Recent Applications and Future Perspectives of Chemiluminescent and Bioluminescent Imaging Technologies

Abstract

Imaging technologies based on chemiluminescence (CL) and bioluminescence (BL) have seen a tremendous growth in the past decade due to their extensive contributions to biochemical analysis and biomedical science. CL and BL imaging technologies have many advantages over commonly used imaging techniques (such as fluorescence and electrochemical systems). CL and BL proceed without an external light source, thus avoiding photobleaching, high interference of background signal, and autofluorescence. Furthermore, CL and BL analytical techniques are characterized by their low detection limit, high selectivity, short assay time, and simple instrumentation. Recently, CL-based imaging technology has been applied successfully for the determination and in vivo imaging of several significant analytes. Meanwhile, an innovative BL imaging technology has been established for the noninvasive real-time tracking of different biomolecules relying on the interaction between luciferase and its substrate. In the current review, we discuss the recent applications of CL and BL imaging approaches over the past five years. Finally, we also discuss the current state of progress and the prospects for CL and BL imaging systems.

Protein-Templated Metal Nanoclusters: Molecular-like Hybrids for Biosensing, Diagnostics and Pharmaceutics

“…The CL phenomenon has been widely employed for various biomedical applications such as bioimaging [ 80 , 81 ], biosensing [ 82 ] and therapeutics [ 83 ]. The CL of protein-templated metal NCs is mainly from their role as catalysts, while their direct use as a luminophore or quencher is comparatively less.…”

Optical Phenomena in Molecule-Based Magnetic Materials

“…Among other optical materials, extensive research works are focused on the design and investigation of the solids exhibiting luminescence that is the light emission occurring due to the absorption of photons (photo­luminescence), , electric current (electro­luminescence), chemical reaction (chemi­luminescence), ionizing radiation (radio­luminescence), or mechanical action (mechano­luminescence) . They found vital applications ranging from light-emitting diodes (LEDs), optical communication, and photovoltaics, to sensors of physical and chemical stimuli, anti­counterfeiting as well as bioimaging tools. ,− Luminescence can be realized by playing with the emissive electronic transitions generated in inorganic solids, such as oxides or fluorides, , organic molecules and frameworks, , as well as complexes of lanthanides or transition metals, including both heavy elements as well as earth abundant 3d metals, ,− coordination polymers, including metal–organic frameworks (MOFs), , and molecular organic-inorganic hybrids . The vast part of their applications is related to the construction of sensors which is due to the sensitivity of these materials to external stimuli, especially for molecule-based systems .…”

A mitochondria-targeted chemiluminescent probe for detection of hydrogen sulfide in cancer cells, human serum and in vivo

“…[78][79][80] Additionally, they bear a modular scaffold, which can be simply modified toward the development of activity-based probes. Accordingly, new generation 1,2-dioxetane derivatives have been developed to monitor numerous analytes ranging from enzymes to ROS and biothiols including H 2 S. [81][82][83][84][85][86] Very recently, we have introduced the first ever example of a mitochondria-targeted chemiluminescence agent by modifying the phenoxy 1,2-dioxetane core, which was responsive to the leucine aminopeptidase (LAP) enzyme, with a well-established mitochondrion targeting triphenylphosphonium (TPP) group. 87 Cationic nature of TPP is critical to ensure the attraction between the probe and the negatively charged membrane of mitochondria as previously shown in the literature.…”