Recent advances in the bioprospection and applications of chitinolytic bacteria for valorization of waste chitin

Dhole, Neeraja P.; Dar, Mudasir A.; Pandit, Radhakrishna S.

doi:10.1007/s00203-021-02234-5

Cited by 23 publications

(5 citation statements)

References 106 publications

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“…Besides antibiotics, actinomycetes are prolific producers of chitinases. Both mesophilic genera, such as Streptomyces , Saccharothrix , and Microbispora , and thermophilic genera, such as Thermobifida and Streptosporangium , are reported to produce chitinases [ 8 , 10 ]. A chitinase-producing actinomycete, Saccharothrix yanglingensis Hhs.015, was isolated from the roots of cucumber, which produced eight different chitinases [ 55 ].…”

Section: Sources Of Chitinasesmentioning

confidence: 99%

“…Further, since chitinous waste is generated in abundance, its sustainable valorization is essential to prevent environmental pollution. In this context, chitin-active enzymes are critical toolboxes for chitin waste management, with simultaneous generation of value-added products [ 8 ]. Apart from their myriad of industrial applications, chitinases, as biocontrol agents, are gaining popularity in the agricultural sector, for increasing crop productivity.…”

Section: Introductionmentioning

confidence: 99%

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Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Poria

Rana

Kumari

et al. 2021

Biology

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Chitinases are a large and diversified category of enzymes that break down chitin, the world’s second most prevalent polymer after cellulose. GH18 is the most studied family of chitinases, even though chitinolytic enzymes come from a variety of glycosyl hydrolase (GH) families. Most of the distinct GH families, as well as the unique structural and catalytic features of various chitinolytic enzymes, have been thoroughly explored to demonstrate their use in the development of tailor-made chitinases by protein engineering. Although chitin-degrading enzymes may be found in plants and other organisms, such as arthropods, mollusks, protozoans, and nematodes, microbial chitinases are a promising and sustainable option for industrial production. Despite this, the inducible nature, low titer, high production expenses, and susceptibility to severe environments are barriers to upscaling microbial chitinase production. The goal of this study is to address all of the elements that influence microbial fermentation for chitinase production, as well as the purifying procedures for attaining high-quality yield and purity.

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Section: Sources Of Chitinasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Poria

Rana

Kumari

et al. 2021

Biology

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“…The CS: CZ ratio varied from 1.63 to 4.08. The significance of bacterial endophytes delivering chitinase in a colloidal chitin-containing environment with similar CS: CZ has been previously investigated 41 , 42 , wherein the isolated microflora from multiple agronomic crop studies had the CS: CZ ratio ranging from 1–6. After optimizing various carbon source supplementations under 5 incubation periods, the average chitinase activity was significantly highest in glycerol supplementation and least in maltose.…”

Section: Discussionmentioning

confidence: 99%

Dynamics, phylogeny and phyto-stimulating potential of chitinase synthesizing bacterial root endosymbiosiome of North Western Himalayan Brassica rapa L.

Padder

Rather

Bhat

et al. 2022

Sci Rep

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The less phytopathogen susceptibility in Himalayan Brassica rapa L. has made it an exceptional crop eluding synthetic pesticide inputs, thereby guarantying economically well-founded and ecologically sustainable agriculture. The relevance of niche microflora of this crop has not been deliberated in this context, as endosymbiosiome is more stable than their rhizosphere counterparts on account of their restricted acquaintance with altering environment; therefore, the present investigation was carried out to study the endophytic microfloral dynamics across the B. rapa germplasm in context to their ability to produce chitinase and to characterize the screened microflora for functional and biochemical comportments in relevance to plant growth stimulation. A total of 200 colonies of bacterial endophytes were isolated from the roots of B. rapa across the J&K UT, comprising 66 locations. After morphological, ARDRA, and sequence analysis, eighty-one isolates were selected for the study, among the isolated microflora Pseudomonas sp. Bacillus sp. dominated. Likewise, class γ-proteobacteria dominated, followed by Firmicutes. The diversity studies have exposed changing fallouts on all the critical diversity indices, and while screening the isolated microflora for chitinase production, twenty-two strains pertaining to different genera produced chitinase. After carbon source supplementation to the chitinase production media, the average chitinase activity was significantly highest in glycerol supplementation. These 22 strains were further studied, and upon screening them for their fungistatic behavior against six fungal species, wide diversity was observed in this context. The antibiotic sensitivity pattern of the isolated strains against chloramphenicol, rifampicin, amikacin, erythromycin, and polymyxin-B showed that the strains were primarily sensitive to chloramphenicol and erythromycin. Among all the strains, only eleven produced indole acetic acid, ten were able to solubilize tricalcium phosphate and eight produced siderophores. The hydrocyanic acid and ammonia production was observed in seven strains each. Thus, the present investigation revealed that these strains could be used as potential plant growth promoters in sustainable agriculture systems besides putative biocontrol agents.

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“…They play a crucial role in the degradation of chitin in various biogeochemical ecosystems (Beier and Bertilsson 2013 ). Bacteria in marine habitats and genera like Bacillus produce chitinases involved in the degradation of recalcitrant materials in the ocean (Dhole et al 2021 ). Purification and characterization studies infer that they possess a broad range of pH, temperature and isoelectric points, depending on their isolation points (Rathore and Gupta 2015 ).…”

Section: Chitinases: Sources Function and Microbial Productionmentioning

confidence: 99%

Chitinases: expanding the boundaries of knowledge beyond routinized chitin degradation.

Unuofin,

Odeniyi,

Majengbasan

et al. 2024

Environ Sci Pollut Res

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Chitinases, enzymes that degrade chitin, have long been studied for their role in various biological processes. They play crucial roles in the moulting process of invertebrates, the digestion of chitinous food, and defense against chitin-bearing pathogens. Additionally, chitinases are involved in physiological functions in crustaceans, such as chitinous food digestion, moulting, and stress response. Moreover, chitinases are universally distributed in organisms from viruses to mammals and have diverse functions including tissue degradation and remodeling, nutrition uptake, pathogen invasion, and immune response regulation. The discovery of these diverse functions expands our understanding of the biological significance and potential applications of chitinases. However, recent research has shown that chitinases possess several other functions beyond just chitin degradation. Their potential as biopesticides, therapeutic agents, and tools for bioremediation underscores their significance in addressing global challenges. More importantly, we noted that they may be applied as bioweapons if ethical regulations regarding production, engineering and application are overlooked.

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Recent advances in the bioprospection and applications of chitinolytic bacteria for valorization of waste chitin

Cited by 23 publications

References 106 publications

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications

Dynamics, phylogeny and phyto-stimulating potential of chitinase synthesizing bacterial root endosymbiosiome of North Western Himalayan Brassica rapa L.

Chitinases: expanding the boundaries of knowledge beyond routinized chitin degradation.

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