Recent advances towards single biomolecule level understanding of protein adsorption phenomena unique to nanoscale polymer surfaces with chemical variations

Cho, David H.; Xie, Tian; Truong, Johnson; Stoner, Andrew C.; Hahm, Jong‐in

doi:10.1007/s12274-020-2735-7

Cited by 13 publications

(23 citation statements)

References 196 publications

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“…Interaction behaviors of a model protein, immunoglobulin G (IgG), were subsequently examined under the confinements imposed by the nanoscale polymer interfaces. This seminal and other ensuing research efforts have led to key discoveries important for understanding protein interactions at nanoscale polymer interfaces that are distinctively different from their interactions with macro- and bulk-scale, chemically unvarying polymers. ,− …”

Section: First Step Toward Understanding Protein Interactions At Nano...mentioning

confidence: 99%

Protein–Polymer Interaction Characteristics Unique to Nanoscale Interfaces: A Perspective on Recent Insights

Cho

Hahm

2021

J. Phys. Chem. B

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Protein interactions at polymer interfaces represent a complex but ubiquitous phenomenon that demands an entirely different focus of investigation than what has been attempted before. With the advancement of nanoscience and nanotechnology, the nature of polymer materials interfacing proteins has evolved to exhibit greater chemical intricacy and smaller physical dimensions. Existing knowledge built from studying the interaction of macroscopic, chemically alike surfaces with an ensemble of protein molecules cannot be simply carried over to nanoscale protein− polymer interactions. In this Perspective, novel protein interaction phenomena driven by the presence of nanoscale polymer interfaces are discussed. Being able to discern discrete protein interaction events via simple visualization was crucial to attaining the much needed, direct experimental evidence of protein−polymer interactions at the single biomolecule level. Spatial and temporal tracking of particular proteins at specific polymer interfaces was made possible by resolving individual proteins simultaneously with those polymer nanodomains responsible for the protein interactions. Therefore, such single biomolecule level approaches taken to examine protein−polymer interaction mark a big departure from the mainstream approaches of collecting indirectly observed, ensembleaveraged protein signals on chemically simple substrates. Spearheading research efforts so far has led to inspiring initial discoveries of protein interaction mechanisms and kinetics that are entirely unique to nanoscale polymer systems. They include protein selfassembly/packing characteristics, protein−polymer interaction mechanisms/kinetics, and various protein functionalities on polymer nanoconstructs. The promising beginning and future of nanoscale protein−polymer research endeavors are presented in this article.

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Section: First Step Toward Understanding Protein Interactions At Nano...mentioning

confidence: 99%

Protein–Polymer Interaction Characteristics Unique to Nanoscale Interfaces: A Perspective on Recent Insights

Cho

Hahm

2021

J. Phys. Chem. B

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“…This theoretical study is based on the simulation protocol for protein adsorption on biomaterial surfaces proposed in previous work [19][20][21][22]65] that involves three sequential steps:…”

Section: Resultsmentioning

confidence: 99%

“…In many different fields of nanomedicine, nanobiotechnology, biomaterial science [10][11][12], protein-biomaterials interactions are a central aspect in a broad range of applications such as drug delivery [13], tissue engineering [14][15][16][17], proteomics [18,19]. Biomaterials are composed by several different materials such as ceramics, polymers, metals and metal oxides, and of their combinations in interesting new biocompatible composite materials [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Raffaini

2021

Coatings

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TiO2 is widely used in biomaterial implants. The topography, chemical and structural properties of titania surfaces are an important aspect to study. The size of TiO2 nanoparticles synthetized by sol–gel method can influence the responses in the biological environment, and by using appropriate heat treatments different contents of different polymorphs can be formed. Protein adsorption is a crucial step for the biological responses, involving, in particular, albumin, the most abundant blood protein. In this theoretical work, using molecular mechanics and molecular dynamics methods, the adsorption process of an albumin subdomain is reported both onto specific different crystallographic faces of TiO2 anatase and also on its ideal three-dimensional nanosized crystal, using the simulation protocol proposed in my previous theoretical studies about the adsorption process on hydrophobic ordered graphene-like or hydrophilic amorphous polymeric surfaces. The different surface chemistry of anatase crystalline faces and the nanocrystal topography influence the adsorption process, in particular the interaction strength and protein fragment conformation, then its biological activity. This theoretical study can be a useful tool to better understand how the surface chemistry, crystal structure, size and topography play a key role in protein adsorption process onto anatase surface so widely used as biomaterial.

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“…The cited results and references show that there is a large number of engineered nanosystems with antifouling properties. However, the experimental information is disperse and heterogeneous [11,37,38]. Recent efforts combine NP libraries with the development of nanodescriptors to find correlations that would accelerate the discovery of NPs with tunable properties [39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Proteins Adsorbing onto Surface-Modified Nanoparticles: Effect of Surface Curvature, pH, and the Interplay of Polymers and Proteins Acid–Base Equilibrium

2022

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Protein adsorption onto nanomaterials is a process of vital significance and it is commonly controlled by functionalizing their surface with polymers. The efficiency of this strategy depends on the design parameters of the nanoconstruct. Although significant amount of work has been carried out on planar surfaces modified with different types of polymers, studies investigating the role of surface curvature are not as abundant. Here, we present a comprehensive and systematic study of the protein adsorption process, analyzing the effect of curvature and morphology, the grafting of polymer mixtures, the type of monomer (neutral, acidic, basic), the proteins in solution, and the conditions of the solution. The theoretical approach we employed is based on a molecular theory that allows to explicitly consider the acid–base reactions of the amino acids in the proteins and the monomers on the surface. The calculations showed that surface curvature modulates the molecular organization in space, but key variables are the bulk pH and salt concentration (in the millimolar range). When grafting the NP with acidic or basic polymers, the surface coating could disfavor or promote adsorption, depending on the solution’s conditions. When NPs are in contact with protein mixtures in solution, a nontrivial competitive adsorption process is observed. The calculations reflect the balance between molecular organization and chemical state of polymers and proteins, and how it is modulated by the curvature of the underlying surface.

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Recent advances towards single biomolecule level understanding of protein adsorption phenomena unique to nanoscale polymer surfaces with chemical variations

Cited by 13 publications

References 196 publications

Protein–Polymer Interaction Characteristics Unique to Nanoscale Interfaces: A Perspective on Recent Insights

Protein–Polymer Interaction Characteristics Unique to Nanoscale Interfaces: A Perspective on Recent Insights

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Proteins Adsorbing onto Surface-Modified Nanoparticles: Effect of Surface Curvature, pH, and the Interplay of Polymers and Proteins Acid–Base Equilibrium

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