Recent advances in utilizing bacteria as biocontrol agents against plant parasitic nematodes emphasizing Meloidogyne spp.

Antil, Sonam; Kumar, Rohtash; Pathak, D. V.; Kumari, Anju

doi:10.1016/j.biocontrol.2023.105244

Cited by 25 publications

(4 citation statements)

References 106 publications

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“…To date, to our knowledge, this is the first report that confirms the presence of NRPS genes in strains of Bacillus tropicus , Bacillus velenzensis , Bacillus proteolyticus , and Bacillus paramycoides . B. velezensis is known to be a lipopeptide producer, i.e., B. velezensis FZB2 is known to be a PGPR ( Antil et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

et al. 2023

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Bacillus species and their metabolites have potential alternative uses as chemical pesticides that can limit the growth of potential plant pathogens and enhance crop productivity. The aim of this study was to investigate the potential of Bacillus subtilis FJ3 for promoting plant growth and controlling fungal plant pathogens. The study evaluated the ability of the strain to promote plant growth in vitro by characterizing its growth-promoting traits, which included the production of hydrolytic enzymes, indole acetic acid, siderophores, biofilm formation, and phosphate solubilization. Polymerase Chain Reaction (PCR) testing revealed that strain FJ3 has the potential to produce lipopeptides such as fengycin, surfactin, mycosubtilin, and pilpastatin. Through in vitro antagonism testing it was demonstrated that strain FJ3 is able to inhibit Fusarium oxysporum by 52% compared to the untreated control and was antagonistic against Aspergillus flavus, Aspergillus niger, and Rhizopus oryzae using a dual method. The minimum inhibitory concentration of Bacillus crude extract resulted in a 92%, 90%, 81.5%, and 56% growth inhibition of Fusarium oxysporum, A. niger, A. flavus, and Rhizopus oryzae, respectively. In FT-IR and GC-MS analysis of crude LPs extract, the transmission and mass spectrum confirmed the existence of aforesaid lipopeptides containing β-fatty acids with chain lengths ranging from C14 to C21 in which the majority were saturated fatty acids. Greenhouse experimentation revealed that Bacillus strain FJ3 and its metabolites significantly diminished the disease incidence with an average reduction of 31.56%. In sterilized soil, FJ3 and its metabolites caused 24.01% and 10.46% growth promotion, respectively, in chickpea. The results demonstrated that Bacillus strain FJ3 has broad-spectrum antifungal and plant growth-promoting applications and could be a promising candidate for development into a commercialized biobased product for use in sustainable agriculture practice.

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Section: Discussionmentioning

confidence: 99%

In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

et al. 2023

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“…In addition, eight of them lacked β-hemolytic activity, including isolates with high inhibitory activity against multidrug-resistant (MDR) bacteria (B. laterosporus) and isolates with activity against fungi (B. pumilus and B. altitudinis), which makes them promising candidates for potential future applications. The members of Bacillus and Brevibacillus genera obtained in this research are known for their ability to produce a variety of bioactive compounds with different activities such as antibacterial [29,30], antifungal [16,[31][32][33][34][35][36][37][38], antiviral [39][40][41], and pest control properties [42,43].…”

Section: Discussionmentioning

confidence: 99%

Searching for Antimicrobial-Producing Bacteria from Soils through an Educational Project and Their Evaluation as Potential Biocontrol Agents

Pino-Hurtado,

Fernández-Fernández,

Torres

et al. 2023

Antibiotics

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Antimicrobial resistance (AMR) is a serious threat to public health due to the lack of effective drugs to combat infectious diseases, which generates the need to search for new antimicrobial substances. In this study, the potential of soil as a source of antimicrobial-producing bacteria (APB) was investigated and the importance of the connection between education and science was emphasized, using service-learning methodologies. Sixty-one soil samples were collected, and 1220 bacterial isolates were recovered. Eighteen of these isolates showed antimicrobial activity against at least 1 of the 12 indicator bacteria tested (including multidrug-resistant and relevant pathogens). The 18 APB were identified by MALDI-TOF and 6 different genera (Bacillus, Brevibacillus, Lysinobacillus, Peribacillus, Streptomyces, and Advenella) and 10 species were identified. The 18 APB were tested for antifungal activity against four phytopathogenic fungi (Botritis cynerea, Lecanicillium fungicola, Trichoderma harzianum, and Cladobotryum mycophilum). Moreover, the antibiotic susceptibility of APB was tested using the disk-diffusion method as well as their β-hemolytic activity (important safety criteria for potential future applications). A total of 10 of the 18 APB were able to inhibit at least 50% of indicator bacteria tested, including methicillin-resistant Staphylococcus aureus (MRSA), among others. A total of 4 of the 18 APB (3 Bacillus pumilus and 1 Bacillus altitudinis) showed inhibitory activity against two of the four fungal pathogens tested (B. cinerea and L. fungicola), as well as against 5–7 of the 12 bacterial pathogen indicators; these 4 isolates showed susceptibility to the antibiotics tested and lacked β-hemolytic activity and were considered promising APB for use as potential biocontrol agents. In addition, one Brevibacillus laterosporus strain had activity against 83% of indicator bacteria tested including Escherichia coli, MRSA and other methicillin-resistant staphylococci, as well as vancomycin-resistant enterococci (but not against fungi). These results show that soil is a source of APB with relevant antibacterial and antifungal activities, and also emphasize the importance of education and science to raise public awareness of the AMR problem and the strategies to control it.

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“…Por otra parte, las palabras claves más recientes son metabolómica, transcriptómica, traducción de señales, mecanismos de defensas, estrés biótico y abiótico y agricultura. Actualmente se emplean diversas técnicas mediante la metagenómica y la metabolómica, con el fin de identificar bacterias, hongos y una gama de biomoléculas con fuertes propiedades inhibidoras del crecimiento de nematodos (Antil et al, 2023;. No obstante, la ausencia de herramientas, técnicas y estandarización a través de enfoques multiómicos para microbiomas de pequeña escala ha restringido el avance y la comprensión de la diversidad completa de las interacciones microbianas (Kelliher et al, 2023).…”

Section: Desafíos Actuales Y Futurosunclassified

Bioactive compounds from bacterial and fungal agents for the control of phytopathogenic nematodes: mechanisms of action, interactions, and applications

Vera-Morales,

Castañeda-Ruiz,

Sosa

et al. 2024

Sci. agropecu.

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Phytopathogenic nematodes are a threat for agriculture, they cause plant diseases and economic losses worldwide. Currently, the pursuit of compounds of biological origin for nematode biocontrol has become a priority as an environmentally friendly alternative. Microorganisms-derived compounds have several strategies to reduce the populations of infectious juvenile nematodes. Although microorganisms and their metabolites are the focus of literature reports reviews on nematicidal control, interactions between fungi, bacteria, and nematodes, as well as the compounds that are generated in such interactions, are not highlighted. The aim of this review was to discuss the latest advances on chemical compounds secreted by microorganisms and their interaction mechanisms for biological control of phytopathogenic nematodes. Results were organized based on: i) mode of action, ii) infection process, iii) nematicide compounds, iv) interactions and field applications, and v) current challenges. The scope of this review contributes to a better comprehension of the versatility of bacterial and fungal compounds for their use in the development of management plans for nematode control.

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Recent advances in utilizing bacteria as biocontrol agents against plant parasitic nematodes emphasizing Meloidogyne spp.

Cited by 25 publications

References 106 publications

In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

In vitro assessment of Bacillus subtilis FJ3 affirms its biocontrol and plant growth promoting potential

Searching for Antimicrobial-Producing Bacteria from Soils through an Educational Project and Their Evaluation as Potential Biocontrol Agents

Bioactive compounds from bacterial and fungal agents for the control of phytopathogenic nematodes: mechanisms of action, interactions, and applications

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