Recent advances of immunosensors for detecting food allergens

Hosu, Oana; Selvolini, Giulia; Marrazza, Giovanna

doi:10.1016/j.coelec.2018.05.022

Cited by 41 publications

(15 citation statements)

References 46 publications

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“…Currently, a wide range of analytical techniques has been integrated for the development of multiplexed immunosensing systems for cancer biomarkers. Electrochemical immunosensors have received great interest due to their high sensitivity provided by coupling the immunochemical affinity reaction (antigen-antibody) with the particular features of multifunctional electrode transduction elements [41,47].…”

Section: Biosensingmentioning

confidence: 99%

Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment

2019

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During the last few decades, magnetic nanoparticles have been evaluated as promising materials in the field of cancer detection, screening, and treatment. Early diagnosis and screening of cancer may be achieved using magnetic nanoparticles either within the magnetic resonance imaging technique and/or sensing systems. These sensors are designed to selectively detect specific biomarkers, compounds that can be related to the onset or evolution of cancer, during and after the treatment of this widespread disease. Some of the particular properties of magnetic nanoparticles are extensively exploited in cancer therapy as drug delivery agents to selectively target the envisaged location by tailored in vivo manipulation using an external magnetic field. Furthermore, individualized treatment with antineoplastic drugs may be combined with magnetic resonance imaging to achieve an efficient therapy. This review summarizes the studies about the implications of magnetic nanoparticles in cancer diagnosis, treatment and drug delivery as well as prospects for future development and challenges of magnetic nanoparticles in the field of oncology.

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Section: Biosensingmentioning

confidence: 99%

Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment

2019

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“…Di-sodium hydrogen phosphate (Na 2 HPO 4 ), sodium dihydrogenphosphate dihydrate (NaH 2 PO 4 ·2H 2 O), sodium chloride (NaCl), L-lysine hydrochloride (C 6 H 14 N 2 O 2 ·HCl), tetrachloroauric acid (HAuCl 4 ), sulfuric acid (H 2 SO 4 ), and potassium chloride (KCl) were acquired from Merck (Milan, Italy). Potassium hexacyanoferrate (II) trihydrate (K 4 [Fe(CN) 6 ]·3H 2 O), potassium hexacyanoferrate (III) (K 3 [Fe(CN) 6 ]), Trizma ® Base (Tris), magnesium chloride hexahydrate (MgCl 2 ·6H 2 O), β-lactoglobulin B (β-LG), and 6-mercapto-1-hexanol (MCH) were obtained from Sigma-Aldrich (Milan, Italy).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…Nowadays, allergy to food is a very important global health problem, thus an emerging topic when talking about food safety [1,2]. Allergy to food has been recently defined by the European Academy of Allergology and Clinical Immunology [3] and it involves an immune reaction deriving from the intake or other type of interaction with specific food [4,5]. Food allergy is a type I hypersensitivity immunological response against ingested substances that can result in serious health problems [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Although these methods are very sensitive, they are time-consuming and the instrumentation needed is very expensive. Because of this, there is an increasing interest in developing new analytical tools which are faster, simpler and with a lower-cost, also keeping good analytical characteristics [3,30]. Hence, electrochemical biosensors satisfy all these requirements, and, in particular, aptamer-based sensors (also known as aptasensors) are listed as one of the most powerful type of biosensors [31].…”

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confidence: 99%

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Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes

Amor-Gutiérrez

Selvolini

Fernández‐Abedul

et al. 2020

Sensors

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Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because of this, the development of point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become of outstanding importance. According to this, electrochemical aptasensors have been demonstrated as useful tools to quantify a broad variety of targets. In this work, we develop a simple methodology for the determination of β-lactoglobulin (β-LG) in food samples using a folding-based electrochemical aptasensor built on poly-L-lysine modified graphite screen-printed electrodes (GSPEs) and an anti-β-lactoglobulin aptamer tagged with methylene blue (MB). This aptamer changes its conformation when the sample contains β-LG, and due to this, the spacing between MB and the electrode surface (and therefore the electron transfer efficiency) also changes. The response of this biosensor was linear for concentrations of β-LG within the range 0.1-10 ng·mL −1 , with a limit of detection of 0.09 ng·mL −1 . The biosensor was satisfactorily employed for the determination of spiked β-LG in real food samples.incidence of the most popular food allergies throughout Europe [13] revealed that allergy to cow's milk is, nowadays, the most frequent food allergy, especially in early-age children, implying a percentage of around 6% in the youngest population [14,15]. Immunoglobulin E (IgE) normally mediates cow's milk allergy, and the symptoms usually occur within 2 h after milk intake [16]. In whey from cow's milk, one of the most important proteins considered to be an allergen is β-lactoglobulin (β-LG), which is classified within the lipocalins, a group of binding proteins with small ligands [17]. Its quaternary structure, identified in 1997 using X-Ray diffraction [18], is dependent on the pH. For example, at pH values between 5.2 and 7.0, it is a dimer, and its molecular weight (MW) is around 37 kDa. However, at pH values over 8.0, it exists as a monomer, and its MW is approximately 18 kDa [19]. This protein has a great potential as milk allergen because it is remarkably stable and it is not present in human milk [20], and, apart from that, it is regularly used as an additive in lots of food products [21].The development of analytical approaches used to detect and quantify food allergens has become increasingly significant [22] because labeling in food is crucial for the consumers, in order to prevent major health problems. The analysis of allergens in food samples [23] is usually carried out by immunoassay-based approaches, such as enzyme-linked immunosorbent assays (ELISAs) [11,14,24] or lateral-flow immunoassays (LFIAs) [25,26], as well as DNA-based methods, which detect the genes encoding for the proteins using amplifying methods, for instance, the polymerase chain reaction (PCR) [27,28]. Proteomic tests have been also used for the determination of allergens [29]. Although these methods are very sensitive, they are time-consuming and the instrumenta...

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“…For these reasons, the development of an easy-to-use, fast and accurate device for allergen detection is fundamental to prevent life-threatening reactions in allergic people. In this context, biosensors are a valid alternative to conventional detection methods and they have attracted considerable interest in the last decades [35][36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer

et al. 2019

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In this work, we present a new electrochemical disposable platform based on poly(aniline‐co‐anthranilic acid) (PANI/PAA) composite polymer coupled with an aptamer for sensitive detection of β‐lactoglobulin. Firstly, PANI/PAA film was electrodeposited on the graphite screen‐printed electrode surface by cyclic voltammetry. The co‐polymer modified electrode was then employed as platform for the covalent immobilization of an amino‐modified aptamer. Various β‐lactoglobulin solutions, with a fixed amount of biotinylated oligonucleotide complementary sequence, were dropped onto the aptasensor surface. A streptavidin‐alkaline phosphatase conjugate was then employed to trace the affinity reaction. After the addition of 1‐naphthyl‐phosphate enzymatic substrate, 1‐naphthol electroactive product was detected by differential pulse voltammetry. A decrease in the signal was obtained when the target concentration was increased, in according to a signal‐off approach. After optimization of key experimental parameters, a dose‐response curve was obtained between 0.01–1.0 μg mL−1 β‐lactoglobulin concentration range. The limit of detection of 0.053 μg L−1 was obtained. Milk samples spiked with β‐lactoglobulin were analyzed.

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Recent advances of immunosensors for detecting food allergens

Cited by 41 publications

References 46 publications

Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment

Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment

Folding-Based Electrochemical Aptasensor for the Determination of β-Lactoglobulin on Poly-L-Lysine Modified Graphite Electrodes

Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer

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