Developing an accurate, reliable, and stable glucose detection sensor poses a formidable challenge for the food industry. In this study, hydrothermal and wet chemical methods were employed to fabricate a Pt single-atom catalyst on Co 3 O 4 (Co 3 O 4 /Pt SAs), where dispersed Pt atoms collaboratively catalyze the electrochemical reaction on the electrode surface. Subsequently, the integration of the microelectrode with polyethylene glycol diacrylate (PEG-DA) hydrogel and a dualchannel microfluidic chip facilitated continuous and rapid glucose detection. The addition of 5% PEG-DA hydrogel served to eliminate interference and expedite glucose detection, while the design of the dual-channel microfluidic chip generated microscale vortices, providing ample reaction opportunities for the target glucose while preventing interference from the mixing of previous and current reaction solutions. The research exhibited a favorable linear detection range from 1 to 800 μM, with a detection limit of 0.84 μM. Furthermore, validation of the device's feasibility in real samples demonstrated a glucose recovery rate ranging from 94.9 to 104.4% in beverages.