Recent advances on fluorescent biomarkers of near-infrared quantum dots for<i>in vitro</i>and<i>in vivo</i>imaging

Chinnathambi, Shanmugavel; Shirahata, Naoto

doi:10.1080/14686996.2019.1590731

Cited by 152 publications

(83 citation statements)

References 153 publications

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“…A promising tool are quantum dots (QDs) which can be described as objects of the size below 10 nm with unique properties [1][2][3][4]. Since their development, they have been successfully used in optoelectronics [1], food packaging [5], metals detection [6][7][8], bioimaging [9][10][11][12][13][14], photocatalysis [15,16], sensing [17][18][19][20][21][22][23], cell labelling [24], or fluorescent inks and others [25,26]. The first-generation quantum dots are semiconductors of crystalline structure [3].…”

Section: Introductionmentioning

confidence: 99%

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

et al. 2020

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Carbon quantum dots (CQDs) are nanoobjects of a size below 10 nm. Due to their favorable features, such as tunable luminescence, unique optical properties, water solubility, and lack of cytotoxicity, they are willingly applied in biomedicine. They can be obtained via bottom-up and top-down methods. However, to increase their quantum yield they must undergo post-processing. The aim of the following research was to obtain a new type of CQDs modified with a rhodamine b derivative to enhance their fluorescence performance without biocompability deterioration. For their preparation glucose was used as a precursor and four different carbonizing agents which affected semi-and final products luminescence properties. The ready nanomaterials were investigated over their chemical structure by FTIR and NMR, whereas morphology was investigated by the TEM method. Their optical properties were determined by UV-VIS spectroscopy. Fluorescence behavior, photo-and pH-stability, as well as solvatochromism showed their applicability in various biomedical applications due to the controlled properties. The samples exhibited excellent antioxidant activity and lack of cytotoxicity on L929 mouse fibroblasts. The results showed that proposed strategy enables preparation of the superior nanomaterials with outstanding luminescence properties such as quantum yield up to 17% which can be successfully applied in cell labelling, bioimaging, and theranostics.Molecules 2020, 25, 736 2 of 18 can release cadmium ions leading to cell death. Importantly, it occurred that quantum dots may undergo certain modifications resulting in the change of their surface or chemical structure [27,28]. Finally, their preparation is expensive. Therefore, there was a need for a novel type of quantum dots which would be suitable for biomedical applications. In 2004, a new type of carbon-based materials was accidentally obtained during nanotube purification [29]. Until then, numerous types of carbon quantum dots (CQDs) have been obtained with various possible applications due to their very attractive properties, such as luminesce, good water solubility, high photostability, resistance to photobleaching or photoblinking, possibility, chemical inertness and, finally, lack of toxicity and biocompability [1][2][3]. Furthermore, CQDs' region of photoluminescence is very broad, starting in the ultraviolet and ending in the near-infrared. Although, until now, the luminescence behavior of carbon nanodots is still not fully clear, it is believed that crucial role plays two factors, namely, that the quantum size is below 10 nm and surface defects which may act as excitation energy traps. Interestingly, CQDs can successfully act as both electron donors and acceptors [2][3][4].CQDs can be prepared from various resources, such as peels (mango, orange, banana, pineapple, onion, seaweed etc.), meat, grains, nuts, or vegetable by-products, which makes them a low-cost material [30,31]. Currently, these nanomaterials are widely applied in drug delivery, gene delivery, biosensing, bioimag...

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Section: Introductionmentioning

confidence: 99%

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

et al. 2020

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“…For example, medical multifunctional materials may be designed to enable simultaneous diagnosis An interesting group of photoactive materials is those that exhibit nonlinear optical (NLO) properties, especially those showing large multiphoton absorption cross sections. They are widely considered as optical agents for applications requiring longer excitation wavelengths such as in medical diagnosis, for instance, photodynamic two-photon cancer therapy or two-photon bioimaging, where excitation in the range of biological transmission window (650-900 nm) is preferred [21,22]. In fact, semiconductor nanoparticles are being widely characterized with respect to their NLO characteristics, as they possess significant advantages for the above-mentioned applications such as stability upon high-energy light exposure (no bleaching) and high luminescence quantum yields [23,24].…”

Section: Introductionmentioning

confidence: 99%

Functional CdS-Au Nanocomposite for Efficient Photocatalytic, Photosensitizing, and Two-Photon Applications

et al. 2020

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We demonstrate a low-temperature synthesis of hydrophilic, penicillamine-stabilized hybrid CdS-Au nanoparticles (NPs) utilizing different Au concentrations. The obtained hybrid nanomaterials exhibit photoluminescence quenching and emission lifetime reduction in comparison with their raw semiconductor CdS NPs counterparts. An increase of concentration of Au present at the surface of CdS leads to lower photoluminescence intensity and faster emission decays, suggesting more efficient charge separation when larger Au domains are present. For photocatalysis studies, we performed methylene blue (MB) absorption measurements under irradiation in the presence of CdS-Au NPs. After 1 h of light exposure, we observed the absorbance decrease to about 35% and 10% of the initial value for the CdS-5Au and CdS-7.5Au (the hybrid NPs obtained in a presence of 5.0 and 7.5 mM Au), respectively, which indicates MB reduction caused by electrons effectively separated from holes on metal surface. In further similar photocatalysis experiments, we measured bovine serum albumin (BSA) integrated photoluminescence intensity quenching in the presence of CdS-Au NPs, with a 50% decrease being obtained for CdS-2.5Au NPs and CdS-5Au NPs, with a faster response rate detected for the system prepared with a higher Au concentration. The results suggest hole-driven reactive oxygen species (ROS) production, causing BSA degeneration. Finally, we performed two-photon excited emission (TPEE) measurements for CdS-5Au NPs, obtaining their two-photon absorption (TPA) cross-section values up to 15.8 × 103 GM (Goeppert-Mayer units). We conclude that the obtained water-soluble CdS-Au NPs exhibit potential triple functionalities as photocatalysts for reduction and oxidation reactions as well as materials for two-photon absorption applications, so that they may be considered as future theranostics.

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“…[14,15]. On the other hand, the dominant absorber in the scalp layer is melanin which gradually increase optical attenuation as one gets close to shorter wavelength range [16]. The inversely proportional relationship was identified between Φ " !!!…”

Section: Main Textmentioning

confidence: 98%

Light-emitting diode-based transcranial photoacoustic measurement of sagittal sinus oxyhemoglobin saturation in hypoxic neonatal piglets

Kang

Koehler

Adams

et al. 2020

Preprint

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We present a light-emitting diode (LED)-based transcranial photoacoustic measurement (LED-trPA) of oxyhemoglobin (HbO2) saturation at superior sagittal sinus (SSS) in hypoxic neonatal piglets. The optimal LED imaging wavelengths and frame averaging scheme were determined based on in vivo characterization of transcranial sensitivity. Based on the framework (690/850 nm with >20 frame averaging), graded hypoxia was successfully identified in neonatal piglets in vivo with less than 10.0 % of root mean squared error (RMSE). This preclinical study suggests the feasibility of a rapid, cost-effective, and safe LED-trPA monitoring of perinatal hypoxia-ischemia and prompt interventions for clinical use.

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Recent advances on fluorescent biomarkers of near-infrared quantum dots forin vitroandin vivoimaging

Cited by 152 publications

References 153 publications

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

Smart, Tunable CQDs with Antioxidant Properties for Biomedical Applications—Ecofriendly Synthesis and Characterization

Functional CdS-Au Nanocomposite for Efficient Photocatalytic, Photosensitizing, and Two-Photon Applications

Light-emitting diode-based transcranial photoacoustic measurement of sagittal sinus oxyhemoglobin saturation in hypoxic neonatal piglets

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