Recent advances in the analysis of biological particles by capillary electrophoresis

Abstract

This review covers research papers published in the years 2005-2007 that describe the application of capillary electrophoresis to the analysis of biological particles such as whole cells, subcellular organelles, viruses and microorganisms.

Recent developments in capillary and chip electrophoresis of bioparticles: Viruses, organelles, and cells

“…The present paper is conceived as a follow-up of the reviews by Kostal and Arriaga [5] and Kremser et al [6], both published in this journal. It includes mainly research papers that appeared between 2008 and 2010, and some articles from 2007 not included in former reviews; it deals with the analysis of big biological assemblies, including cellorganelles, viruses, and entire bacteria and yeast cells by means of isoelectric focusing (CIEF) and zone electrophoresis (CZE), both in the capillary or in the chip format.…”

“…mitochondria) by means of a post-column LIF detector that uses a sheath flow cuvette [19]. Several articles have been published on this topic in the recent years [5], mainly employing poly(acryloylaminopropanol) (AAP)-coated capillaries and HEPES buffers containing sucrose as BGE (see Table 1). Poly(acryloylaminopropanol) is used to reduce the electroosmotic flow and to minimize the interactions between the proteins of the mitochondrial membrane and the capillary wall, and sucrose stabilizes mitochondria against osmotic rupture of the membrane.…”

Capillary and Microchip Electrophoresis

“…Bioparticles exhibit a surface charge due to charged or chargeable functional groups at their surfaces and can thus migrate under the influence of an electric field and become separated by CZE or CIEF. CE is thereby employed to characterize and detect the bioparticles [175][176][177]. The emergence of nanotechnology and nanobiotechnology has required the development of methodologies to determine and characterize nanoparticles (!…”

Electrophoretic mobility of a spherical liposome

“…Therefore, characterization of the surface charge properties under various conditions is of fundamental importance for liposome applications such as complexation with oppositely charged polyelectrolyte. Capillary electrophoresis has emerged as a suitable tool for analyzing the surface charge via measuring the electrophoretic velocity of a liposome subject to an imposed electric field [5][6][7][8][9][10].…”