Recent Applications of Mass Spectrometry at Clarkson University

Jayathirtha, Madhuri; Whitham, Danielle; Stradtman, Sydney; Darie, Costel C.

doi:10.1007/978-3-030-15950-4_46

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…81 In the case of mass spectrometry, most technicians and lab staff do not have a background in analytical chemistry and are less familiar with mass spectrometry. 83 Often, they have to be introduced to the complex analysis of quantitative bottom-up proteomics. MS-based quantitation of protein biomarkers is specialized work, which requires a thorough educational process that should be provided to employees at different levels from hands on technical staff to those dealing with management and directing biomarker quantitation for clinical utility.…”

Section: Development and Analytical Performance Evaluationmentioning

confidence: 99%

“…To ensure that medical tests provide accurate clinical information, ISO guidelines require in general proper training of the work force on the procedures needed for the application . In the case of mass spectrometry, most technicians and lab staff do not have a background in analytical chemistry and are less familiar with mass spectrometry . Often, they have to be introduced to the complex analysis of quantitative bottom-up proteomics.…”

Section: Achieving Long-term Robustness: Implementationmentioning

confidence: 99%

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The Time Has Come for Quantitative Protein Mass Spectrometry Tests That Target Unmet Clinical Needs

Smit

Ruhaak

Romijn

et al. 2021

J. Am. Soc. Mass Spectrom.

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Protein mass spectrometry (MS) is an enabling technology that is ideally suited for precision diagnostics. In contrast to immunoassays with indirect readouts, MS quantifications are multiplexed and include identification of proteoforms in a direct manner. Although widely used for routine measurements of drugs and metabolites, the number of clinical MS-based protein applications is limited. In this paper, we share our experience and aim to take away the concerns that have kept laboratory medicine from implementing quantitative protein MS. To ensure added value of new medical tests and guarantee accurate test results, five key elements of test evaluation have been established by a working group within the European Federation for Clinical Chemistry and Laboratory Medicine. Moreover, it is emphasized to identify clinical gaps in the contemporary clinical pathways before test development is started. We demonstrate that quantitative protein MS tests that provide an additional layer of clinical information have robust performance and meet long-term desirable analytical performance specifications as exemplified by our own experience. Yet, the adoption of quantitative protein MS tests into medical laboratories is seriously hampered due to its complexity, lack of robotization and high initial investment costs. Successful and widespread implementation in medical laboratories requires uptake and automation of this next generation protein technology by the In-Vitro Diagnostics industry. Also, training curricula of lab workers and lab specialists should include education on enabling technologies for transitioning to precision medicine by quantitative protein MS tests.

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Section: Development and Analytical Performance Evaluationmentioning

confidence: 99%

Section: Achieving Long-term Robustness: Implementationmentioning

confidence: 99%

The Time Has Come for Quantitative Protein Mass Spectrometry Tests That Target Unmet Clinical Needs

Smit

Ruhaak

Romijn

et al. 2021

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

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Mass Spectrometric (MS) Analysis of Proteins and Peptides

Jayathirtha

Dupree

Manzoor

et al. 2021

CPPS

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: The human genome is sequenced and is comprised of~30,000 genes, making humans just a little bit more complicated than worms or flies. However, complexity of humans is given by proteins that these genes code for, because one gene can produce many proteins mostly through alternative splicing and tissue-dependent expression of particular proteins. In addition, post-translational modifications (PTMs) in proteins greatly increase the number of gene products or protein isoforms. Furthermore, stable and transient interactions between proteins, protein isoforms/proteoforms and PTM-ed proteins (proteinprotein interactions, PPI) adds yet another level of complexity in humans and other organisms. In the past, all of these proteins were analyzed one at the time. Currently, they are analyzed by a less tedious method: mass spectrometry (MS) for two reasons: 1) because of the complexity of proteins, protein PTMs and PPIs and 2) because MS is the only method that can keep up with such a complex array of features. Here, we discuss the applications of mass spectrometry in protein analysis.

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Recent Applications of Mass Spectrometry at Clarkson University

Cited by 2 publications

References 17 publications

The Time Has Come for Quantitative Protein Mass Spectrometry Tests That Target Unmet Clinical Needs

The Time Has Come for Quantitative Protein Mass Spectrometry Tests That Target Unmet Clinical Needs

Mass Spectrometric (MS) Analysis of Proteins and Peptides

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