Recent advances in the hyphenation of electromigration techniques with mass spectrometry

Posta, Susanna Della; Fanali, Chiara; Gallo, Valéria; Fanali, Salvatore

doi:10.1016/j.trac.2022.116800

Cited by 8 publications

(5 citation statements)

References 75 publications

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“…Advances in CEC for the analysis of proteins were recently reviewed by Hajba and Guttman [198] and general CEC‐MS hyphenation in the paper by Della Posta et al. [36].…”

Section: Ce‐mass Spectrometrymentioning

confidence: 99%

“…The open-tubular mode of CEC with a stationary phase immobilized on the inner fused silica capillary wall has several advantages, such as easy preparation, avoidance of back-pressure problems, bubble formation, and frit fabrication [195]. A poly(norepinephrine)-coated open-tubular column for the separation of various basic and acidic proteins and glycoforms of recombinant human erythropoietin [196] and a fibrin coating for mAb variants characterization [197] were developed by Xiao et al Advances in CEC for the analysis of proteins were recently reviewed by Hajba and Guttman [198] and general CEC-MS hyphenation in the paper by Della Posta et al [36].…”

Section: 35mentioning

confidence: 99%

“…Although the development was initially put into LC-MS systems due to its straightforward coupling and better stability of operation, the hyphenation of CE with highly selective and sensitive MS is emerging as a promising bioanalytical tool [32][33][34][35][36]. The hyphenation of MS detector to CE has advantages such as a lower detection limit than UV, no need for chromophore and fluorophore groups in the analyte, subsequent resolution of comigrating analytes, and elucidation of the structure of unknown molecules.…”

Section: Introductionmentioning

confidence: 99%

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Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Maráková

Opetová

Tomašovský

2023

J of Separation Science

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Capillary electrophoresis is recognized as a valued separation technique for its high separation efficiency, low sample consumption, good economic and ecological aspects, reproducibility, and complementarity to traditional liquid chromatography techniques. Capillary electrophoresis experiments are generally performed utilizing optical detection, such as ultraviolet or fluorescence detectors. However, in order to provide structural information, capillary electrophoresis hyphenated to highly sensitive and selective mass spectrometry has been developed to overcome the limitations of optical detections. Capillary electrophoresis‐mass spectrometry is increasingly popular in protein analysis, including biopharmaceutical and biomedical research. It is frequently applied for the determination of physicochemical and biochemical parameters of proteins, offers excellent performance for in‐depth characterizations of biopharmaceuticals at various levels of analysis, and has been also already proven as a promising tool in biomarker discovery. In this review, we focus on the possibilities and limitations of capillary electrophoresis‐mass spectrometry for protein analysis at their intact level. Various capillary electrophoresis modes and capillary electrophoresis‐mass spectrometry interfaces, as well as approaches to prevent protein adsorption and to enhance sample loading capacity, are discussed and the recent (2018–March 2023) developments and applications in the field of biopharmaceutical and biomedical analysis are summarized.

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“…Advances in CEC for the analysis of proteins were recently reviewed by Hajba and Guttman [198] and general CEC‐MS hyphenation in the paper by Della Posta et al. [36].…”

Section: Ce‐mass Spectrometrymentioning

confidence: 99%

Section: 35mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Maráková

Opetová

Tomašovský

2023

J of Separation Science

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“…Being both universal and selective and providing high‐sensitivity and structurally rich information on the analyzed compounds, MS represents the most powerful detection mode for CE, LC, and other separation methods. The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE–MS in general [195–198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8, 16, 44, 199–201]. Importance of MS as analytical and structure elucidation method has significantly increased in the last years due to its crucial role in the previous omics technologies [44, 199, 202].…”

Section: Detectionmentioning

confidence: 99%

“…The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE-MS in general [195][196][197][198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8,16,44,[199][200][201].…”

Section: Mass Spectrometrymentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Capillary Electrophoresis in Food Analysis

Castro‐Puyana,

Bernardo‐Bermejo,

Abad‐Gil

et al. 2024

Encyclopedia of Analytical Chemistry

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The development and application of faster and more powerful analytical methodologies in Food Science is essential to face the demands from regulatory agencies, official laboratories, and consumers. In this field, capillary electrophoresis (CE) is nowadays a well‐established separation technique for food analysis that possesses exceptional properties related to high analysis speed and separation efficiencies combined with the small samples and reagent requirements. Moreover, it is a very versatile technique since different CE modes can be employed for a broad range of applications and separation challenges. In fact, CE should be considered as the first option when dealing with highly polar, charged, or chiral analytes. In the past two decades, a high amount of research work has been published, demonstrating the high potential of CE to analyze different food components in a broad variety of food matrices. The aim of this article is to provide an overview of the use of CE for food analysis including a summary of the characteristics of the CE modes and the main detectors mostly employed in this field. Besides, a wide view of the latest applications of CE in food analysis (2021–2023) is also provided, demonstrating the exceptional possibilities of this analytical technique.

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Recent advances in the hyphenation of electromigration techniques with mass spectrometry

Cited by 8 publications

References 75 publications

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Capillary Electrophoresis in Food Analysis

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