Recent Advances in Solid-Phase Microextraction for Forensic Applications

Prada, Paola A.; Furton, Kenneth G.

doi:10.1016/b978-0-12-381373-2.00117-4

Cited by 11 publications

(4 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SPME sampling has unique characteristics, such as the yield of clean concentrated extracts from heterogeneous matrices [4,5] and negligible depletion of the system that allows for repeated sampling without disturbance of that system [6,7]. At present, SPME has been validated for many different bioanalytical and forensic studies, including ex vivo and in vivo sampling for a variety of pharmaceuticals [5,[8][9][10][11][12][13][14][15][16][17][18]. Of particular interest are minimally invasive studies into the biokinetics of pharmaceuticals and drugs.…”

Section: Introductionmentioning

confidence: 98%

Sorption of amitriptyline and amphetamine to mixed-mode solid-phase microextraction in different test conditions

Peltenburg

Droge

Hermens

et al. 2015

Journal of Chromatography A

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 98%

Sorption of amitriptyline and amphetamine to mixed-mode solid-phase microextraction in different test conditions

Peltenburg

Droge

Hermens

et al. 2015

Journal of Chromatography A

View full text Add to dashboard Cite

“…Thus, it is very important to have proper dog training procedures. In Europe, the general method can be divided into two protocols: the tube-retrieving system and the cloth responding system (Prada and Furton 2012).…”

Section: Resultsmentioning

confidence: 99%

Volatile organic compounds: instrumental and canine detections link an individual to the crime scene

Filetti

Mizio

Rendine

et al. 2019

Egypt J Forensic Sci

View full text Add to dashboard Cite

Background: Whenever a crime is committed, forensic personnel are requested to collect every kind of evidence to establish the relationship between the suspects and the crime. When any evidence is accidentally destroyed or not found, there is one type of latent evidence that is always deposited at the crime scene: unique human scent. Recently, the use of trained canines to detect selective human scent at a crime scene has increased. To consolidate this kind of evidence, it is essential to have an exact knowledge and an awareness of the chemical signature of the volatile compounds that could indicate the presence of the alleged offender at the crime scene. This experimental study aims to detect the volatile organic compounds (VOCs) released from subjects who handled scent-articles to imprint their odor on. After handling, each scent-article was wrapped in sterile and VOC-free cotton gauzes for 48 h for secondary transfer. VOCs were detected by headspace/solid-phase microextraction-gas chromatography/mass spectrometry (HS/SPME-GC/MS) and well-trained dogs, at different time points (up to 15 days). Furthermore, the possibility of further DNA detection after contact was also investigated to propose a novel approach able to identify a subject from this latent forensic trace. Results: Data show that inter-individual human scent composition includes different VOCs, but dogs were able to discriminate the individual who touched the object at the crime scene. The dog training procedure showed excellent sensitivity (between 99.48 and 100%) and specificity (between 60 and 100%), having a positive predictive value (PPV) ranging between 97.94 and 100% and a negative predictive value (NPV) ranging between 85.71 and 100%. Preliminary work on DNA analysis released after contact yielded positive results, even if further studies are necessary, expanding the same experimentation to a larger sample with the aim of obtaining a statistically significant result. Conclusion: Data show that human scent is a good source of VOCs and a good target for canine training. The welltrained dog represents a specialized biological device able to discriminate personal human odor from any contaminants in the mixture detected by instrumental analysis. Furthermore, this study proposes the use of human scent as a forensic latent trace for DNA profiling.

show abstract

“…The underlying biological pathways that produce these compounds in sweat, sebum, and epidermis are quite varied and the compounds of interest have a wide range of polarity, size, and structure as a result. These diverging compounds are of interest to clinical research fields such as forensics and toxicology [11][12][13], not to mention the obvious applications in medicinal diagnosis [14][15][16], doping tests [17,18], and environmental/occupational exposomics [19][20][21].…”

Section: Skin Samplingmentioning

confidence: 99%

Advancements in Non-Invasive Biological Surface Sampling and Emerging Applications

Nalbant

Boyacı

2019

Separations

View full text Add to dashboard Cite

Biological surfaces such as skin and ocular surface provide a plethora of information about the underlying biological activity of living organisms. However, they pose unique problems arising from their innate complexity, constant exposure of the surface to the surrounding elements, and the general requirement of any sampling method to be as minimally invasive as possible. Therefore, it is challenging but also rewarding to develop novel analytical tools that are suitable for in vivo and in situ sampling from biological surfaces. In this context, wearable extraction devices including passive samplers, extractive patches, and different microextraction technologies come forward as versatile, low-invasive, fast, and reliable sampling and sample preparation tools that are applicable for in vivo and in situ sampling. This review aims to address recent developments in non-invasive in vivo and in situ sampling methods from biological surfaces that introduce new ways and improve upon existing ones. Directions for the development of future technology and potential areas of applications such as clinical, bioanalytical, and doping analyses will also be discussed. These advancements include various types of passive samplers, hydrogels, and polydimethylsiloxane (PDMS) patches/microarrays, and other wearable extraction devices used mainly in skin sampling, among other novel techniques developed for ocular surface and oral tissue/fluid sampling.

show abstract

Recent Advances in Solid-Phase Microextraction for Forensic Applications

Cited by 11 publications

References 98 publications

Sorption of amitriptyline and amphetamine to mixed-mode solid-phase microextraction in different test conditions

Sorption of amitriptyline and amphetamine to mixed-mode solid-phase microextraction in different test conditions

Volatile organic compounds: instrumental and canine detections link an individual to the crime scene

Advancements in Non-Invasive Biological Surface Sampling and Emerging Applications

Contact Info

Product

Resources

About