Recent advances in research on microbial community in the composting process

Luo, Yun Mei; Shen, Jiayan; Wang, Xinxin; Xiao, Hongling; Yaser, Abu Zahrim; Fu, Jie

doi:10.1007/s13399-023-04616-9

Cited by 6 publications

(2 citation statements)

References 98 publications

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“…The microbial composition within composting systems is inherently dynamic, subject to influences from various factors, including the introduction of specific microbial strains [53,54]. In our study, the inoculation of Marasmius tricolor 310b brought about notable alterations in the fungal community structure.…”

Section: Marasmius Tricolor 310b and Fungal Community Dynamicsmentioning

confidence: 77%

The Impact of Marasmius tricolor 310b on the Degradation of Cellulose in Rapeseed Straw Composting

Wang,

Shi,

2023

Agronomy

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Composting, a vital process for organic waste management, hinges on intricate microbial dynamics. The role of specific microbial inoculants, such as fungi, in shaping these dynamics remains an area of keen interest. This study aimed to elucidate the impact of the fungal inoculant Marasmius tricolor 310b on composting dynamics, with a focus on temperature variations and the degradation of lignocellulosic components. We conducted a comprehensive composting experiment with four treatments: pure rapeseed straw, rapeseed straw with Marasmius tricolor 310b, a combination of rapeseed straw and pig manure, and the combination with Marasmius tricolor 310b. Temperature dynamics, lignocellulosic degradation rates, and fungal community structures were meticulously analyzed. While Marasmius tricolor 310b did not significantly alter the temperature profile, its presence markedly enhanced the degradation rates of cellulose and lignin. With the introduction of Marasmius tricolor 310b, the degradation rate of cellulose in the rapeseed straw compost increased from 0.0319 (ST) to 0.0419 (STM). In the combined rapeseed straw and pig manure compost, this rate rose from 0.0422 (STPM) to 0.045 (STPMM). For hemicellulose, the degradation rate in the rapeseed straw compost increased from 0.0103 (ST) to 0.014 (STM). However, in the combined compost, it slightly decreased from 0.0146 (STPM) to 0.013 (STPMM). As for lignin, its degradation rate in the rapeseed straw compost saw an increase from 0.0024 (ST) to 0.00366 (STM). In the combined compost, the rate rose from 0.00302 (STPM) to 0.00402 (STPMM). Fungal community analyses revealed discernible shifts in structure and diversity upon the fungus’s introduction. Network analyses further highlighted a highly interconnected microbial community, suggesting its robustness against perturbations. Temporal dynamics underscored the intricate interplay of microbial interactions, substrate availability, and external inoculants. The introduction of Marasmius tricolor 310b profoundly influences composting dynamics, offering valuable insights into microbial community interactions and presenting potential avenues for optimizing composting strategies.

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Section: Marasmius Tricolor 310b and Fungal Community Dynamicsmentioning

confidence: 77%

The Impact of Marasmius tricolor 310b on the Degradation of Cellulose in Rapeseed Straw Composting

Wang,

Shi,

2023

Agronomy

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“…The emission of GHGs such as methane during anaerobic microbial degradation is a complex process, and it is poorly understood in terms of determining the GHG emissions from livestock waste. To understand the role of microbes in GHG emissions, the separation, identification, and analysis of DNA fragments are crucial, and there are multiple recent advances to improve the existing understanding of the structure and function of microbes and biological methane emissions [6][7][8]. Considering livestock waste is a significant source of GHG emission, additional research is needed to develop pragmatic approaches for on-farm manure management.…”

Section: Introductionmentioning

confidence: 99%

Denaturing Gradient Gel Electrophoresis Approach for Microbial Shift Analysis in Thermophilic and Mesophilic Anaerobic Digestions

Pandey,

Chowdhury,

Wang

2024

Gels

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To determine the evolution of microbial community and microbial shift under anaerobic processes, this study investigates the use of denaturing gradient gel electrophoresis (DGGE). In the DGGE, short- and medium-sized DNA fragments are separated based on their melting characteristics, and this technique is used in this study to understand the dominant bacterial community in mesophilic and thermophilic anaerobic digestion processes. Dairy manure is known for emitting greenhouse gases (GHGs) such as methane, and GHG emissions from manure is a biological process that is largely dependent on the manure conditions, microbial community presence in manure, and their functions. Additional efforts are needed to understand the GHG emissions from manure and develop control strategies to minimize the biological GHG emissions from manure. To study the microbial shift during anaerobic processes responsible for GHG emission, we conducted a series of manure anaerobic digestion experiments, and these experiments were conducted in lab-scale reactors operated under various temperature conditions (28 °C, 36 °C, 44 °C, and 52 °C). We examined the third variable region (V3) of the 16S rRNA gene fingerprints of bacterial presence in anaerobic environment by PCR amplification and DGGE separation. Results showed that bacterial community was affected by the temperature conditions and anaerobic incubation time of manure. The microbial community structure of the original manure changed over time during anaerobic processes, and the community composition changed substantially with the temperature of the anaerobic process. At Day 0, the sequence similarity confirmed that most of the bacteria were similar (>95%) to Acinetobacter sp. (strain: ATCC 31012), a Gram-negative bacteria, regardless of temperature conditions. At day 7, the sequence similarity of DNA fragments of reactors (28 °C) was similar to Acinetobacter sp.; however, the DNA fragments of effluent of reactors at 44 °C and 52 °C were similar to Coprothermobacter proteolyticus (strain: DSM 5265) (similarity: 97%) and Tepidimicrobium ferriphilum (strain: DSM 16624) (similarity: 100%), respectively. At day 60, the analysis showed that DNA fragments of effluent of 28 °C reactor were similar to Galbibacter mesophilus (strain: NBRC 10162) (similarity: 87%), and DNA fragments of effluent of 36 °C reactors were similar to Syntrophomonas curvata (strain: GB8-1) (similarity: 91%). In reactors with a relatively higher temperature, the DNA fragments of effluent of 44 °C reactor were similar to Dielma fastidiosa (strain: JC13) (similarity: 86%), and the DNA fragments of effluent of 52 °C reactor were similar to Coprothermobacter proteolyticus (strain: DSM 5265) (similarity: 99%). To authors’ knowledge, this is one of the few studies where DGGE-based approach is utilized to study and compare microbial shifts under mesophilic and thermophilic anaerobic digestions of manure simultaneously. While there were challenges in identifying the bands during gradient gel electrophoresis, the joint use of DGGE and sequencing tool can be potentially useful for illustrating and comparing the change in microbial community structure under complex anaerobic processes and functionality of microbes for understanding the consequential GHG emissions from manure.

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