Recent advances on<scp>DNA</scp>and omics‐based technology in Food testing and authentication: A review

Kumar, Pramod; Rani, Alka; Singh, Shalini; Kumar, Anuj

doi:10.1111/jfs.12986

Cited by 13 publications

(3 citation statements)

References 104 publications

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“…For the halal authentication of meat products using lipidomic information, quite a lot of data are produced, so that the chemometrics methods used are not only single but also in combination to present information that is easier to understand [ 108 ]. PCA is a chemometric method that is most often combined with HCA or PLS-DA.…”

Section: Chemometrics Applications In Lipidomic Analysis For Halal Au...mentioning

confidence: 99%

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

et al. 2022

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The halal status of meat products is an important factor being considered by many parties, especially Muslims. Analytical methods that have good specificity for the authentication of halal meat products are important as quality assurance to consumers. Metabolomic and lipidomic are two useful strategies in distinguishing halal and non-halal meat. Metabolomic and lipidomic analysis produce a large amount of data, thus chemometrics are needed to interpret and simplify the analytical data to ease understanding. This review explored the published literature indexed in PubMed, Scopus, and Google Scholar on the application of chemometrics as a tool in handling the large amount of data generated from metabolomic and lipidomic studies specifically in the halal authentication of meat products. The type of chemometric methods used is described and the efficiency of time in distinguishing the halal and non-halal meat products using chemometrics methods such as PCA, HCA, PLS-DA, and OPLS-DA is discussed.

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Section: Chemometrics Applications In Lipidomic Analysis For Halal Au...mentioning

confidence: 99%

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

et al. 2022

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“…As, COI gene is conspeci c, so it can e ciently and accurately discriminate between meat species (Ahmed, 2022). Due to its simplicity, high accuracy and e ciency, the use of DNA barcoding for detection of animal species has become much appealing for researchers (Kumar, Rani, Singh, & Kumar, 2022). This molecular approach relies heavily on good quality of genomic DNA extraction.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Extraction of Genomic DNA from Cooked meats and their Amplification

Ahmad

Saleem

Baqi

2023

Preprint

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Mislabeling of meat products is a type of food fraud that can result in economic deception as well as interference with dietary restrictions due to allergens or religious beliefs. The purpose of this study is to describe an efficient DNA extraction method from processed meat. This work devised a procedure for genomic DNA isolation from the cooked meat of mammal species including goat, sheep, cow and buffalo. The predicted PCR products of 658 base pair (bp) were produced by PCR amplification using DNA that was extracted from cooked and processed meat samples. In this research, a high quality DNA was obtained from cooked meat of goat, sheep, cow and buffalo samples and analysis of whole genomic DNA has been carried out in agarose gel. The PCR results showed that the primers employed in this investigation generated bands with sizes between 650 and 700 bp at the annealing temperature of 55°C for all species. The extracted genomic DNA is of high quality to allow precise and accurate PCR amplification of cytochrome c oxidase subunit 1 barcode. In this way, potential adulteration in a variety of meat products will be effectively detected using this technique.

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“…Based on the WGS analysis of pathogens, genotypic characterization using whole/core genome multilocus sequence typing (wg/cgMLST) and whole-genome single nucleotide polymorphism (wgSNP) could be obtained (Kumar et al, 2022). However, which kind of genotypic characterization has higher resolution to distinguish the differences between two consecutive food poisoning outbreaks is still unknown.…”

mentioning

confidence: 99%

Phenotypic and genotypic characterization of salmonella Enteritidis isolated from two consecutive Food‐Poisoning outbreaks in Sichuan, China

Ximei

Chen

Yang

et al. 2022

Journal of Food Safety

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Salmonella enterica serotype Enteritidis (SE) is a primary pathogen that causes foodborne diseases in humans. Although whole‐genome sequencing (WGS) ‐based typing analyses have been increasingly used to investigate food‐poisoning outbreaks, they are rarely applied to the epidemiology of multiple Salmonella outbreaks in Sichuan, China. This study therefore isolated SE from patients and food of two consecutive food‐poisoning outbreaks during 2020 in Sichuan, China. We tracked outbreak origin using epidemiological investigation, serotyping, antimicrobial susceptibility testing (AST), pulsed‐field gel electrophoresis (PFGE), and WGS. We also determined phylogenetic relationships using PFGE, whole and core genome multilocus sequence typing (wg/cgMLST), and whole‐genome single nucleotide polymorphism (wgSNP) analyses. Epidemiological investigations identified a correlation between cake consumption and food poisoning. Thirteen strains isolated from patients and one strain isolated from the cake were confirmed as SE. Among the 14 strains, only six shared the same AST pattern (AMP‐AMS‐Sul‐STR). Isolates from patients and cakes were indistinguishable in PFGE results. All four methods, namely PFGE, wgMLST, cgMLST, and wgSNP were appropriate for bacterial typing in SE‐related outbreak investigation. However, wgSNP can assign 12 SE strains from the first outbreak to one cluster and assign two SE strains from the second outbreak to another cluster, while PFGE, wgMLST, cgMLST did not successfully distinguish the SE strains from different outbreaks. Thus, we conclude that SNP‐based phylogenetic analysis might be a viable method for differentiating SE strains at the outbreak level.

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Recent advances onDNAand omics‐based technology in Food testing and authentication: A review

Cited by 13 publications

References 104 publications

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

Exploring the Extraction of Genomic DNA from Cooked meats and their Amplification

Phenotypic and genotypic characterization of salmonella Enteritidis isolated from two consecutive Food‐Poisoning outbreaks in Sichuan, China

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