Recent Advances of DNA Nanostructure‐Based Cell Membrane Engineering

Feng, Lingyu; Li, Jiang; Sun, Jingyong; Wang, Lihua; Fan, Chunhai

doi:10.1002/adhm.202001718

Cited by 31 publications

(26 citation statements)

References 150 publications

(269 reference statements)

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“…Stability of nucleic acids is one of the main issues of concern. Both DNA and RNA are swiftly degraded in the presence of nuclease enzymes; however, nucleic acid nanostructures have been successfully redesigned and developed specifically to minimize degradation by nucleases. , Additionally, chemical modifications on nucleic acid nanostructures enhancing nuclease resistance activity have been developed. ,, For freedom of design, DNA nanostructures have been created with a wide range of architectures, in arbitrary shapes (from rigid to wireframe structures) and many length scales (from nano- to micrometer size) that are versatile and provide larger and more sophisticated designs than RNA or DNA/RNA hybrid nanostructures. ,,− Computer-aided design software is crucial for spreading design skills and experience to a wider community and for moving the field forward. User-friendly semiautomated and automated software tools for designing DNA nanostructures, in particular DNA origami have been extensively developed and are available online. ,,− However, software customized for designing RNA and DNA/RNA hybrid materials are, thus far, limited.…”

Section: Discussionmentioning

confidence: 99%

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

et al. 2021

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Researchers have worked for many decades to master the rules of biomolecular design that would allow artificial biopolymer complexes to self-assemble and function similarly to the diverse biochemical constructs displayed in natural biological systems. The rules of nucleic acid assembly (dominated by Watson–Crick base-pairing) have been less difficult to understand and manipulate than the more complicated rules of protein folding. Therefore, nucleic acid nanotechnology has advanced more quickly than de novo protein design, and recent years have seen amazing progress in DNA and RNA design. By combining structural motifs with aptamers that act as affinity handles and add powerful molecular recognition capabilities, nucleic acid-based self-assemblies represent a diverse toolbox for use by bioengineers to create molecules with potentially revolutionary biological activities. In this review, we focus on the development of self-assembling nucleic acid nanostructures that are functionalized with nucleic acid aptamers and their great potential in wide ranging application areas.

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Section: Discussionmentioning

confidence: 99%

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

et al. 2021

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“…Its size and shape can be programed to arrange biological recognition sites. [1] These properties make DNA widely used in cell membrane engineering. It is expected to develop powerful, intelligent, versatile and programmable nanodevices to regulate cell activities.…”

Section: Non-specific Modification Methodsmentioning

confidence: 99%

Recent Progress and Perspectives on Cell Surface Modification

Zhang

Zhou

Qian

2021

Chemistry An Asian Journal

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The cell membrane is a biological interface consisting of phospholipid bilayer, saccharides and proteins that maintains a stable metabolic intracellular environment as well as regulating and controlling the exchange of substances inside and outside the cell. Cell membranes provide a highly complex biological surface carrying a variety of essential surfaces ligands and receptors for cells to receive various stimuli of external signals, thereby inducing corresponding cell responses regulating the life activities of the cell. These surface receptors can be manipulated via cell surface modification to regulate cellular functions and behaviors Thus, cell surface modification has attracted considerable attention due to its significance in cell fate control, cell engineering and cell therapy. In this minireview, we describe the recent developments and advances of cell surface modification, and summarize the main modification methods with corresponding functions and applications. Finally, the prospect for the future development of the modification of the living cell membrane is discussed.

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“…Meanwhile, intensive efforts have been made by chemical and biomedical communities to develop alternative strategies for cell-surface engineering [6]. DNA, taking advantage of easy synthesis, convenient modification, high programmability, and good biocompatibility, has become one of the most promising materials in this regard [7][8][9][10]. In addition to being the carrier of genetic information, DNA, with specific biological and chemical functions, carrier of genetic information, DNA, with specific biological and chemical functions, including aptamers, DNAzyme, and molecular beacons, has been developed and attracted a broad interest for both biological and chemical research [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…Soon after the pioneered work of Nedrian C. Seeman, [25] many DNA nanostructures, including DNA origami, DNA framework, and dynamic DNA network, have been reported [26][27][28][29][30][31]. These DNA nanostructures showed multiple merits for cell membrane engineering [9], including the capability of controlling the structure and function on a nano-scale and high programmability and addressability for precise biological mimicry and manipulation [32,33]. In this review, we summarized recent progress in exploiting nucleic acids for cell membrane engineering (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

DNA-Based Molecular Engineering of the Cell Membrane

Wang

Sun

et al. 2022

Membranes

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The cell membrane serves as a barrier and gatekeeper to regulate the cellular transportation of substances and information. It plays a significant role in protecting the cell from the extracellular environment, maintaining intracellular homeostasis, and regulating cellular function and behaviors. The capability to engineer the cell membrane with functional modules that enable dynamic monitoring and manipulating the cell-surface microenvironment would be critical for studying molecular mechanisms underlying various biological processes. To meet this goal, DNA, with intrinsic advantages of high versatility, programmability, and biocompatibility, has gained intense attention as a molecular tool for cell-surface engineering. The past three decades have witnessed the rapid advances of diverse nucleic acid materials, including functional nucleic acids (FNAs), dynamic DNA circuits, and exquisite DNA nanostructures. In this mini review, we have summarized the recent progress of DNA technology for cell membrane engineering, particularly focused on their applications for molecular sensing and imaging, precise cell identification, receptor activity regulation, and artificial membrane structures. Furthermore, we discussed the challenge and outlook on using nucleic acid materials in this specific research area.

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Recent Advances of DNA Nanostructure‐Based Cell Membrane Engineering

Cited by 31 publications

References 150 publications

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

Self-Assembling Nucleic Acid Nanostructures Functionalized with Aptamers

Recent Progress and Perspectives on Cell Surface Modification

DNA-Based Molecular Engineering of the Cell Membrane

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