Recent advances in the combination of capillary electrophoresis with mass spectrometry: From element to single‐cell analysis

Abstract

This overview deals with the latest development of electrophoresis in capillaries and microfluidic devices coupled to MS detection. A wide selection of relevant articles covers the literature published from January 2010 till June 2012 as a continuation of the review article on the same topic by Pantuckova et al. [Electrophoresis 2011, 32, 43-51]. Special attention is paid to the new improvements in instrumentation and methodology of three interfacing methods, ESI, matrix-assisted desorption/ionization, and ICP. Representative examples illustrate applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis and single-cell analysis. The combinations of MS with capillary versions of electrochromatography, ITP, IEF, and micellar electrokinetic chromatography are not included.

Categorizing Cells on the Basis of their Chemical Profiles: Progress in Single-Cell Mass Spectrometry

“…It features rapid analyte separations based on the electrophoretic mobility of molecules, including those with the same molecular weights (e.g., diastereomers), with high resolving power and low sample consumption (a microliter or less). 31,32 Many aspects of CE have greatly progressed in recent decades, and include the development of advanced separation modes and nanoscale sampling, and the interface of CE with different detection methods. 30,67 While CE is powerful on its own, it is even more productive when coupled with optical, electrochemical, or MS-based detection.…”

Recent advances in capillary electrophoresis instrumentation for the analysis of explosives

“…Two of them, which contained 2,4-dinitroanisole (DNAN), 1,2,4-triazon-5-one (NTO), 1-nitroguanidine (NQ) and RDX were subjected to analysis, reaching detection limits ranging from 0.007 to 0.009 mg L −1 [48] Finally, note that CE coupled with MS techniques provides additional selectivity compared to CE with DAD detection and allows the confirmation of the detected molecules. This hyphenated technique has been used in the forensic analysis of some samples such as drugs (amphetamine, methadone and morphine) [49], and also in metabolomics. [50] Nevertheless, it has limited application in the analysis of explosives and their residues [49] and no studies have been published during the reviewed period.…”

“…This hyphenated technique has been used in the forensic analysis of some samples such as drugs (amphetamine, methadone and morphine) [49], and also in metabolomics. [50] Nevertheless, it has limited application in the analysis of explosives and their residues [49] and no studies have been published during the reviewed period.…”

Developments in Interfacing Designs for CE–MS: Towards Enabling Tools for Proteomics and Metabolomics

“…Recently, the field of CE-MS has benefitted from improvements in interfacing designs and, as a result, CE-MS gained significant interest as a tool for proteomics, glycomics, metabolomics and bio-analytical research [2,[17][18][19]. These fields can profit from enhanced sensitivity, as biological samples often contain many low-abundant species and may also be volume-restricted.…”