2020
DOI: 10.1016/j.isci.2020.101801
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Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging

Abstract: Summary Nucleic acids, aside from being best known as the carrier of genetic information, are versatile biomaterials for constructing nanoscopic devices for biointerfacing, owing to their unique properties such as specific base pairing and predictable structure. For live-cell analysis of native RNA transcripts, the most widely used nucleic acid-based nanodevice has been the molecular beacon (MB), a class of stem-loop-forming probes that is activated to fluoresce upon hybridization with target RNA. H… Show more

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Cited by 36 publications
(33 citation statements)
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“…On the other hand, the dual-terminal labeling strategy should be useful for large-scale and facile production of any RNA beacon in principle. RNA beacons can be used for miRNA detection, visualization of microRNA maturation, specific RNA imaging, separation of cell populations, and identification of inhibitors of RNA-binding protein [43][44][45][46][47][48] . Therefore, it is anticipated that the RNA dual-labeling method would have widespread applications for preparation of functionalized RNA beacons.…”
Section: Discussionmentioning
confidence: 99%
“…On the other hand, the dual-terminal labeling strategy should be useful for large-scale and facile production of any RNA beacon in principle. RNA beacons can be used for miRNA detection, visualization of microRNA maturation, specific RNA imaging, separation of cell populations, and identification of inhibitors of RNA-binding protein [43][44][45][46][47][48] . Therefore, it is anticipated that the RNA dual-labeling method would have widespread applications for preparation of functionalized RNA beacons.…”
Section: Discussionmentioning
confidence: 99%
“…Another method commonly used for labelling of intracellular RNA are molecular beacons (MB) [ 57 ]. These nanodevices contain a target sequence flanked by self-complimentary palindromic sequences which form a stem-loop ( Figure 7 A) [ 57 , 58 ]. Typically, the 3′ and 5′ ends of the MB are conjugated with a fluorophore and quencher, respectively.…”
Section: Recent Developments In Dna and Mrna Detection For Living Cellsmentioning
confidence: 99%
“…Upon hybridisation with the target sequence, the fluorophore and quencher are distanced from each other, resulting in fluorescence emission ( Figure 7 B). An advantage over aptamer-based labelling is that MBs do not require the engineering of the virus and generally exhibit low noise [ 58 ], although imperfect quenching of the fluorophore or degradation by nucleases may still result in background fluorescence [ 57 ]. Furthermore, MBs are prone to non-specific protein binding, which can lead to non-specific signals.…”
Section: Recent Developments In Dna and Mrna Detection For Living Cellsmentioning
confidence: 99%
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