, matrix composition, and preservation of growth factors—and tissue functionality after recellularization. In previous years, many advances have been made as this technique has evolved from studies in animal models to human livers. As the field develops and this promising technique has become much more feasible, many challenges remain, including the selection of appropriate cell types for recellularization, route of cell administration, cell-seeding protocol, and scalability that must be standardized prior to clinical application.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold

Rossi

Quintanilha

Nonaka

et al. 2019

Stem Cells International

View full text Add to dashboard Cite

show abstract

“…It is composed of a layer of simple amniotic epithelium and matrix elements such as collagen (types I, III, IV, and V), HA, and various sulfated glycosaminoglycans that surround the UC vessels. The structural integrity of these components does not alter after the current decellularization process (Figure 1) [8,9].…”

Section: Characteristic and Preparation Of Wharton's Jellymentioning

confidence: 99%

Decellularized Wharton’s Jelly: Biomaterial Potential for Regenerative Medicine Applications - A Mini-Review

Gamba

Ribeiro

Simeoni

et al. 2023

Braz. arch. biol. technol.

View full text Add to dashboard Cite

The use of Wharton's Jelly (WJ) as a biomaterial is currently undergoing an appearance in the regenerative medicine field. The biomaterials applications focus on the aspects of cellular growth or delivery of proteins capable of stimulating cellular response. However, the basic knowledge about Wharton jelly and decellularization processing technology combined with understanding the physical-chemical properties of this biomaterial is necessary for proper application in regenerative medicine. This mini-review article summarizes information on the composition of WJ, application of drug delivery, in medicine and discusses recent HIGHLIGHTS • Wharton's Jelly (WJ) can stimulate cellular response.• WJ promotes cell proliferation, adhesion, differentiation, and migration.• WJ 3D printing scaffold can increase the healing tissue potential.• WJ contains a variety of growth factors that promote tissue regeneration.• developments with a special focus on its use for regenerative medicine. The most successful and stimulating applications are studies in regenerative medicine and tissue engineering, for wound healing to treat burns, tumor treatment, nanoparticle carriers, and drug delivery systems.

show abstract

“…A low concentration of cells may cause a poor seeding efficacy, while a high concentration of cells may result in cell death or vascular blockage. [32] Common recellularized seeding strategies can be accomplished by surface attachment and injection, whereas the seeding efficiency and uniformity of cells are difficult to guarantee. [33,34] To our knowledge, it is easier to reach the vasculature through perfusion culture to achieve uniform dispersion of cells into parenchyma and the formation of practical organs/tissues.…”

Section: Recellularizationmentioning

confidence: 99%

“…Moreover, the number of seeding cells is also very important. A low concentration of cells may cause a poor seeding efficacy, while a high concentration of cells may result in cell death or vascular blockage [32] …”

Section: Decellularization-recellularization Techniquementioning

confidence: 99%

Application of decellularization-recellularization technique in plastic and reconstructive surgery

Shang

Wang

Zhen

et al. 2023

Chinese Medical Journal

View full text Add to dashboard Cite

In the field of plastic and reconstructive surgery, the loss of organs or tissues caused by diseases or injuries has resulted in challenges, such as donor shortage and immunosuppression. In recent years, with the development of regenerative medicine, the decellularization-recellularization strategy seems to be a promising and attractive method to resolve these difficulties. The decellularized extracellular matrix contains no cells and genetic materials, while retaining the complex ultrastructure, and it can be used as a scaffold for cell seeding and subsequent transplantation, thereby promoting the regeneration of diseased or damaged tissues and organs. This review provided an overview of decellularization-recellularization technique, and mainly concentrated on the application of decellularization-recellularization technique in the field of plastic and reconstructive surgery, including the remodeling of skin, nose, ears, face, and limbs. Finally, we proposed the challenges in and the direction of future development of decellularization-recellularization technique in plastic surgery.

show abstract

Recent advances in re-engineered liver: de-cellularization and re-cellularization techniques

Cited by 7 publications

References 91 publications

Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold

Advances in Hepatic Tissue Bioengineering with Decellularized Liver Bioscaffold

Decellularized Wharton’s Jelly: Biomaterial Potential for Regenerative Medicine Applications - A Mini-Review

Application of decellularization-recellularization technique in plastic and reconstructive surgery

Contact Info

Product

Resources

About