Recent advances in the design of injectable hydrogels for stem cell-based therapy

Liang, Kun; Bae, Ki Hyun; Kurisawa, Motoichi

doi:10.1039/c9tb00485h

Cited by 82 publications

(69 citation statements)

References 124 publications

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“…[1][2][3] Nowadays, injectable hydrogels have gained signicant traction in tissue engineering, as they exhibit greater accessibility to sites of injury and minimal invasiveness with patient discomfort signicantly reduced aer delivery compared with the pre-fabricated scaffold. [4][5][6][7][8] As an integral component of the ECM, hyaluronic acid (HA) is involved in many cellular processes including motility, signaling and matrix remodeling. 4,9 Along with its favourable properties such as inherent biocompatibility, water adsorption ability and biodegradability, mediated by natural hyaluronidases in the body, HA-based hydrogel has been considered as one of the most suitable biomaterials for a variety of tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6][7][8] As an integral component of the ECM, hyaluronic acid (HA) is involved in many cellular processes including motility, signaling and matrix remodeling. 4,9 Along with its favourable properties such as inherent biocompatibility, water adsorption ability and biodegradability, mediated by natural hyaluronidases in the body, HA-based hydrogel has been considered as one of the most suitable biomaterials for a variety of tissue engineering applications. [10][11][12][13][14] Despite these outstanding features, its further applicability is still hindered by some shortcomings, such as, low mechanical strength and quick degradation kinetics.…”

Section: Introductionmentioning

confidence: 99%

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Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering

Wang

Zhang

et al. 2020

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering

Wang

Zhang

et al. 2020

RSC Adv.

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“…Injectable hydrogels derived from natural polymers, such as silk fibroin, alginate, hyaluronic acid and collagen, have been regularly used and show great potential for biomedical applications, including the delivery of local therapeutic agents [ 8 ]. The promising advantages of these biomaterials come from their adaptability and versatility due to their properties and components being similar to those of the physiological extracellular matrix [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

et al. 2020

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Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty–GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Ty–GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2–5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Ty–GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Ty–GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.

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“…But their low bio‐stability and possible immunogenicity in vivo would prevent further clinical application. Currently, there has been an increasing emphasis on elucidating the influence of physical properties of the microenvironment on stem cell fate (Chaudhuri et al, 2016; Liang, Bae, & Kurisawa, 2019; Ma et al, 2018). Considered as the “fourth element” of tissue engineering, external physical stimulatory cues have the advantages of high stability and non‐immunogenicity and thus show great application prospect in guiding stem cell fate and stem‐cell‐based tissue engineering (Du, Guo, Wang, Mikos, & Zhang, 2019).…”

Section: Introductionmentioning

confidence: 99%

Control the fate of human umbilical cord mesenchymal stem cells with dual‐enzymatically cross‐linked gelatin hydrogels for potential applications in nerve regeneration

Gao

et al. 2020

J Tissue Eng Regen Med

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Stem‐cell‐based therapy is a promising strategy to treat challenging neurological diseases, while its application is hindered primarily by the low viability and uncontrolled differentiation of stem cell. Hydrogel can be properly engineered to share similar characteristics with the target tissue, thus promoting cell viability and directing cell differentiation. In this study, we proposed a new dual‐enzymatically cross‐linked and injectable gelatin hydrogel for regulating survival, proliferation, and differentiation of human umbilical cord mesenchymal stem cells (hUC‐MSCs) in a three‐dimensional matrix. This injectable gelatin hydrogel was formed by oxidative coupling of gelatin–hydroxyphenyl acid conjugates catalyzed by hydrogen horseradish peroxidase (HRP) and choline oxidase (ChOx). Modulus and H2O2 release can be well controlled by ChOx activity. Results from calcein‐AM/PI staining and Ki67 immunofluorescence tests demonstrated that the survival and proliferation behavior of hUC‐MSCs were highly enhanced in HRP1UChOx0.25U hydrogel with lower modulus and less H2O2 release compared with other groups. Attractively, the expression of neuron‐specific markers β‐III tubulin, neurofilament light chain (NFL), and synapsin‐1 was significantly increased in HRP1UChOx0.25U hydrogel as well. Additionally, in vitro hemolysis test and in vivo HE staining data highlighted the good biocompatibility. Undoubtedly, this injectable gelatin hydrogel's ability to control hUC‐MSCs' fate holds enormous potentials in nervous disorders' therapy and nerve regeneration.

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Recent advances in the design of injectable hydrogels for stem cell-based therapy

Abstract: The recent advances in the design of injectable hydrogels for stem cell delivery, especially for in vivo applications, are overviewed in this review.

Cited by 82 publications

References 124 publications

Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering

Dual-enzymatically crosslinked and injectable hyaluronic acid hydrogels for potential application in tissue engineering

Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

Control the fate of human umbilical cord mesenchymal stem cells with dual‐enzymatically cross‐linked gelatin hydrogels for potential applications in nerve regeneration

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