Recent advances in time resolved circular dichroism spectroscopy

Kliger, David S.; Lewis, James W.

doi:10.1007/bf03055509

Cited by 26 publications

(12 citation statements)

References 40 publications

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“…These spacings are in agreement with those measured from IR and Raman spectra. 16,11 The presence of two progressions in the (12) Hipps, K. W.; Francis, A. H. J. Phys. Chem.…”

Section: Resultsmentioning

confidence: 99%

“…Our laboratory has developed a method for determining the CD spectra of short-lived molecular species, and it has been shown to be useful in obtaining CD spectra with nanosecond time resolution. 10,11 Using this time-resolved circular dichroism (TRCD) method, we have previously reported the CD spectrum of the excited-state absorptions of (A)-Ru(bpy)32+ and (A)-Fe-(bpy)32+.12™14…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The emission spectrum of ruthenocene: calculation of the excited-state distortions and the spacings in the repetitive pattern

Hollingsworth¹,

Shin²,

Zink³

1990

Inorg. Chem.

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“…These spacings are in agreement with those measured from IR and Raman spectra. 16,11 The presence of two progressions in the (12) Hipps, K. W.; Francis, A. H. J. Phys. Chem.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The emission spectrum of ruthenocene: calculation of the excited-state distortions and the spacings in the repetitive pattern

Hollingsworth¹,

Shin²,

Zink³

1990

Inorg. Chem.

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“…TRCD can provide a unique method for measuring structural rearrangement and folding for both smaller chiral species and large macromolecules in different environments. Recent advances in measuring TRCD have provided excitement about the possibilities and power of such measurements. − More conventional TRCD methods are limited by the time scale of the polarization modulator employed. While others can use shorter-pulse lasers, they often depend on the inherently weak difference between two large absorption signals or are ellipsometric and are subject to significant optical artifacts …”

Section: Discussionmentioning

confidence: 99%

Circular Dichroism Spectroscopy Using Coherent Laser-Induced Thermal Gratings

et al. 1997

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A new pulsed four-wave mixing technique for the detection and real-time measurement of circular dichroism (CD) in liquid samples is demonstrated. The technique is based on the formation and detection of transient thermal gratings formed by the interference of two laser beams whose polarizations are controlled and modulated using a photoelastic modulator. Through an internal heterodyne process, coherent thermal gratings interfere to greatly enhance a weak circular dichroism signal. By measuring the ratio of the difference of scattered laser light from two different polarizations, ΔS, to the average amount of scattered light, S ave, one can determine the value of Δε/ε for the compound under investigation. Samples of chiral camphorquinone are used to demonstrate the technique in the ultraviolet region of the spectrum. These studies of camphorquinone, which has a value of Δε/ε ≈ 10-3 at 266 nm, produce values of ΔS/S ave which are orders of magnitude larger than Δε/ε and which approach 2 (the mathematical limit for ΔS/S ave). Possible extensions of the technique for measuring CD in very small sample volumes and monitoring time-dependence are discussed.

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“…Time-resolved absorption (TROD) measurements were performed as described previously (35,36). A xenon flashlamp produced probe pulses at 1 Hz which are focused on the sample to a diameter of about 8 mm.…”

Section: Methodsmentioning

confidence: 99%

Carbon Monoxide Religation Kinetics to Hemoglobin S Polymers following Ligand Photolysis

Shapiro

Esquerra

Goldbeck

et al. 1995

Journal of Biological Chemistry

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The re-equilibration rate of carbon monoxide binding to hemoglobin S polymers is determined by time-resolved measurements of linear dichroism spectra. Linear dichroism is used to detect religation to hemoglobin in the polymer in the presence of rebinding to free hemoglobin S tetramers. Measurement of the linear dichroism resulting from photolysis of the small percentage of ligand bound to the polymer is accomplished through the use of an ultrasensitive, ellipsometric linear dichroism technique developed for this purpose. The major finding is that the return of the polymer phase to its equilibrium ligation state is much slower than that of the solution phase hemoglobin tetramers. Assuming all hemes in the polymer are equally likely to participate in rebinding, the re-equilibration rate for carbon monoxide religation to hemoglobin S polymers is found to be 0.14 ؎ 0.07 (s ؊1 mM ؊1 ), about 1000 times slower than the rebinding rate of carbon monoxide to T-state monomer hemoglobin. Several interpretations of this result are discussed. An understanding of the ligand binding kinetics to hemoglobin S polymers could have pathophysiological significance in its relevance to polymer formation and melting during red blood cell circulation.

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Recent advances in time resolved circular dichroism spectroscopy

Cited by 26 publications

References 40 publications

The emission spectrum of ruthenocene: calculation of the excited-state distortions and the spacings in the repetitive pattern

The emission spectrum of ruthenocene: calculation of the excited-state distortions and the spacings in the repetitive pattern

Circular Dichroism Spectroscopy Using Coherent Laser-Induced Thermal Gratings

Carbon Monoxide Religation Kinetics to Hemoglobin S Polymers following Ligand Photolysis

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