Recent advances in the rapid detection of microRNA with lateral flow assays

Wang, Nan; Zhang, Juan; Xiao, Bin; Sun, Xiaoyun; Xie, Ruibin; Chen, Ailiang

doi:10.1016/j.bios.2022.114345

Cited by 41 publications

(31 citation statements)

References 94 publications

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“…The classic bioreceptors used in LF tests are antibodies (Ab), but other bioreceptors such DNA probes ,− or aptamers − can be integrated depending on the target to be detected.…”

Section: Lateral Flowmentioning

confidence: 99%

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Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

2022

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“…The classic bioreceptors used in LF tests are antibodies (Ab), but other bioreceptors such DNA probes ,− or aptamers − can be integrated depending on the target to be detected.…”

Section: Lateral Flowmentioning

confidence: 99%

“…Each of these techniques have their advantages and disadvantages but all of them are far from being POCT platforms. This unmet need could be covered by isothermal amplification assays and RDT based on Lateral Flow (LF), which hold potential as POCT systems for miRNA detection. − …”

Section: Introductionmentioning

confidence: 99%

Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

2022

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“…7,8 For better miRNA detection, nanoparticle-based nucleic acid probes have been extensively applied to design a LFTS. 9,10 Normally, gold nanoparticles (AuNPs) were deployed for the construction of a LFTS to show a colorimetric signal from the AuNP−DNA−miRNA−DNA sandwich complexes that accumulate at the test zone for qualitative and quantitative analysis. 11 However, for the detection of miRNA in biological samples, the AuNP-based LFTS still needs to undergo complex RNA extraction and nucleic acid amplification processes for augmented sensitivity.…”

mentioning

confidence: 99%

“…Lateral flow test strip (LFTS) technology, also known as lateral flow immunoassay, exhibited gratifying promise for the direct point-of-care testing (POCT) of disease biomarkers, ascribed to its equipment-free, user-friendly, and cost-effective manners . The typical LFTS combined immunoassays and chromatographic analysis, which was widely used for predicting pregnancy, detection of disease biomarkers, inspection of food safety, etc. , For better miRNA detection, nanoparticle-based nucleic acid probes have been extensively applied to design a LFTS. , Normally, gold nanoparticles (AuNPs) were deployed for the construction of a LFTS to show a colorimetric signal from the AuNP–DNA–miRNA–DNA sandwich complexes that accumulate at the test zone for qualitative and quantitative analysis . However, for the detection of miRNA in biological samples, the AuNP-based LFTS still needs to undergo complex RNA extraction and nucleic acid amplification processes for augmented sensitivity. , These complex processes are labor-intensive and time-consuming and may hamper the overall sensing sensitivity.…”

mentioning

confidence: 99%

Dual-Modal Lateral Flow Test Strip Assisted by Near-Infrared-Powered Nanomotors for Direct Quantitative Detection of Circulating MicroRNA Biomarkers from Serum

Chen

Xing

et al. 2023

ACS Sens.

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Quantification of microRNA (miRNA) has attracted intense interest owing to its importance as a biomarker for the early diagnosis of multiple diseases. However, the inefficient capture of microRNAs from complex biological samples due to the passive diffusion of detection probes essentially restricts their accurate quantification. Herein, we report near-infrared (NIR)-powered Janus nanomotors composed of Au nanorods and periodic mesoporous organo-silica microspheres (AuNR/PMO JNMs) as “swimming probes” to assist a lateral flow test strip (LFTS) for direct, amplification-free, and quantitative miRNA-21 detection in serum and cell medium. The AuNR/PMO JNMs were conjugated with designed hDNA as a recognition probe for miRNA-21. Under NIR irradiation, the exposed AuNRs can generate asymmetric thermal gradients around the JNMs to achieve vigorous self-propelled thermophoretic motion. The active movement significantly accelerated the recognition of miRNA-21 targets, which greatly improved the capture efficiency from 59.39 to 86.12% in the reaction buffer. The enhanced miRNA-21 capture enabled direct quantitative miRNA-21 detection on the LFTS assay with both visual and thermal signals. Under the assistance of AuNR/PMO JNMs, a limit-of-detection of 18 fmol/L for miRNA-21 was achieved, which was 12.22-fold compared to that of LFTS assay with static probes. The constructed LFTS assay was further successfully deployed to directly sense the miRNA-21 in spiked serum samples and MDA-MB-231 medium. Overall, the AuNR/PMO JNM-assisted LFTS system unveils a concrete point-of-care testing strategy for precise miRNA detection in real biological samples, which holds great potential for early diagnosis and treatment of miRNA-related diseases.

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“…Thus, the development of a simple and field-deployable point-of-care (POC) testing system for the rapid detection of miRNAs is meaningful and essential. Currently, the POC detection method based on lateral flow strips has been a promising approach for the processing and sensing of miRNAs. , Despite the practicality of the test strip, the sensitivity limits their use in low abundance miRNA detection. Up to now, a variety of DNA nanotechnology-based isothermal amplification strategies have been developed for bioanalysis, such as enzyme-assisted methods − and enzyme-free assays .…”

mentioning

confidence: 99%

Dual-AND Logic Gate-Based Strip Assay for Amplified Detection of Four miRNAs and Diagnosis of Lung Cancer

Zhou

Pan

et al. 2022

Anal. Chem.

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The detection of circulating tumor microRNAs (miRNAs) holds great promise for the noninvasive and early-stage diagnosis of cancer. However, the low abundance of lung cancer-related miRNAs and the false-positive results of single miRNA detection limited the development of strip-based point-of-care testing methods in clinic. We developed a duplex-specific nuclease (DSN)-mediated and dual-AND logic gate-based triple-line lateral flow strip detection system for the rapid and simultaneous detection of four miRNAs of lung cancer in a single strip test. This system combines DSN-mediated signal amplification with AND logic gate-based simple signal output. Meanwhile, the limit of detection of this platform was calculated to be 26.51 fM. Furthermore, this assay was used to detect lung cancer-related miRNAs from serum in a homogeneous and separation-free format, which could discriminate lung cancer patients from healthy individuals with an accuracy of 100%. Our approach provides a simple and easy-to-handle method for the diagnosis of lung cancer in clinic.

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Recent advances in the rapid detection of microRNA with lateral flow assays

Cited by 41 publications

References 94 publications

Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

Toward Integrated Molecular Lateral Flow Diagnostic Tests Using Advanced Micro- and Nanotechnology

Dual-Modal Lateral Flow Test Strip Assisted by Near-Infrared-Powered Nanomotors for Direct Quantitative Detection of Circulating MicroRNA Biomarkers from Serum

Dual-AND Logic Gate-Based Strip Assay for Amplified Detection of Four miRNAs and Diagnosis of Lung Cancer

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