Recent advances of transient isotachophoresis‐capillary electrophoresis in the analysis of small ions from high‐conductivity matrices

Timerbaev, Andrei R.; Hirokawa, Takatsugu

doi:10.1002/elps.200500320

Cited by 124 publications

(121 citation statements)

References 110 publications

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“…In addition to the observed migration time shift, a slight increase in plate number of the peaks for the high-ionic-strength samples (non-desalted MeOH and ultrafiltration extracts) was obtained. This effect is contrary to the expected from theory (higher electromigration dispersion in the more salty samples) and although at the moment we do not see a clear reason, it could be explained through a stacking process induced by the sodium clusters band mentioned above [16].…”

Section: 31matrix Effectcontrasting

confidence: 99%

Is metabolomics reachable? Different purification strategies of human colon cancer cells provide different CE‐MS metabolite profiles

et al. 2011

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In this work, four different metabolite purification approaches are investigated prior to metabolomics of human HT29 colon cancer cells. Namely, methanol deproteinization, ultrafiltration and two SPE methods using C18 and polymer‐based cartridges were studied. The extracts were characterized via a metabolomic approach based on the application of CE TOF MS (CE‐MS). CE‐MS analysis time was less than 20 min per sample and allowed the simultaneous and reproducible analysis of more than 80 metabolites in a single run with a minimum consumption of sample and reagents. Metabolome analysis revealed in some cases important differences among the studied metabolite purification procedures. No significant differences were observed in the metabolite profile using C18 and polymer‐based cartridges, or between ultrafiltration and methanol deproteinization. However, important differences were observed in the metabolomic profiles obtained from SPE and methanol deproteinization samples. These results demonstrate the crucial role of the metabolite purification strategy in metabolomics since it can bias (and in some cases mislead) the conclusions achieved by the metabolomic study.

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Section: 31matrix Effectcontrasting

confidence: 99%

Is metabolomics reachable? Different purification strategies of human colon cancer cells provide different CE‐MS metabolite profiles

et al. 2011

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“…TITP is a technique in which solutes are initially concentrated by ITP and then separated by capillary zone electrophoresis (CZE) in a single capillary. [11][12][13][14] In other words, a heterogeneous system of electrolytes satisfying Eq. (2) exists transiently, followed by the restoration of a homogeneous BGE system for CZE.…”

Section: +mentioning

confidence: 99%

Isotachophoretically Assisted On-Line Complexation of Trace Metal Ions in a Highly Saline Matrix for Capillary Electrophoresis

Kim¹,

Choi²,

Cho³

et al. 2012

Bulletin of the Korean Chemical Society

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Trace metal ions such as Cd 2+, Ni 2+, and Zn 2+ in a highly saline sample were subjected to on-line complexation with 4-(2-thiazolylazo) resorcinol (TAR) dissolved in a background electrolyte (BGE) under transient isotachophoresis (TITP) conditions. A long plug of the saline sample, containing the trace metal ions but devoid of TAR, was injected into a coated capillary filled with a BGE composed of 150 mM 2-(cyclohexylamino) ethanesulfonic acid (CHES) and 110 mM triethylamine (TEA) at pH 9.7. Since the electrophoretic mobility of TAR fell between the mobilities of the anionic leading electrolyte (Cl − in the sample) and the anionic terminating background electrolyte (CHES − ), a highly concentrated zone of TAR from the BGE was formed at the rear of the sample matrix and then the metal cations toward the cathode were swept by isotachophoretically assisted on-line complexation (IAOC) between the metal ions and the isotachophoretically stacked TAR. As a result, anionic metal-TAR complexes were formed efficiently, which satisfy the TITP conditions between Cl − and CHES − . The enrichment factors of metal ions including Cd 2+ were up to 780-fold compared to a conventional CZE mode using absorbance detection. The detection limits were 17 nM, 15 nM, and 27 nM for Ni 2+, Zn 2+, and Cd 2+ in a 250 mM NaCl matrix, respectively. Our method was successfully applied to the analysis of urine samples without desalting.

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“…Isotachophoresis (ITP) can be used for preconcentration of charged analytes from diluted samples or from samples containing low amounts of analytes (for review see ref. [240]). Micellar electrokinetic chromatography (MEKC) is almost exclusively used for analysis of small molecules such as drug and their metabolites.…”

Section: High-performance Capillary Electrophoresis (Hpce) Of the No mentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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Recent advances of transient isotachophoresis‐capillary electrophoresis in the analysis of small ions from high‐conductivity matrices

Cited by 124 publications

References 110 publications

Is metabolomics reachable? Different purification strategies of human colon cancer cells provide different CE‐MS metabolite profiles

Is metabolomics reachable? Different purification strategies of human colon cancer cells provide different CE‐MS metabolite profiles

Isotachophoretically Assisted On-Line Complexation of Trace Metal Ions in a Highly Saline Matrix for Capillary Electrophoresis

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

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