Recent advances of Raman spectroscopy for the analysis of bacteria

Abstract

Rapid and sensitive bacteria detection and identification are becoming increasingly important for a wide range of areas including the control of food safety, the prevention of infectious diseases, and environmental monitoring. Raman spectroscopy is an emerging technology which provides comprehensive information for the analysis of bacteria in a short time and with high sensitivity. Raman spectroscopy offers many advantages including relatively simple operation, non‐destructive analysis, and information on molecular differences between bacteria species and strains. A variety of biochemical properties can be measured in a single spectrum. This short review covers the recent advancements and applications of Raman spectroscopy for bacteria analysis with specific focuses on bacteria detection, bacteria identification and discrimination, as well as bacteria antibiotic susceptibility testing in 2022. The development of novel substrates, the combination with other techniques, and the utilization of advanced data processing tools for the improvement of Raman spectroscopy and future directions are discussed.

Advances in Nanoarchitectonics: A Review of “Static” and “Dynamic” Particle Assembly Methods

“…Nanoparticle arrays hold promising applications across diverse fields. They can serve as sophisticated photonic structures, finding utility in structural coloration and advanced optical biosensing [ 70 , 74 , 75 ]. Furthermore, they offer the potential to develop electronic nano-devices and chemical/biological sensors.…”

“…Table 1 provides the advantages and disadvantages of each assembly technique studied in this review. Static methods like layer-by-layer assembly [ 51 , 52 , 53 , 58 , 59 , 60 , 61 , 62 ] and template-assisted assembly [ 2 , 10 , 16 , 29 , 70 , 72 , 73 , 74 , 75 ] may need specific substrates. Polymer brushes [ 83 , 84 , 85 , 86 ] offer adjustability but risk aggregation.…”

Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms

“…Since Raman spectroscopy always samples all biomolecules inside the probing volume, most Raman methods are phenotypic which means the information of all biomolecules can be found in the Raman spectra of bacteria [ 15 , 16 , 17 , 18 , 19 ]. In principle, there are two types of Raman measurements of bacteria—bulk samples and single-cell measurements.…”

Unveiling the efficacy of a bulk Raman spectra-based model in predicting single cell Raman spectra of microorganisms

“…In a recent review, Rodriguez et al [ 40 ] conclude that the use of advanced data processing methods will allow for a better interpretation of Raman spectra and the detection of minor differences in biological samples. Since it might be a problem to compare the results obtained from different data processing programs, future work should address this problem by developing a universal tool or database for more reliable results [ 40 ]. In our recent studies we showed that differentiation of bacteria and yeasts after standardized growth conditions using microbial bulk material for spectral recording was possible [ 11 ].…”