Recent advances in the chemistry and biochemistry of inositol phosphates of biological interest

Interaction of synthetic D‐6‐deoxy‐myo‐inositol 1,4,5‐trisphosphate with the Ca2+‐releasing D‐myo‐inositol 1,4,5‐trisphosphate receptor, and the metabolic enzymes 5‐phosphatase and 3‐kinase

“…Ins(l,4,5)Pa 3-ki,mse and 5-phosphatase, and the chemical design of novel lns(l,4,5)P3 agomsts, antagonists and enzyme mh~bltors. Recent progress m inositol phosphate chemistry [7,8] and the molecular recogmtion of Ins(l,4,5)P3 by these three proteins has been revzewed [9].…”

“…As yet, however, little structure-activity data have emerged, apart from recognition of the fundamental importance of the vicinaI D-4,S-btsphosphat¢ moiety [8,9]. We report here a study on the interaction of the synthetic analogue D-6.deoxy-myo mosstol 1,4,5-tnsphosphate (6-deoxy-lns(l,4,5)P3, (2), thloat¢ (D-Ins(l,4,5)PS~) was syntheszzed and purified s=mdarly to the procedu-e described for racemtc material [I01 D-Ins(l,4,S)Pa was from Calbiochem All cell culture reagents were from G1bco 4SCaCl2 (approx I000 C~/mmol) was from Amersham International ECso ~a[ues were derived using ALLF[T computer-asslsted curve fltung [18] Combined data from a number of Independent experiments (n) are expressed as mean ± SEM, where n >_.…”

Mining of Ruminant Microbial Phytase (RPHY1) from Metagenomic Data of Mehsani Buffalo Breed: Identification, Gene Cloning, and Characterization

“…They are high-molecular-weight acid phosphatases that sequentially cleave the phosphate ester bonds of phytate (IP6) (myo-inositol-hexakisphos-phate) [Angel et al, 2002;Potter, 1990;Wyss et al, 1999]. Phytate or phytic acid is the main chemical form of phosphorus in plant tissues, constituting >60% of the total phosphorus plant content [Cosgrove, 1966;Reddy et al, 1982].…”

Practical divergent synthesis of all possible regioisomers of myo-inositol trisphosphates

“…I ( 1,4,6)P3,9 I( 1 ,5,6)P3,lo I(2,4,5)P31 1 and I(2,4,6)P3.7 Here we report a simple, divergent synthesis of all the possible 12 regioisomers of IP3s via inositol tribenzoates (IBz3).…”

“…D-myo-Inositol-1,4,5-trisphosphate [I( 1,4,5)P3] receptors are a homologous family of tetrameric ligand-gated Ca2+ channels which allow mobilization of intracellular Ca2+ stores in response to activation of cell-surface receptors linked to I ( 1,4,5)P3 generation. Although the ligand recognition site of the IP3 receptors appears to be in the N-terminal region, the exact nature of the molecular interactions is not understood.…”