Recent advances to accelerate re-endothelialization for vascular stents

Bedair, Tarek M.; Elnaggar, Mahmoud A.; Joung, Yoon Ki; Han, Dong Keun

doi:10.1177/2041731417731546

Cited by 73 publications

(44 citation statements)

References 143 publications

(227 reference statements)

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“…The morphology and microstructure of biomaterial surface play a vital role in guiding and modulating the adhesion and growth of cells . SEM and atomic force microscopy (AFM) were utilized to characterize the surface morphology and microstructure of modified PS surfaces.…”

Section: Resultsmentioning

confidence: 99%

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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Artificial small‐caliber vascular grafts are still limited in clinical application because of thrombosis, restenosis, and occlusion. Herein, a small‐caliber vascular graft (diameter 2 mm) is fabricated from poly(ε‐caprolactone)‐b‐poly(isobutyl‐morpholine‐2,5‐dione) (PCL‐PIBMD) and silk fibroin (SF) by electrospinning technology and then biofunctionalized with low‐fouling poly(ethylene glycol) (PEG) and two cell‐adhesive peptide sequences (CREDVW and CAGW) with the purpose of enhancing antithrombogenic activity and endothelialization. The successful grafting of PEG and peptide sequences is confirmed by X‐ray photoelectron spectroscopy. The suitable surface wettability of the modified vascular graft is testified by water contact angle analysis. The surface hemocompatibility is verified by platelet adhesion assays and protein adsorption assays, and the results demonstrate that both platelet adhesion and protein adsorption on the biofunctionalized surface are significantly reduced. In vitro studies demonstrate that the biofunctionalized surface with suitable hydrophilicity and cell‐adhesive peptides can selectively promote the adhesion, spreading, and proliferation of human umbilical vein endothelial cells. More importantly, compared with control groups, this biofunctionalized small‐caliber vascular graft shows high long‐term patency and endothelialization after 10 weeks of implantation. The biofunctionalization with PEG and two cell‐adhesive peptide sequences is an effective method to improve the endothelialization and long‐term performance of synthetic vascular grafts.

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Section: Resultsmentioning

confidence: 99%

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Bai

Zhao

et al. 2018

Macromolecular Bioscience

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“…These results clearly demonstrate the distinctive role of nanotopography in cellular adhesion and proliferation, with a preferential cell response. 12 , 13 Such a preferential response is reported on titania surfaces having nanoscale topography (e.g., nanotubes). 19 …”

Section: Resultsmentioning

confidence: 81%

“…These stents (TITAN) showed a significant reduction in neointimal hyperplasia in comparison to bare SS in porcine model 8 and in clinical trials. 9 − 11 Additionally, topographical modifications at the nanoscale, 12 − 14 including studies from our own group, have demonstrated the success of surface-modified SS 15 and titanium (Ti) 16 , 17 substrates in promoting endothelial cell proliferation. Research has shown that titanium surfaces having submicron patterns with lateral dimensions >100 nm could efficiently promote endothelial cell adhesion, 18 whereas titanium dioxide (TiO 2 ) nanostructures displayed a concomitant reduction in smooth muscle cell (SMC) proliferation with good endothelialization in vitro.…”

Section: Introductionmentioning

confidence: 99%

Successful Reduction of Neointimal Hyperplasia on Stainless Steel Coronary Stents by Titania Nanotexturing

et al. 2020

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Bare metal stents (BMSs) of stainless steel (SS) were surface engineered to develop nanoscale titania topography using a combination of physical vapor deposition and thermochemical processing. The nanoleafy architecture formed on the stent surface remained stable and adherent upon repeated crimping and expansion, as well as under flow. This titania nanoengineered stent showed a preferential proliferation of endothelial cells over smooth muscle cells in vitro, which is an essential requirement for improving the in vivo endothelialization, with concurrent reduction of intimal hyperplasia. The efficacy of this surface-modified stent was assessed after implantation in rabbit iliac arteries for 8 weeks. Significant reduction in neointimal thickening and thereby in-stent restenosis with complete endothelial coverage was observed for the nanotextured stents, compared to BMSs, even without the use of any antiproliferative agents or polymers as in drug-eluting stents. Nanotexturing of stents did not induce any inflammatory response, akin to BMSs. This study thus indicates the effectiveness of a facile titania nanotopography on SS stents for coronary applications and the possibility of bringing this low-priced material back to clinics.

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“…Several groups have reported different functionalization strategies via the use of coatings and chemical and protein modifications to promote the endothelialization of the scaffold [59,71,72,92]. For instance, different molecules such as fucoidan, heparin, chondroitin sulfate, hyaluronic acid (HA), as well as antioxidant compounds and ECM proteins including fibronectin, laminin, and collagen have been used to improve cell-material interactions to promote endothelialization [93]. These studies have demonstrated that surface modification often leads to increased endothelial cell attachment and proliferation, as well as overexpression of endothelial markers such as smooth muscle myosin heavy chain (SM-MHC) [94].…”

Section: Biomimetic Design Of Biomaterials For Cardiovascular Tementioning

confidence: 99%

Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation

et al. 2019

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Bioengineered tissues have become increasingly more sophisticated owing to recent advancements in the fields of biomaterials, microfabrication, microfluidics, genetic engineering, and stem cell and developmental biology. In the coming years, the ability to engineer artificial constructs that accurately mimic the compositional, architectural, and functional properties of human tissues, will profoundly impact the therapeutic and diagnostic aspects of the healthcare industry. In this regard, bioengineered cardiac tissues are of particular importance due to the extremely limited ability of the myocardium to self-regenerate, as well as the remarkably high mortality associated with cardiovascular diseases worldwide. As novel microphysiological systems make the transition from bench to bedside, their implementation in high throughput drug screening, personalized diagnostics, disease modeling, and targeted therapy validation will bring forth a paradigm shift in the clinical management of cardiovascular diseases. Here, we will review the current state of the art in experimental in vitro platforms for next generation diagnostics and therapy validation. We will describe recent advancements in the development of smart biomaterials, biofabrication techniques, and stem cell engineering, aimed at recapitulating cardiovascular function at the tissue- and organ levels. In addition, integrative and multidisciplinary approaches to engineer biomimetic cardiovascular constructs with unprecedented human and clinical relevance will be discussed. We will comment on the implementation of these platforms in high throughput drug screening, in vitro disease modeling and therapy validation. Lastly, future perspectives will be provided on how these biomimetic platforms will aid in the transition towards patient centered diagnostics, and the development of personalized targeted therapeutics.

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Recent advances to accelerate re-endothelialization for vascular stents

Cited by 73 publications

References 143 publications

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Biofunctionalized Electrospun PCL‐PIBMD/SF Vascular Grafts with PEG and Cell‐Adhesive Peptides for Endothelialization

Successful Reduction of Neointimal Hyperplasia on Stainless Steel Coronary Stents by Titania Nanotexturing

Biomimetic cardiovascular platforms for in vitro disease modeling and therapeutic validation

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