Recent Applications of Phosphoproteomics

Abstract

Protein phosphorylation plays a key role in the regulation of most cellular processes and the disorder of this process often leads to illness or even cancers. Panoramic phosphorylation analysis of organisms known as "phosphoproteomics" has become more important and popular in scientific research fields these days due to its ability of gaining significant information about phosphorylation events and its assistance in understanding how those regulatory functions work in cell. Analytical strategies have been developed for comprehensive analysis of phosphoproteome over the past several years on the aspects of both profiling and quantification. In this review, we will discuss some of the recent applications of phosphoproteomics by using several commonly used technologies, and the applications on study of cell signal transduction pathways and disease-related biomarker and drug targets discovery will also be covered.

Fabrication of functionalized SiO2/TiO2 nanocomposites via amidation for the fast and selective enrichment of phosphopeptides

“…Reversible protein phosphorylation is regarded as the most common post-translational modification in the regulation of cellular activities, [1][2][3] and is involved in a variety of regulatory functions such as cell growth, division and proliferation, and signal transduction. [4][5][6][7][8][9][10] At present, mass spectrometry, especially matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), is usually used to characterize phosphoproteins.…”

Amine-Functionalized Sol–Gel-Based Lab-in-a-Pipet-Tip Approach for the Fast Enrichment and Specific Purification of Phosphopeptides in MALDI-MS Applications

“…Post-translational modifications of protein structures control several biological activities. Therefore, examining the roles of these modifications in the mechanisms of cellular regulation is important. − Phosphorylation is the reversible covalent binding of a phosphate group to one or more amino acids; this is the most common post-translational modification in the regulation of cellular activities. It is involved in many regulatory functions, such as cell cycle control, signal transduction, differentiation, proliferation, transformation, and metabolism .…”